Enrichment behavior of immunoglobulin by foam fractionation using response surface methodology

Chen, Yen-Chih; Parlar, Harun

doi:10.1016/j.seppur.2013.01.026

Cited by 19 publications

(6 citation statements)

References 66 publications

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“…Thus the amount of nisin adsorbed at the bubble surfaces was high, and thus the nisin recovery was improved. These results were in agreement with previous reports …”

Section: Resultssupporting

confidence: 94%

Study on the process of fermentation coupling with foam fractionation and membrane module for nisin production

Cui

Zhang

Zheng

et al. 2014

Asia-Pacific J Chem Eng

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Nisin, an antimicrobial peptide, is commercially used as a food preservative and a promising alternative in medical applications for inhibiting bacterial infection in both humans and livestock. It is produced by fermentation using certain strains of Lactococcus lactis ssp. lactis. In traditional fermentation, the yield of nisin has always been limited by adsorption of nisin onto its producer, enzymatic degradation and product inhibition. This limitation can be solved by fast removal of the product from the broth during fermentation. In the current work, a technology of fermentation coupling with foam fractionation and membrane module was developed to achieve in situ removal of nisin produced by L. lactis ssp. lactis ATCC 11454 from the broth. The effects of air flow rate, the initial aeration time and interval time on the performance of the technology were studied to increase the yield of nisin. The results showed that under the optimal conditions of air flow rate 60 mL/min, the initial aeration time of the fourth hour and regular 30-min interval, the total nisin titer reached 3946 IU/mL, increased by 24.2%, compared with the control group (3177 IU/mL). Therefore the coupling technology can effectively weaken the feedback inhibition and thus increase the yield of nisin by in situ fast removal of the product from broth during fermentation.

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“…Thus the amount of nisin adsorbed at the bubble surfaces was high, and thus the nisin recovery was improved. These results were in agreement with previous reports …”

Section: Resultssupporting

confidence: 94%

Study on the process of fermentation coupling with foam fractionation and membrane module for nisin production

Cui

Zhang

Zheng

et al. 2014

Asia-Pacific J Chem Eng

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“…and possible interactions between the independent variables and their effects on the separation performance of a Knelson Concentrator. CCD, a well-suited RSM for fitting a secondorder response surface, was used to design the experiments (Box and Hunter, 1957;Box and Wilson, 1992;Chen and Parlar, 2013;Montgomery, 2009;Yi et al, 2010). Each variable has five levels (±β, ±1, 0 where β = 2 3/4 = 1.682), with grade and recovery of magnetite chosen as the responses.…”

Section: Methodsmentioning

confidence: 99%

A design of experiments investigation into the processing of fine low specific gravity minerals using a laboratory Knelson Concentrator

Marion

Langlois

Kökkılıç

et al. 2019

Minerals Engineering

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Where a licence is displayed above, please note the terms and conditions of the licence govern your use of this document. When citing, please reference the published version. Take down policy While the University of Birmingham exercises care and attention in making items available there are rare occasions when an item has been uploaded in error or has been deemed to be commercially or otherwise sensitive.

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“…As a result, VSTs effectively improved R. However, the enhancement of protein adsorption also reduced bubble size in foam, and hence increased liquid holdup in foam [27]. Because it had a similar level to the increase of liquid holdup In foam fractionation, decreasing initial feed concentration often increases enrichment ratio but reduces recovery yield [29]. Thus, to select a suitable initial feed concentration (C 0 ) of total anthraquinones for the two-stage foam fractionation technology, its effects on E, R, and C f were investigated using the foam fractionation column with VSTs.…”

Section: Effects Of Vsts On E and Rmentioning

confidence: 99%

Separation of Protein-Binding Anthraquinones from Semen Cassiae Using Two-Stage Foam Fractionation

Ding

Wang²,

Yue

et al. 2019

Processes

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Anthraquinones are compounds of high medicinal value in many plants. Based on their good protein binding affinity, foam fractionation was attempted to separate them using proteins in the aqueous extract of Semen Cassiae as collectors. Firstly, the interaction between anthraquinones and Semen Cassiae proteins has been analyzed by the Stem–Volmer equation with physcion as a standard. The results show that physcion had good interaction with the proteins via hydrophobic forces. More importantly, the proteins effectively assisted the foam fractionation of several anthraquinones including aurantio-obtusifolin, aloe-emodin, rhein, emodin, chrysophanol, and physcion. On this basis, a two-stage foam fractionation technology was developed for process intensification using a foam fractionation with vertical sieve trays (VSTs). VSTs, initial feed concentration of total anthraquinones, temperature, volumetric air flow rate and pore diameter of gas distributor had significant effects on enrichment ratio and recovery yield of anthraquinones. Under suitable conditions, the enrichment ratio of total anthraquinones reached 47.0 ± 4.5 with a concentration of 939 ± 94 mg/L in the foamate while their total recovery percentage reached more than 47.7%. In addition, foam fractionation also increased the purity and hydroxyl radical scavenging activity of total anthraquinones. The results had significant implications for the separation of anthraquinones from Semen Cassiae.

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Enrichment behavior of immunoglobulin by foam fractionation using response surface methodology

Cited by 19 publications

References 66 publications

Study on the process of fermentation coupling with foam fractionation and membrane module for nisin production

Study on the process of fermentation coupling with foam fractionation and membrane module for nisin production

A design of experiments investigation into the processing of fine low specific gravity minerals using a laboratory Knelson Concentrator

Separation of Protein-Binding Anthraquinones from Semen Cassiae Using Two-Stage Foam Fractionation

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