Enrichment culture and molecular identification of diverse Bartonella species in stray dogs

Bai, Ying; Kosoy, Michael; Boonmar, Sumalee; Sawatwong, Pongpun; Sangmaneedet, Somboon; Peruski, Leonard F.

doi:10.1016/j.vetmic.2010.05.017

Cited by 51 publications

(57 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some specimens may benefit from pre-enrichment in liquid growth medium before extraction Duncan et al 2007;Riess et al 2008;Bai et al 2010) or extended lysis steps during the extraction process. Gutiérrez et al (2017) provide an excellent review of recommended protocols for homogenization and extraction.…”

Section: Recommendations For the Genotyping Of Bartonella Speciesmentioning

confidence: 99%

Genotyping ofBartonellabacteria and their animal hosts: current status and perspectives

et al. 2017

View full text Add to dashboard Cite

S U M M A R YGrowing evidence demonstrates that bacterial species diversity is substantial, and many of these species are pathogenic in some contexts or hosts. At the same time, laboratories and museums have collected valuable animal tissue and ectoparasite samples that may contain substantial novel information on bacterial prevalence and diversity. However, the identification of bacterial species is challenging, partly due to the difficulty in culturing many microbes and the reliance on molecular data. Although the genomics revolution will surely add to our knowledge of bacterial systematics, these approaches are not accessible to all researchers and rely predominantly on cultured isolates. Thus, there is a need for comprehensive molecular analyses capable of accurately genotyping bacteria from animal tissues or ectoparasites using common methods that will facilitate large-scale comparisons of species diversity and prevalence. To illustrate the challenges of genotyping bacteria, we focus on the genus Bartonella, vector-borne bacteria common in mammals. We highlight the value and limitations of commonly used techniques for genotyping bartonellae and make recommendations for researchers interested in studying the diversity of these bacteria in various samples. Our recommendations could be applicable to many bacterial taxa (with some modifications) and could lead to a more complete understanding of bacterial species diversity.

show abstract

Section: Recommendations For the Genotyping Of Bartonella Speciesmentioning

confidence: 99%

Genotyping ofBartonellabacteria and their animal hosts: current status and perspectives

et al. 2017

View full text Add to dashboard Cite

show abstract

“…arupensis, B. elizabethae, B. grahamii, B. quintana, B. taylorii, and novel Bartonella genotypes have been found in dogs. 11,16 In rodent species, B. grahamii, B. elizabethae, Candidatus Bartonella thailandensis, B. coopersplainensis, B. phoceensis, B. rattimassiliensis, B. tribocorum, and novel Bartonella genotypes have been detected by culture and polymerase chain reaction (PCR) analysis. 12,13,17 However, little information has been obtained to identify potential arthropod vectors of Bartonella species in Thailand.…”

mentioning

confidence: 99%

Molecular Detection and Identification of Bartonella Species in Rat Fleas from Northeastern Thailand

Billeter¹,

Colton²,

Sangmaneedet³

et al. 2013

The American Society of Tropical Medicine and Hygiene

Self Cite

View full text Add to dashboard Cite

Abstract. The presence of Bartonella species in Xenopsylla cheopis fleas collected from Rattus spp. (R. exulans, R. norvegicus, and R. rattus) in Khon Kaen Province, Thailand was investigated. One hundred ninety-three fleas obtained from 62 rats, were screened by polymerase chain reaction using primers specific for the 16S-23S intergenic spacer region, and the presence of Bartonella DNA was confirmed by using the citrate synthase gene. Bartonella DNA was detected in 59.1% (114 of 193) of fleas examined. Sequencing demonstrated the presence of Bartonella spp. similar to B. elizabethae, B. rattimassiliensis, B. rochalimae, and B. tribocorum in the samples tested with a cutoff for sequence similarity 96% and 4 clustered together with the closest match with B. grahamii (95.5% identity). If X. cheopis proves to be a competent vector of these species, our results suggest that humans and animals residing in this area may be at risk for infection by several zoonotic Bartonella species.

show abstract

“…Numerous Bartonella species have been associated with clinical illnesses ranging from mild skin lesions to more severe manifestations, including persistent fevers, neurological symptoms, and endocarditis (6,10). These bacteria can be fastidious under current laboratory conditions, and attempts to isolate cells from pure culture of biological specimens are often unsuccessful, despite positive molecular detection (1,7).…”

mentioning

confidence: 99%

Combining Culture Techniques for Bartonella: the Best of Both Worlds

2011

View full text Add to dashboard Cite

In this study we compared some common Bartonella culturing methodologies using four diverse species causing human illnesses. Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that Bartonella tamiae demonstrated temperature-dependent growth limitations between common culturing conditions only 2°C apart. Additionally, growth of B. quintana was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature-and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.Bartonella is a genus of Gram-negative, facultative intracellular bacteria that have been detected in a plethora of insect and mammalian hosts (5,14,19,25). Numerous Bartonella species have been associated with clinical illnesses ranging from mild skin lesions to more severe manifestations, including persistent fevers, neurological symptoms, and endocarditis (6, 10). These bacteria can be fastidious under current laboratory conditions, and attempts to isolate cells from pure culture of biological specimens are often unsuccessful, despite positive molecular detection (1, 7).The majority of culturing methods for Bartonella species found in the literature focus on growth requirements for the two species commonly associated with human infection, Bartonella henselae and B. quintana (3,9,22,27,35). Through a review of the literature on methods for Bartonella isolation from biological samples, we found many differences in the culturing protocols. To summarize, most laboratories use cocultivation with mammalian cells or an axenic, insect cell culture-based medium or plating onto blood-supplemented agar (15,24). The mammalian cell coculture and insect cell culturebased medium are usually used for enrichment before plating of samples on agar. Of particular interest is the variability between the cell culture systems, as this method is traditionally considered to be the most successful for the initial isolation of Bartonella species (22,23,27,31). We focused our comparison on the protocols of three prominent laboratories that commonly culture Bartonella spp. and found the major differences to be localized to three main variables: (i) medium base (RPMI, medium 199 [M199], or Dulbecco modified Eagle medium [DMEM]), (ii) mammalian cell line (bovine endothelial, human endothelial, primate epithelial), and (iii) culturing temperature (35°C, 37°C).In 2005, a cell-free, liquid medium called Bartonella alphaproteobacteria growth medium (BAPGM) was developed to detect Bartonella in veterinary and human blood samples (28). It is a modified formulation of liquid medium designed to support insect cells. This medium required sheep ...

show abstract

Enrichment culture and molecular identification of diverse Bartonella species in stray dogs

Cited by 51 publications

References 28 publications

Genotyping ofBartonellabacteria and their animal hosts: current status and perspectives

Genotyping ofBartonellabacteria and their animal hosts: current status and perspectives

Molecular Detection and Identification of Bartonella Species in Rat Fleas from Northeastern Thailand

Combining Culture Techniques for Bartonella: the Best of Both Worlds

Contact Info

Product

Resources

About