Enrichment of bacteria and alginate lyase genes potentially involved in brown alga degradation in the gut of marine gastropods

Ito, Motohiro; Watanabe, Kotaro; Maruyama, Toru; Mori, Tetsushi; Niwa, Koichi; Chow, Seinen; Takeyama, Haruko

doi:10.1038/s41598-018-38356-y

Cited by 17 publications

(10 citation statements)

References 56 publications

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“…discus [ 110 ], the Pacific periwinkle L . keenae (micrograzer, [ 51 ]), and others [ 111 ]. Psychrilyobacter can metabolize simple mono- and oligosaccharides (glucose, fructose, N-acetyl-glucosamine, etc.…”

Section: Discussionmentioning

confidence: 99%

Divergence together with microbes: A comparative study of the associated microbiomes in the closely related Littorina species

et al. 2021

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Any multicellular organism during its life is involved in relatively stable interactions with microorganisms. The organism and its microbiome make up a holobiont, possessing a unique set of characteristics and evolving as a whole system. This study aimed to evaluate the degree of the conservativeness of microbiomes associated with intertidal gastropods. We studied the composition and the geographic and phylogenetic variability of the gut and body surface microbiomes of five closely related sympatric Littorina (Neritrema) spp. and a more distant species, L. littorea, from the sister subgenus Littorina (Littorina). Although snail-associated microbiomes included many lineages (207–603), they were dominated by a small number of OTUs of the genera Psychromonas, Vibrio, and Psychrilyobacter. The geographic variability was greater than the interspecific differences at the same collection site. While the microbiomes of the six Littorina spp. did not differ at the high taxonomic level, the OTU composition differed between groups of cryptic species and subgenera. A few species-specific OTUs were detected within the collection sites; notably, such OTUs never dominated microbiomes. We conclude that the composition of the high-rank taxa of the associated microbiome (“scaffolding enterotype”) is more evolutionarily conserved than the composition of the low-rank individual OTUs, which may be site- and / or species-specific.

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Section: Discussionmentioning

confidence: 99%

Divergence together with microbes: A comparative study of the associated microbiomes in the closely related Littorina species

et al. 2021

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“…japonica (70.58%), NORTH AND MINTON 108 bacteria in Firmicutes showed the highest relative abundance, with Proteobacteria being the next most abundant (14.75% and 17.02%, respectively). Proteobacteria have been observed to be the most abundant microbial phylum in the gut of terrestrial, marine, and freshwater snails (Pawar et al 2012;Lyra et al 2018;Ito et al 2019). Of the snail species whose gut flora have been studied, the majority are either herbivores or periphyton grazers/scrapers (e.g., Achatina fulica, Biomphalaria glabrata, Radix auricularia, and Batillus cornutus).…”

Section: Discussionmentioning

confidence: 99%

Diversity and Predicted Function of Gut Microbes from Two Species of Viviparid Snails

North

Minton

2021

Freshwater Mollusk Biology and Conservation

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Animal gut bacteria are involved in numerous critical functions. In snails, gut bacteria play crucial roles in organic material digestion and nutrient production and have been implicated in aspects of reproduction. Snail gut microbes are known to differ between species and even between anatomical compartments of the digestive tract; dietary changes are also known to alter snail gut flora. In an effort to better understand their diversity and function, we studied the gut microbial communities from two viviparid snails, Campeloma decisum and Cipangopaludina japonica. We were interested in whether significant differences in bacterial community composition existed between the two species, and whether differences in microbial diversity corresponded to differences in community function. Using next-generation sequencing of the bacterial 16S V4 region, we found no significant differences in alpha and beta diversity between Ca. decisum and Ci. japonica. Firmicutes and Proteobacteria were the most abundant bacterial phyla in both species, while Bacteroidetes had a higher mean abundance in Ci. japonica. Nine taxonomic groups were present in significantly different mean abundances between the snail species. Pseudomonads and Enterobacteriaceae were notably more abundant in Ca. decisum, while Proteobacteria and Chitinophagaceae were more abundant in Ci. japonica. Peptidoglycan synthesis, pyruvate fermentation, and aerobic respiration by cytochrome c were the three most abundant microbial pathways represented in the viviparid gut. Fourteen functional pathways differed significantly between Ca. decisum and Ci. japonica, potentially correlated with differences in bacterial community composition and snail life history. Our data fill in data gaps regarding gut microbes in Viviparidae and highlight future research paths examining the prevalence of Firmicutes and unidentified diversity in both snail species.

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“…In the present study, they were estimated to be 9%. Some studies relate these bacteria to the biodegradation of complex polysaccharides in the marine environment (Ito et al, 2019; Li et al, 2016). This was not investigated here but it is a probable reason for their significant share in the mucus microbiome.…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity of garden snail mucus

et al. 2022

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The search for new natural compounds for application in medicine and cosmetics is a trend in biotechnology. One of the sources of such active compounds is the snail mucus. Snail physiology and the biological activity of their fluids (especially the mucus) are still poorly studied. Only a few previous studies explored the relationship between snails and their microbiome. The present study was focused on the biodiversity of the snail mucus used in the creation of cosmetic products, therapeutics, and nutraceuticals. The commonly used cultivation techniques were applied for the determination of the number of major bacterial groups. Fluorescence in situ hybridization for key taxa was performed. The obtained images were subjected to digital image analysis. Sequencing of the 16S rRNA gene was also done. The results showed that the mucus harbors a rich bacterial community (10.78 × 1010 CFU/ml). Among the dominant bacteria, some are known for their ability to metabolize complex polysaccharides or are usually found in soil and plants (Rhizobiaceae, Shewanella, Pedobacter, Acinetobacter, Alcaligenes). The obtained data demonstrated that the snail mucus creates a unique environment for the development of the microbial community that differs from other parts of the animal and which resulted from the combined contribution of the microbiomes derived from the soil, plants, and the snails.

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Enrichment of bacteria and alginate lyase genes potentially involved in brown alga degradation in the gut of marine gastropods

Cited by 17 publications

References 56 publications

Divergence together with microbes: A comparative study of the associated microbiomes in the closely related Littorina species

Divergence together with microbes: A comparative study of the associated microbiomes in the closely related Littorina species

Diversity and Predicted Function of Gut Microbes from Two Species of Viviparid Snails

Microbial diversity of garden snail mucus

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