Enrichment and single-cell analysis of circulating tumor cells

Song, Yanling; Tian, Tian; Shi, Yuanzhi; Liu, Wenli; Zou, Yu; Khajvand, Tahereh; Wang, Sili; Zhu, Zhi; Yang, Chaoyong

doi:10.1039/c6sc04671a

Cited by 150 publications

(122 citation statements)

References 109 publications

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“…Circulating tumor cell analysis can provide rich information for disease diagnosis and therapy assessment in anon-invasive manner. [1] However,t he extraordinary rarity (as few as several CTCs among 10 9 blood cells) makes their isolation challenging.M any enrichment platforms have been developed, [2] including filtration, [3] dielectrophoresis, [4] gradient centrifugation, [5] spiral microfluidics, [6] and affinity-based capture. [7] One of the important trends is to facilitate more in-depth characterization beyond enumeration.…”

mentioning

confidence: 99%

Bioinspired Engineering of a Multivalent Aptamer‐Functionalized Nanointerface to Enhance the Capture and Release of Circulating Tumor Cells

Song,

Shi,

Huang

et al. 2019

Angew Chem Int Ed

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Circulating tumor cell (CTC)-enrichment by using aptamers has an umber of advantages,b ut the issue of compromised binding affinities and stabilities in real samples hinders its wide applications.Inspired by the high efficiency of the prey mechanism of the octopus,weengineered adeterministic lateral displacement (DLD)-patterned microfluidic chip modified with multivalent aptamer-functionalized nanospheres (AP-Octopus-Chip) to enhance capture efficiency.T he multivalent aptamer-antigen binding efficiency improves 100-fold and the capture efficiency is enhanced more than 300 % compared with am onovalent aptamer-modified chip.M oreover,t he captured cancer cells can be released through athiol exchange reaction with up to 80 %e fficiency and 96 % viability,whichisfully compatible with downstream mutation detection and CTC culture.Using the chip,wewere able to find CTCs in all cancer samples analyzed.

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mentioning

confidence: 99%

Bioinspired Engineering of a Multivalent Aptamer‐Functionalized Nanointerface to Enhance the Capture and Release of Circulating Tumor Cells

Song,

Shi,

Huang

et al. 2019

Angew Chem Int Ed

Self Cite

183

201

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“…This IMPACT chip concept can be extended to detect many biomarkers such as exosomes 43 , single cells 44 , and surface proteins 45 . For example, the detection sensitivity of traditional immunoassay approaches is limited due to the low binding capacity.…”

Section: Discussionmentioning

confidence: 99%

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Bao

et al. 2020

Preprint

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A fully Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) system is developed for viral DNA detection. This powerful system is patterned with high-aspect ratio micropillars to enhance reporter probe binding. After surface modification and probe immobilization, CRISPR Cas12a/crRNA complex is injected into the fully enclosed system. With the presence of double-stranded DNA target, the CRISPR enzyme is activated and non-specifically cleaves the ssDNA reporters initially immobilized on the micropillars. This collateral cleavage releases fluorescence dyes into the assay, and the intensity is linearly proportional to the target DNA concentration ranging from 0.1 to 10 nM. Importantly, this system does not rely on traditional dye-quencher labeled probe thus eliminating the fluorescence background presented in the assay. Furthermore, our one-step detection protocol is performed at isothermal conditions (37°C) without using complicated and time-consuming off-chip probe hybridization and denaturation. This miniaturized and fully packed IMPACT chip demonstrates rapid, sensitive, and simple nucleic acid detection and is an ideal candidate for the next generation molecular diagnostic platform for point-of-care (POC) applications, responding to emerging and deadly pathogen outbreaks.

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“…CTCs have been shown to be of prognostic significance in patients with various cancers 2, 15, 34 . It is suspected that a large number of CTCs do not portray the signature of cancer epithelium, largely due to their acquired phenotype that is suitable for migration 34 .…”

Section: Discussionmentioning

confidence: 99%

“…A staggering 90% of cancer deaths are attributable to metastases 1 . After detaching from solid tumors, cancer cells travel through the bloodstream to reach distant organs and seed the development of metastatic tumors 2 . Cancer cells under circulation are called circulating tumor cells (CTCs) 3 .…”

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confidence: 99%

Integrative analysis and machine learning based characterization of single circulating tumor cells

Iyer

Gupta

Sharma

et al. 2019

Preprint

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ABSTRACTWe collated publicly available single-cell expression profiles of circulating tumor cells (CTCs) and showed that CTCs across cancers lie on a near-perfect continuum of epithelial to mesenchymal (EMT) transition. Integrative analysis of CTC transcriptomes also highlighted the inverse gene expression pattern between PD-L1 and MHC, which is implicated in cancer immunotherapy. We used the CTCs expression profiles in tandem with publicly available peripheral blood mononuclear cell (PBMC) transcriptomes to train a classifier that accurately recognizes CTCs of diverse phenotype. Further, we used this classifier to validate circulating breast tumor cells captured using a newly developed microfluidic systems for label-free enrichment of CTCs.

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Enrichment and single-cell analysis of circulating tumor cells

Abstract: This review aims to provide in-depth insights into CTC analysis, including various techniques for isolation of CTCs and single-cell analysis of CTCs, as well as current developmental trends and promising research directions.

Cited by 150 publications

References 109 publications

Bioinspired Engineering of a Multivalent Aptamer‐Functionalized Nanointerface to Enhance the Capture and Release of Circulating Tumor Cells

Bioinspired Engineering of a Multivalent Aptamer‐Functionalized Nanointerface to Enhance the Capture and Release of Circulating Tumor Cells

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Integrative analysis and machine learning based characterization of single circulating tumor cells

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