Enrichment of denitratating bacteria from a methylotrophic denitrifying culture

Abstract

Denitratation (nitrite produced from nitrate), has the potential applications in wastewater treatment by combining with ANAMMOX process. The occurrence of denitratation has been shown to be effected qualitatively by various parameters in the environment. A more quantitative understanding can be obtained using enrichment cultures in lab-scale experiments, yet information on the enrichment of functional microorganisms responsible for denitratation is lacking. In this study, a stable denitratation-dominated culture was obtained from methylotrophic denitrifying culture. The results showed that, besides the substitution of acetate for methanol, the lasting starvation following saturation of electron donor was another pivotal selection pressure that favored the growth of denitratating bacteria, which was supported by the distinctive physiological strategy involving the higher growth rate combining with larger poly-hydroxybutyrate (PHB) accumulation at sufficient electron donor situation and then manage the stress of electron donor starvation by consumpiton of the PHB. High-throughput 16S rRNA gene sequencing analysis indicated that non-methylotrophic Halomonas campisalis (48.1 %) and Halomonas campaniensis (30.4 %) dominated in the denitratating community. Moreover the denitratation was driven by the nitrate inhibiting the nirS transcription in the Halomonas species.

Glycerol-driven denitratation: process kinetics, microbial ecology, and operational controls

“…), such as those resulting from fertilizer 2 and explosives manufacturing, 3,4 provide similar energy and chemical reduction opportunities through distinct short-cut BNR processes. A particularly effective pathway for treating waste streams containing both NH 4 + and NO 3 − is through heterotrophic [5][6][7][8][9][10] or autotrophic 11 denitratation (selective reduction of NO , such as those previously described, would theoretically reduce aeration energy requirements by 100% and COD requirements by 60% compared to treatment of the same waste stream using conventional BNR. 12 These benefits translate to municipal wastewater treatment as well, with a 50% decrease in aeration energy and 80% in COD requirements for a partial nitrification-denitratation-anammox system.…”

Low temperature, autotrophic microbial denitrification using thiosulfate or thiocyanate as electron donor

“…organic carbon, ferrous iron (Fe 2+ ), or sulfide (S 2− )] to NO 3 − reduction that if completely reduced, eventually forms nitrogen gas (Lu et al 2014). Recent work has confirmed that organic carbon compounds such as methanol and acetate favor specific denitrifying bacteria and successfully removes NO 3 − (Li et al 2016a). However, the use of methanol as an electron donor produces CaCO 3 , which increases the pH, and affects the denitrifying microbial community in the system.…”

How the Soil Microbial Communities and Activities Respond to Long-Term Heavy Metal Contamination in Electroplating Contaminated Site

“…DNA concentration and purity were determined with microspectrophotometry (NanoDrop ® ). Extracted DNA was sent to Mingke Biotechnology (Hangzhou) Co., Ltd, amplified with 341F (5′-CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGACTACNNGGGTATCTAAT-3′) [22,23]. The purified amplicons were then sequenced on an Illumina Miseq sequencing platform.…”