Enrichment of fetal trophoblast cells from the maternal peripheral blood followed by detection of fetal deoxyribonucleic acid with a nested X/Y polymerase chain reaction

Wijk, Inge J. van; Vugt, J.M.G. van; Mulders, Monique A.M.; Könst, Andrea A.M.; Weima, Sjerp M.; Oudejans, Cees B.M.

doi:10.1016/s0002-9378(96)70315-0

Cited by 52 publications

(27 citation statements)

References 20 publications

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“…23,26,28 A few published studies of fetal NRBC sorting have quoted values 10 times this number. Studies which count fetal cells based only on staining, or morphological criteria probably overestimate the total number of cells because no fetal cell recognition system is completely specific.…”

Section: Cell Numbersmentioning

confidence: 98%

Harvesting Fetal Cells From the Maternal Circulation

McEwan

2005

Fet. Matern. Med. Rev.

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Schmorl is generally credited with being the first to report finding fetal cells in maternal blood. In 1893 he identified trophoblasts in the pulmonary circulation of women who had died of eclampsia. Years later, in 1969, Walnowska identified Y chromosomes in lymphocytes isolated from the blood of pregnant women carrying male fetuses and this was repeated by Herzenberg in 1979 in white blood cells recognised as fetal by their surface HLA-A2 expression. Other sporadic reports followed but not until the 1990's did investigation into harvesting fetal cells from maternal blood begin in earnest. The aim of this article is to review the progress made in isolating and analysing these cells for the purposes of prenatal diagnosis.

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Section: Cell Numbersmentioning

confidence: 98%

Harvesting Fetal Cells From the Maternal Circulation

McEwan

2005

Fet. Matern. Med. Rev.

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“…The consequent global increase of myocardial blood flow suggests functional extracardiac control by humoral and/or neurogenic factors [50,72]. The selective increase in right ventricular afterload may shift cardiac output towards the left ventricle, thus leading to a passive increase of myocardial perfusion [15,17,74,87]. However, the most striking changes in myocardial blood flow can be observed in fetuses subjected to acute hypoxemia after a period of chronic hypoxemia.…”

Section: Coronary Blood Flow Under Various Conditions In the Animal Mmentioning

confidence: 99%

“…With additional centralization, or the development of late decelerations on the fetal heart rate tracing, significantly lower oxygen content and pH values are associated with a concomitant rise in carbon dioxide and lactate in cord artery blood [14,94,95]. With the loss or reversal of end-diastolic flow in the umbilical artery a significant worsening of hypoxemia and acidemia can be detected [ Animal studies using invasive techniques [15,17,74,87] and noninvasive studies using Doppler sonography in the human fetus indicate that intrauterine hypoxemia due to uteroplacental insufficiency is associated with a redistribution in cardiac output in favor of the left ventricle providing preferential perfusion of the brain as well as the myocardium [2,68,76,77]. This is achieved by a relative decrease of left ventricular afterload in the presence of high right ventricular afterload caused by high resistance in the fetoplacental circulation.…”

Section: The Fetal Circulation In Intrauterine Growth Restrictionmentioning

confidence: 99%

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Review articles

Gardosi

1998

Jpme

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Individually adjusted or 'customised' growth charts aim to optimise the assessment of fetal growth by taking individual variation into account, and by projecting an optimal curve which delineates the potential weight gain in each pregnancy. This results in an increased detection rate of true growth restriction and a reduction in false positive diagnoses for IUGR. An adjustable standard can apply across geographical boundaries, as individual variation exceeds that between different maternity populations.

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“…The usual approach is ascertainment of specific target cell populations, most often the fetal nucleated red blood cells (NRBCs) (5,13,14,16,26) or trophobast cells (25,40). Since NRBCs are present in the maternal blood at frequencies as low as 1 per 10 4 -10 8 nucleated cells, depending on methodology and gestational age (3), many enrichment and identification strategies have been developed.…”

mentioning

confidence: 99%