Enrichment and Plating Methodology for Salmonella Detection in Food. A Review1

Fagerberg, D. J.; Avens, John S.

doi:10.4315/0022-2747-39.9.628

Cited by 39 publications

(23 citation statements)

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“…Salmonella strains that were not isolated on XLD-N and HE-N, but were isolated on XLD and HE, were subsequently plated on the media with N. The results indicate that N was not inhibitory for any of the Salmonella strains found in this investigation (data not presented). Sulfadiazine was added to BG agar to suppress the growth of Pseudomonas and Proteus (Fagerberg and Ayers 1976). Of the 40 Salmonella-positive samples identified, 65% were isolated on BGS medium.…”

Section: Isolation Efficacies Of Salmonellae On Various Plating Mediasupporting

confidence: 72%

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

Komatsu

Restaino

1981

Journal of Food Safety

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Five plating media, Hektoen enteric (HE) and xylose lysine deoxycholate (XLD) agars with and without 80 and 5 μg/ml of novobiocin (N), respectively, and brilliant greeen sulfadiazine (BGS) agar with 80 μg/ml of the antimicrobial agent, were analyzed for the recovery of salmonellae from various fresh beef, pork, and poultry meat products. Of the total Samonella positive samples, 50.0% and 82.5% were found on XLD and XLD‐N agars, respectively, 75.0% and 85.0% on HE and HE‐N agars, respectively and 65.0% on BGS agar. HE‐N and BGS media isolated three times more false positives than did XLD‐N agar, while XLD and HE agars gave the highest numbers of false positives. The major H2S producing false positive on XLD and HE agars was Proteus mirabilis. With the addition of N, P. mirabilis was eliminated, and the major H2S producing false positive was almost exclusively Citrobacter freundii. The false positives on BGS agar were predominately distributed among C. freundii, Enterobacter sp., and Klebsiella sp.

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Section: Isolation Efficacies Of Salmonellae On Various Plating Mediasupporting

confidence: 72%

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

Komatsu

Restaino

1981

Journal of Food Safety

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“…worthington, and that detection of the minor serotypes using this latter medium was more dif-ficult. Fagerberg and Avens (1976) reported that certain serotypes were easier to detect in certain media than in others.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Arroyo¹,

Arroyo

1995

Journal of Applied Bacteriology

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Rapid detection systems for Salmonella in foodstuffs are currently being developed. However, existing standards still call for application of traditional methods employing pre-enrichment followed by selective enrichment and isolation. The efficacy of various methods was tested using 264 chicken and lamb organ meats. Pre-enrichment was carried out in Tryptone Soy Broth (TSB) and enrichment in Tetrathionate Brilliant Green Broth (TTB) at 37 degrees C, Selenite Broth with Brilliant Green and Sulphapyridine at 37 degrees C and 43 degrees C, and Rappaport-Vassiliadis Broth (RV 10) at 42 degrees C. The isolation media were Brilliant Green Agar (BGA), Deoxycholate Citrate Agar, Hektoen Enteric Agar (HEA) and Salmonella-Shigella Agar. Enrichment in RV/42 degrees C followed by isolation on BGA as recommended by ISO standard no. 6579 and enrichment in TTB/37 degrees C followed by isolation in HEA, no longer recommended by that standard, produced the best results. Low percentages of positive samples and difficulties in detecting Salmonella are the result of interference by competing organisms (Enterobacteriaceae) and the number of salmonellas present after enrichment. A total of 528 samples (TSB, eggs, lamb liver and chicken liver) were inoculated with Salm. enteritidis, Salm. kapemba and Salm. virchow, and the preceding experiment was repeated. All the TSB and egg samples tested positive, but the percentage of positive samples from the lamb and chicken liver was only 81-92%. Recovery of the salmonellas did not depend upon the method employed or the serotype inoculated but instead on interference by competing flora and the numbers of Salmonella present in the samples.

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“…This can be explained by the microbiota found in these foods. In the 70's, Fagerberg and Avens (8) reported that SC was better for certain serotypes of Salmonella, while TT was more efficient for others. However, Cox and Mercuri (7) observed that TT was more toxic when the concentration of microorganisms was low.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

Rall¹,

Rall²,

Aragon³

et al. 2005

Braz. J. Microbiol.

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We evaluated the effectiveness of Selenite Cystine (SC), Tetrathionate Brilliant Green (TBG) and Rappaport Vassiliadis (RV) broths for Salmonella isolation. We also tested three classic plating media, SalmonellaShigella Agar (SS), Brilliant Green Agar (BGA), Xylose Lysine Desoxycholate Agar (XLD) and two chromogenic agars, Rambach (RA) and CHROMagar Salmonella (CAS). Among 100 poultry carcasses, 29 were positive for Salmonella using all plating media combined. RV broth (69%) and TT broth (58.6%) were more effective than SC broth (24.1%). The chromogenic media gave better results than the classic ones with less false-positive colonies. The most effective isolation medium was CHROMagar, where Salmonella was identified in 23 (79.3%) of the 29 positive samples, followed by Rambach (48%). Positivity for Salmonella using classic media was 13.8% for BGA, 27.6% for SS and 34.5% for XLD.

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Enrichment and Plating Methodology for Salmonella Detection in Food. A Review1

Cited by 39 publications

References 0 publications

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS

Efficiency of different enrichment and isolation procedures for the detection of Salmonella serotypes in edible offal

Evaluation of three enrichment broths and five plating media for Salmonella detection in poultry

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