Enrichment, isolation and counting of soil microorganisms

Alef, Kassem

doi:10.1016/b978-012513840-6/50019-7

Cited by 42 publications

(34 citation statements)

References 95 publications

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“…All tests on solid media (10 % TSA), selective medium for Pseudomonas (Grant and Holt 1977) and Streptomyces (Bacto Malt-Extract Agar) were determined by the surface plating technique using 0.1 mL of appropriately diluted sample. After serial dilution in 0.85 % NaCl, dilutions were plated onto 10 % tryptic soy agar (TSA) on the selective medium for Pseudomonas and on Czapek-Dox medium with rose bengal for soil fungi (Alef 1995).…”

Section: Microbial Investigationmentioning

confidence: 99%

Ecological strategy for soil contaminated with mercury

et al. 2016

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Aims The paper presents results from plot experiments aimed at the development of an ecological strategy for soil contaminated with mercury. Meadow grass (Poa pratensis) was tested on mercury contaminated soil in a former chlor-alkali plant (CAP) in southern Poland for its phytoremediation potential. Methods The stabilisation potential of the plants was investigated on plots without additives and after the addition of granular sulphur. Biomass production, uptake and distribution of mercury by plants, as well as leachates and rhizosphere microorganisms were investigated, along with the growth and vitality of plants during one growing season. Results The analysed plants grew easily on mercury contaminated soil, accumulating lower amounts of mercury, especially in the roots, from soil with additive of granular sulphur (0.5 % w/w) and sustained a rich microbial population in the rhizosphere. After amendment application the reduction of Hg evaporation was observed.Conclusions The obtained results demonstrate the potential of using Poa pratensis and sulphur for remediation of mercury contaminated soil and reduction of the Hg evaporation from soil. In the presented study, methods of Hg reduction on Bhot spots^were proposed, with a special focus on environmental protection. This approach provides a simple remediation tool for large areas heavily contaminated with mercury.

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Section: Microbial Investigationmentioning

confidence: 99%

Ecological strategy for soil contaminated with mercury

et al. 2016

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“…Subsamples from each plot within a treatment were combined to produce a composite sample for assessing the population of fungi. Extraction of fungal propagules and dilution plating methods followed a modified method of Alef (1995). Moist soil (5 g) was mixed in 45 ml of 0.1% Na 2 H 2 P 2 O 7 for 1 h on an end-over-end shaker.…”

Section: Nontarget Fungimentioning

confidence: 99%

Arbuscular mycorrhizas influence plant diversity and community structure in a semiarid herbland

2002

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Summary• The contribution of arbuscular mycorrhizas (AM) to plant community structure and diversity is reported here in an annual herbland in southern Australia.• Mycorrhizal colonization was reduced in field plots by applying the fungicide benomyl as a soil drench. The mycorrhiza-responsiveness of plant species was assessed in intact soil cores containing the indigenous AM fungi and in a pot experiment using an isolate of Glomus mosseae .• Glasshouse experiments showed that Medicago minima, Vittadinia gracilis and Velleia arguta were highly mycorrhiza-responsive, Salvia verbenaca became colonized but exhibited no growth response to AM, and Carrichtera annua remained uncolonized. There was no change in plant species richness in mycorrhizasuppressed field plots but diversity increased owing to an increase in evenness. Treatment had no effect on community productivity and therefore there was no relationship between mycorrhizal effects on diversity and productivity.• Mycorrhizal responsiveness was not a good predictor of species response to suppression of AM in the field. The mycorrhiza-responsive species V. gracilis and V. arguta were not affected by reduced mycorrhizal colonization in fungicide-treated plots, suggesting that competition from the mycorrhiza-responsive dominant M. minima offset the benefits of mycorrhizal association for these species.

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“…Las bacterias aerobias y hongos filamentosos se determinaron mediante cuenta directa en placa, para lo cual se realizaron diluciones decimales de suelo (Alef, 1995). Las bacterias aerobias y actinomicetes se cuantificaron en los medios de cultivo agar glucosa-peptona, agar extracto de malta-rosa de bengala y agar caseína-peptona-almidón con extracto de levadura.…”

Section: Variables De Respuestaunclassified

“…Los resultados de la cuantificación de bacterias aerobias y hongos filamentosos se reportaron en UFC/g suelo. Para la cuantificación de esporas de hongos micorrizógenos se utilizó la metodología de flotación-centrifugación en solución de azúcar (Bagyaraj y Stürmer, 2008) protozoarios se determinaron mediante la técnica del número más probable (NMP) (Alef, 1995;Coleman et al, 2004), y para realizar los cálculos correspondientes se utilizó el programa 'MPN Calculator'. Los resultados se reportaron en NMP de protozoarios/g suelo.…”

Section: Variables De Respuestaunclassified

Efecto de dos abonos orgánicos en las cadenas tróficas del suelo cultivado con mora

Aceves¹,

Araya

Tabares

et al. 2017

Mesoam. Agron.

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Soil fertilization with organic fertilizers comprises a practice that improves the soil biological properties; however, the effect of these on the soil food web (SFW) has been scarcely studied. The aim of this study was to determine the effect of two commercial organic fertilizers on the structure of the SFW associated with roots of blackberry plants (Rubus adenotrichos). The research was conducted in two blackberry plantations located one in San Martín de León Cortés, and the other one in Buena Vista de Pérez Zeledón in San José, Costa Rica, from August to December, 2010. In the two plantations, plants were fertilized with compost or vermicompost. The roots of blackberry plants surrounding soil were sampled in order to quantify groups of the SFW through the following techniques: bacteria and filamentous fungi by plate count, protozoa by the most probable number, spores from arbuscular mycorrhizal fungi and nematodes by flotation-centrifugation, microarthropods, macroarthropods, and worms were directly counted in soil samples. The dataset was analyzed by multidimensional scaling analysis. The addition of organic fertilizers to soil caused a differential effect on the structure of the SFW (as compared with non-fertilized soils). The effect differed in soil from each of the experimental plantations according to fertilizer type. The groups of organisms mainly affected were actinomycetes and protozoa, which implies that the structure of SFW and consequently, the function of soil were not affected by the addition of organic fertilizers.

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Enrichment, isolation and counting of soil microorganisms

Cited by 42 publications

References 95 publications

Ecological strategy for soil contaminated with mercury

Ecological strategy for soil contaminated with mercury

Arbuscular mycorrhizas influence plant diversity and community structure in a semiarid herbland

Efecto de dos abonos orgánicos en las cadenas tróficas del suelo cultivado con mora

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