Enrichment of antibiotic resistance genes (ARGs) in polyaromatic hydrocarbon–contaminated soils: a major challenge for environmental health

Maurya, Anand Prakash; Rajkumari, Jina; Pandey, Piyush

doi:10.1007/s11356-020-12171-3

Cited by 23 publications

(13 citation statements)

References 72 publications

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“…In our study, POP tolerance was not correlated with antimicrobial activity as described previously [30]. In fact, the most resistant bacterial strains MONT-RG-20F-10-E-7-02, MONT-RG-20F-R14-05, TREG-RG-20F-10-E-5-01 and TREG-RG-20F-10-E-6-01 did not show a strong POP tolerance.…”

Section: Resultssupporting

confidence: 66%

“…We attempted to isolate antibiotic resistant bacteria, as Grube and collaborators identified in lichen-associated bacteria genes coding for multidrug resistance efflux pumps [11, 37]. Moreover, we used antibiotics because there are molecular evidences that the presence of POP can be implicated in the selection of microbes displaying antibiotic resistance genes in contaminated soils [30].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, three of the sampling locations had history of oil spills. As a previous study revealed that the interaction of mutagenic compounds in natural environments is correlated to the development of antibiotic resistance genes in the bacteria [30], we investigated the possible link between antibiotic resistance and tolerance to Persistent Organic Pollutants (POP). We used 9 culturing media mimicking the lichen habitat and two culturing methods to isolate the widest bacterial diversity.…”

Section: Introductionmentioning

confidence: 99%

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Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Miral

Kautsky

Alves-Carvalho

et al. 2022

Preprint

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As rock-inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifestyles and ecological plasticity, enabling them to withstand extreme environments. Because of their ability to grow on poor and extreme habitats, bacteria associated with lichens can tolerate a wide range of pollutants and on the other hand secrete antimicrobial compounds. In addition, lichen-associated bacteria were described to harbor ecological functions crucial for the evolution of the lichen holobiont. Nevertheless, the ecological features of lichen-associated microbes are still underestimated. To explore the untapped ecological diversity of lichen-associated bacteria, we adopted a novel culturomic approach on the crustose lichen Rhizocarpon geographicum. We sampled R. geographicum in French habitats exposed to oil spill and we combined 9 culturing methods with 16S rRNA sequencing to capture the largest bacterial diversity. A deep functional analysis of the lichen-associated bacterial collection showed the presence of a set of bacterial strains resistant to a wide range of antibiotics and displaying tolerance to Persistent Organic Pollutants (POP). Our study is a starting point to explore the ecological features of the lichen microbiota.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Miral

Kautsky

Alves-Carvalho

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…Numerous evidences have demonstrated that the propagation of ARGs and ARB in the environments could be promoted by selective factors including antibiotic (Ben et al 2019;Zhou et al 2022), disinfectant (Kampf 2018), pesticide (Ramakrishnan et al 2019), and heavy metals (Poole 2017). More recently, the influences of organic matters (especially polyaromatic hydrocarbons, PAHs) on the evolution of antibiotic resistance in the environments have attracted growing attention (Das et al 2021;Maurya et al 2021;Sun et al 2015;Tan et al 2019;Wang et al 2017a). A metagenomic study showed that the abundances of ARGs in the PAH-contaminated soils collected from a petrochemical plant were approximately 15 times higher than those in the background sites (Chen et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Insight into the impacts and mechanisms of ketone stress on the antibiotic resistance in Escherichia coli

Tang

Xiao

Gao

et al. 2022

Environ Sci Pollut Res

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Accumulation of toxic organic has posed a substantial pressure on the proliferation of bacterial resistance. While aromatic organics have been demonstrated to enhance the antibiotic resistance in bacteria, no information is yet available on the effects of non-aromatic organics on the variations of bacterial resistance. Here, we investigated the effects of a typical ketone (i.e., methylisobutanone (MIBK)) on the variations of antibiotic resistance in Escherichia coli (E. coli). The results showed that the growth of resistant E. coli under environmental concentration of 50 μg/L MIBK was firstly inhibited as explained by the transient disruption in the cell membrane and then recovered possibly due to the reactive oxygen species. Exposure to 50 μg/L MIBK gradually raised the abundance of representative resistance gene (ampR) in E. coli. In contrast, the high concentration of 50 mg/L MIBK continuously inhibited the growth of resistant E. coli by disrupting cell membrane and notably promoted the proliferation of ampR through enhancing the horizontal transformation and up-regulating the expression of efflux pump gene. These findings provided the first evidence for the evolution of bacterial resistance in response to ketone organics.

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“…Among the abiotic factors shaping the antibiotic resistome in environment, anthropogenic pollutants released by human activities are important contributors, which have attracted considerable interest in recent years . The effects of a list of environmental contaminants (e.g., organics, heavy metals, and nutrients − ) on the antibiotic resistome in various environmental compartments (e.g. soil, − anaerobic digester, sediment, , and biofilm reactor) have been explored, revealing an overall enrichment of the resistome and promotion of ARG dissemination due to selective pressure imposed by the contaminants.…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

Xiao

Jiang

et al. 2021

ACS Agric. Sci. Technol.

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Antibiotic resistance genes (ARGs) are emerging environmental pollutants, and the effect of both abiotic (e.g., anthropogenic pollutants such as sulfonamides (SAs)) and biotic (e.g., microbial community (taxonomy), mobile genetic elements (MGEs)) factors on the antibiotic resistome (collection of ARGs) has received much attention recently. In addition, function (e.g., CAZy, eggNOG and KEGG) is also an important biotic factor; however, how function correlates to ARGs and thus contributes to the antibiotic resistome remains ambiguous. This work explores the effect of sulfonamides (SAs) on ARGs and MGEs by a metagenomic analysis, a principal component analysis (PCA), and a nonmetric multidimensional scaling analysis (NMDS). Then, the work establishes a correlation between ARGs and function with a co-occurrence network and reveals the contribution and positive (negative)/direct (indirect) effect of taxonomy, MGEs, and function to the antibiotic resistome by variation partitioning analysis (VPA) and partial least-squares-path modeling (PLS-PM). The results show that the soil has diverse ARGs and several MGEs and SAs impose significant effects on them. Further, CAZy, eggNOG, and KEGG (including pathway and orthology) show strong and significant correlation with ARGs on the basis of the co-occurrence network; however, in our system it seems that only the KEGG pathway promotes the interpretation of ARGs and should be regarded as the priority function on considering the role of function in structuring the antibiotic resistome. This work reveals the importance and necessity of function as a biotic factor in shaping the antibiotic resistome in diverse resistome-related fields.

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Enrichment of antibiotic resistance genes (ARGs) in polyaromatic hydrocarbon–contaminated soils: a major challenge for environmental health

Cited by 23 publications

References 72 publications

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Insight into the impacts and mechanisms of ketone stress on the antibiotic resistance in Escherichia coli

Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

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