Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial

Kølle, Stig-Frederik Trojahn; Fischer‐Nielsen, Anne; Mathiasen, Anders Bruun; Elberg, Jens Jørgen; Oliveri, Roberto S; Glovinski, Peter V.; Kastrup, Jens; Kirchhoff, Maria; Rasmussen, Birthe Krogh; Talman, Maj Lis Møller; Thomsen, Carsten; Dickmeiss, Ebbe; Drzewiecki, Krzysztof T.

doi:10.1016/s0140-6736(13)61410-5

Cited by 484 publications

(369 citation statements)

References 28 publications

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“…In the present study, we induced T2DM in a rat model by a high-fat diet combined with STZ administration and performed ASC infusion at the 28 th day after STZ injection. We found that ASC infusion could effectively ameliorate hyperglycemia in T2DM rats, which was consistent with other reports [1,2,23]. Furthermore, our results of hyperinsulinemic-euglycemic clamp studies demon-INSR in skeletal muscle and adipose and liver tissues after therapy to investigate the possible mechanisms involved in the effect of ASC infusion on insulin sensitivity.…”

Section: A B Csupporting

confidence: 91%

“…Adipose tissues were then digested with 0.1% type II collagenase for 1 h at 37ºC on a shaker (200 rpm). After 2 washes by centrifugation at 1500 rpm for 5 min in PBS, cells were plated in 75-cm 2 tissue-culture flasks in low glucose DMEM (LG-DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum. Cells were kept in a humidified 5% CO 2 incubator at 37ºC, medium was refreshed every 3-4 days.…”

Section: Isolation and Culture Of Ascmentioning

confidence: 99%

“…ASC can be isolated from small amounts of adipose tissue, efficiently expanded to achieve a very large number after 3 to 4 passages, and have the advantage of low immunogenicity and high multipotency. Their immunmodulatory function, homing and migratory patterns, as well as previous clinical trials, have suggested that these cells are efficient and safe for treating several classes of autoimmune diseases [1][2][3][4].…”

mentioning

confidence: 95%

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Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Chen

et al. 2015

Endocr J

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Type 2 diabeTes melliTus (T2DM) accounts for more than 90% of all diabetic patients and is characterized by insulin resistance, hyperglycemia, systemic low-grade inflammation, and relative lack of insulin. Most T2DM patients experience β-cell exhaustion several years after diagnosis, and thus have to accept insulin therapy. However, insulin therapy is inconvenient for patients and does not completely prevent the development of diabetic complications. Stem cells are an attractive option to ameliorate diabetes for its abundant source and potential to acquire glucose-dependent insulin secretory function. Adult stem cells are an especially good candidate for its safety in terms of tumorigenicity and ethical concerns.Adipose-derived stem cells (ASC) have been stud- The effects and possible mechanisms of adipose-derived stem cells (ASC) infusion on type 2 diabetic rats were investigated in this study. Twenty normal male Sprague-Dawley rats were included in normal control group, and 40 male diabetic rats were randomly divided into diabetic control group and ASC group (which received ASC infusion). After therapy, levels of fasting plasma glucose (FPG), HbA1c, serum insulin and C-peptide, recovery of islet cells, inflammatory cytokines, and insulin sensitivity were analyzed. After ASC infusion, compared with diabetic control group, hyperglycemia in ASC group was ameliorated in 2 weeks and maintained for about 6 weeks, and plasma concentrations of insulin and C-peptide were significantly improved (P<0.01). Number of islet β cells and concentration of vWF in islets in ASC group increased, while activity of caspase-3 in islets was reduced. Moreover, concentrations of TNF-α, IL-6 and IL-1β in ASC group obviously decreased (P<0.05). The expression of GLUT4, INSR, and phosphorylation of insulin signaling molecules in insulin target tissues were effectively improved. ASC infusion could aid in T2DM through recovery of islet β cells and improvement of insulin sensitivity. Autologous ASC infusion might be an effective method for T2DM.

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Section: A B Csupporting

confidence: 91%

Section: Isolation and Culture Of Ascmentioning

confidence: 99%

mentioning

confidence: 95%

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Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Chen

et al. 2015

Endocr J

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“…While ASCs have shown potential for improving the survival of both ischemic flaps and fat grafts, their ability for de novo adipogenesis represents an alternative means for restoration of contour deformities. [2][3][4] This has been demonstrated by Tsuji et al, who observed that ASCs seeded on a type I collagen sponge formed fat in vivo when implanted into immunocompromised mice. 5 As ASCs are a heterogeneous mesenchymal stem/progenitor cell population, enrichment for subpopulations with enhanced differentiation potential is a promising strategy for the development of targeted cell-based therapies.…”

Section: Introductionmentioning

confidence: 74%

Enrichment of Adipose-Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis

ZielinsElizabeth

PaikKevin

Ransom

et al. 2016

Tissue Engineering Part A

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“…Due to its own characteristics, ASC may be involved in the formation of tumor-associated matrix that has been shown to promote tumor cell metabolism and infiltration, and SVF promotes microvascular and collagen synthesis [25,26] . Thus, it is believed that ASC/SVF may promote tumor formation.…”

Section: Laboratory Studies Of Adsc and Breast Cancermentioning

confidence: 99%

Research progress on the safety of cell-assisted lipotransfer in breast repairs after breast conserving therapy

Liu¹,

Han²

2016

Adv Mod Oncol Res

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With the development of medical technology, more breast cancer patients have the opportunity of receiving breast conserving therapy. Post-operation breast reconstructions have gained more attention nowadays. As a new and important method of breast reconstruction after breast surgery, cell-assisted lipotransfer is being carried out in some clinical works. This paper briefly reviewed the current research progress of adipose-derived stem cell-assisted lipotransfer and the recurrence of breast cancers.

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Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial

Cited by 484 publications

References 28 publications

Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Enrichment of Adipose-Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis

Research progress on the safety of cell-assisted lipotransfer in breast repairs after breast conserving therapy

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