Enrichment and purification of total flavonoids from Cortex Juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in Mice

Zhao, Peng; Qi, Chao; Wang, Gang; Dai, Xinpeng; Hou, Xiaohong

doi:10.1016/j.jchromb.2015.10.019

Cited by 34 publications

(13 citation statements)

References 34 publications

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“…Carbon tetrachloride (CCl 4 ) has been extensively used in animal models to explore hepatic injury and to investigate hypoprotective effect of candidates [ 14 ]. Many herb medicines or natural products have been proven to exhibit hypoprotective effect on CCl 4 induced acute liver injury models [ 15 , 16 ]. In the present work, 1 H-NMR based metabolomics combined with conventional serum chemistry analysis as well as tissue section examination were carried out to investigate the protective effect of QJ (1,2 g/kg) on acute hepatic damaged rats induced by CCl 4 .…”

Section: Introductionmentioning

confidence: 99%

¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Gentiana macrophylla Radix, commonly known as Qin-Jiao (QJ), was recorded alone to treat jaundice in Compendium of Materia Medica and has been frequently prescribed for treatment of liver disease in China. However, the underlying mechanism remains unknown. In the present work, QJ of 1,2 g/kg or silybin of 40 mg/kg (positive control) was orally given to rats for 7 days to verify the protective effect on acute liver damage induced by tetrachloride (CCl4). Together with serum biochemistry and histopathological examination, 1H-NMR based metabolomics work was carried out to investigate the efficacy. It turned out that QJ of 2 g/kg exerted comparable protective effect with positive control and partially recovered disturbed metabolism by CCl4. Multivariate analysis was conducted and metabolites altered significantly among groups were assigned and discussed, including betaine, glucose, lactate, creatine, and LDL/VLDL. Metabolic regulations involved in QJ or silybin treatment were as follows: tricarboxylic acid (TCA) cycle, synthesis of LDL/VLDL, and gluconeogenesis were enhanced, while betaine metabolism, glycolysis, creatine metabolism, synthesis of ketone bodies, amino acids metabolism, and β-oxidation of fatty acids were suppressed. For the first time hepatoprotective effect of QJ on acute liver damage was revealed by 1H-NMR based metabolomics, prompting understanding of the underlying mechanism.

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Section: Introductionmentioning

confidence: 99%

¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Macroporous resin adsorption method with multiple advantages of removing impurities, high adsorption capacity and repeated utilization during the purification process of natural products has been gradually recognized and applied [14][15][16][17]. Many experiments show that macroporous resin has excellent separation efficiency for flavonoids [18][19][20]. The purification and enrichment of flavonoids through macroporous resin can provide a basis for the further exploitation and utilization of loquat leaves.…”

Section: Introductionmentioning

confidence: 99%

Purification of total flavonoids from loquat leaves by macroporous resin and corresponding antioxidant capacity

Huang

Lin

et al. 2017

BIO Web Conf.

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Flavonoids is one of the major active compounds in loquat leaves. In this study, the purification process of total flavonoids in loquat leaves by macroporous resin was researched and the antioxidant activity of total flavonoids was determined. The active compounds were extracted by 95% ethanol, and the total flavonoids was purified by macroporous resin. Comparing the static and dynamic adsorption and desorption characters of 6 macroporous resin, the best type of macroporous resin was determined. The desorption-solvent was determined by the gradient elution. Moreover, DPPH and FRAP analysis methods were used to determine the antioxidant activity of total flavonoids in this study. The result showed that X-5 was the optimum macroporous resin to purify the total flavonoids of loquat leaf. After adsorbed by X-5 macroporous resin, removing impurity with 15% ethanol and desorbed with 65% ethanol, the total flavonoids purity of the eluate was 82.20%, the purification factor was 2.14 times and the recovery was 81.08%, and the macroporous resin should be regeneration after using 3 times. As the flavonoids content of extraction increase, its antioxidant activity increase. In summary, the X-5 macroporous resin was suitable to purify the total flavonoids of loquat for higher purification fold and higher recovery. Purified extract had higher content of flavonoid and better antioxidant activity, it suggested the flavonoids played an important role in antioxidant activity.

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“…A key parameter affecting the adsorption and desorption capacity of the resins is the pH value of the initial sample solutions. The pH value influences the extent of ionization of solute molecules and therefore affects the affinity between the solutes and solutions [ 19 ]. As shown in Figure 4 , for AB-8 resin, the adsorption capacity for the TGFs reached its crest value (141.57 mg/g) at pH 5.0, and then decreased gradually with the rise in pH value.…”

Section: Resultsmentioning

confidence: 99%

Enrichment and Purification of Total Ginkgo Flavonoid O-Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro

Zhang

Xiao

et al. 2018

Molecules

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In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total ginkgo flavonoid O-glycosides (TGFs) (quercetin (I), kaempferol (II), isorhamnetin (III)) from Ginkgo Biloba extracts (EGB) are evaluated. The adsorption and desorption properties of TGFs are studied on macroporous resins, including D101, D201, AB-8, HPD400, D301, and D311. Along with the results, AB-8 resin exhibits the best adsorption and desorption capacity for these three ginkgo flavonoid O-glycosides among the six resins. Adsorption isotherms are created on AB-8 resin and fit well to the Langmuir (R2 > 0.96) and Freundlich (R2 > 0.92, 0.3 < 1/n < 0.7) models. After the treatment with gradient elution on AB-8 resin packed chromatography column, the contents of the three main ginkgo flavonoid O-glycosides (I, II, and III) increase from 8.93%, 9.88%, and 6.11% in the extracts to 30.12%, 35.21%, and 14.14%, respectively, in the product. The recoveries of compounds I, II, and III are 88.76%, 93.78%, and 60.90%, respectively. Additionally, the anti-inflammatory effects of TGFs are evaluated in LPS-treated RAW 264.7 macrophages, and the result demonstrates that TGFs could significantly inhibit LPS-induced NO release in vitro in a dose-dependent manner compared with the control group. These findings suggest that TGFs could potentially be natural antioxidants and anti-inflammatory ingredients that could be used in pharmaceutical products and functional food additives.

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Enrichment and purification of total flavonoids from Cortex Juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in Mice

Cited by 34 publications

References 34 publications

¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

Purification of total flavonoids from loquat leaves by macroporous resin and corresponding antioxidant capacity

Enrichment and Purification of Total Ginkgo Flavonoid O-Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro

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Enrichment and purification of total flavonoids from Cortex Juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in Mice

Cited by 34 publications

References 34 publications

1H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

1H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

Purification of total flavonoids from loquat leaves by macroporous resin and corresponding antioxidant capacity

Enrichment and Purification of Total Ginkgo Flavonoid O-Glycosides from Ginkgo Biloba Extract with Macroporous Resin and Evaluation of Anti-Inflammation Activities In Vitro

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¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats

¹H‐NMR Based Serum Metabolomics Study to Investigate Hepatoprotective Effect of Qin‐Jiao on Carbon Tetrachloride‐Induced Acute Hepatotoxicity in Rats