Enrichment and fractionation of major apple flavonoids, phenolic acids and dihydrochalcones using anion exchange resins

Kammerer, Judith; Boschet, Jennifer; Kammerer, Dietmar R.; Carle, Reinhold

doi:10.1016/j.lwt.2010.10.008

Cited by 44 publications

(34 citation statements)

References 17 publications

(20 reference statements)

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“…This adaption was inevitable, even though knowing that such a modification is a change of a crucial parameter (Bretag et al. , 2009a,b; Kammerer et al. , 2010c, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, proteins, which were also present in the extracts as monitored by SDS-PAGE (data not shown), may also competitively bind to the sorbents (Weisz et al, 2010). This adaption was inevitable, even though knowing that such a modification is a change of a crucial parameter (Bretag et al, 2009a,b;Kammerer et al, 2010cKammerer et al, , 2011. Compared with the binding rates determined in the model experiments, the adsorption and ion exchange rates of the apple extract trials were comparable (Table 3 and Figure S1).…”

Section: Evaluation Of Polyphenol Sorption and Ion Exchange From Extrmentioning

confidence: 98%

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Recovery and fractionation of major apple and grape polyphenols from model solutions and crude plant extracts using ion exchange and adsorbent resins

Kammerer

Schweizer

Carle

2011

Int J of Food Sci Tech

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Model systems composed of phenolic compounds, amino acids and mixtures thereof mimicking apple pomace and grape skin extracts were used to examine adsorption and ion exchange behaviour of one adsorbent as well as four anion and four cation exchange resins in a column system at pH 1, 5 and 7. Furthermore, apple pomace and grape skin extracts were loaded onto the resins to evaluate the transferability of the results obtained in the model experiments. In addition, different eluents were applied for the successive recovery of phenolic compounds from the resins. The results provide valuable information on highly complex adsorption and exchange systems. The data indicate both enhanced and decreased polyphenol binding rates as a result of amino acid addition depending on the composition of the solutions applied to the resins. The application of appropriate process parameters during adsorption and desorption was shown to allow selective recovery of compound classes or even individual compounds. As the model experiments did not allow complete prediction of polyphenol binding behaviour, further extract compounds not investigated in the present study presumably also interfere with polyphenol recovery. The results help to systematically optimise industrial processes, thus improving cost-efficiency and selectivity of polyphenol recovery.

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“…This adaption was inevitable, even though knowing that such a modification is a change of a crucial parameter (Bretag et al. , 2009a,b; Kammerer et al. , 2010c, 2011).…”

Section: Resultsmentioning

confidence: 99%

Section: Evaluation Of Polyphenol Sorption and Ion Exchange From Extrmentioning

confidence: 98%

Recovery and fractionation of major apple and grape polyphenols from model solutions and crude plant extracts using ion exchange and adsorbent resins

Kammerer

Schweizer

Carle

2011

Int J of Food Sci Tech

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“…Increasing applications of resins are found in scientific literature for the recovery and non-thermal concentration and fractionation of the crude phenolic extracts from products and byproducts of the food industry, i.e., citrus peel and molasses [5], apple and grape pomace [6][7][8][9] or for solvent extracts from autohydrolysis liquors of grape pomace [10].…”

Section: Introductionmentioning

confidence: 99%

Recovery and Concentration of Antioxidants from Winery Wastes

et al. 2012

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Grape and wine byproducts have been extensively studied for the recovery of phenolic compounds with antioxidant activity and a variety of biological actions. The selective recovery and concentration of the phenolic compounds from the liquid phase separated from further diluted winery wastes has been proposed. Adsorption onto non ionic polymeric resins and further desorption with ethanolic solutions was studied. Several commercial food grade resins were screened with the aim of selecting the most suited for the practical recovery of phenolic compounds with radical scavenging activity. Under the optimized desorption conditions (using Sepabeads SP207 or Diaion HP20 as adsorbents and eluting with 96% ethanol at 50 °C) a powdered yellow-light brown product with 50% phenolic content, expressed as gallic acid equivalents, was obtained. The radical scavenging capacity of one gram of product was equivalent to 2-3 g of Trolox.

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“…Simultaneously, the same of the aqueous extract were filtered with 0–90% microporous resin, and collected 40–60% section. This section was re-melted with 30–50% methanol-water (50–100 mL), and standed for one night, then recrystallized respectively to get 5g phloridzin and trilobatin respectively [25]. Of course, the purity and structure of phloridzin and trilobatin were detected with 400M Hz 1 H NMR (Varian, US).…”

Section: Methodsmentioning

confidence: 99%

Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice

Wang

Huang

et al. 2016

PLoS ONE

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The purpose of this study was to investigate the effects of leaf extract from Lithocarpus polystachyus Rehd. on type II diabetes mellitus (T2DM) and the active ingredients of this effect. In addition, this study determined, for the first time, the underlying molecular and pharmacological mechanisms of the extracts on hyperglycemia using long-term double high diet-fed and streptozotocin (STZ) induced type II diabetic mice. In the present study, leaf extract, phloridzin and trilobatin were assessed in vivo (gavage) and in vitro (non-invasive micro-test technique, NMT) in experimental T2DM mice. The biochemical parameters were measured including blood glucose and blood lipid level, liver biochemical indexes, and hepatic glycogen. The relative expression of glycometabolism-related genes was detected. The effect of leaf extracts on physiological glucose flux in liver tissue from control and T2DM mice was also investigated. Body weight of experimental T2DM mice increased significantly after the first week, but stabilized over the subsequent three weeks; body weight of all other groups did not change during the four weeks’ study. After four weeks, all treatment groups decreased blood glucose, and treatment with leaf extract had numerous positive effects: a) promoted in glucose uptake in liver, b) increased synthesis of liver glycogen, c) reduced oxidative stress, d) up-regulation of glucokinase (GK), glucose transporter 2 (GLUT2), insulin receptor (IR) and insulin receptor substrate (IRS) expression in liver, e) down-regulation of glucose-6-phosphatase (G-6-P) expression, and f) ameliorated blood lipid levels. Both treatment with trilobatin or phloridzin accelerated liver glycogen synthesis, decreased oxidative stress and increased expression of GK. IRS and phosphoenolpyruvate carboxykinase (PEPCK) were both up-regulated after treatment with trilobatin. Expression of GLUT2, PEPCK and G-6-P were also increased in liver tissue after treatment with phloridzin. Our data indicate that leaf extract from L. polystachyus Rehd. has a preferable hypoglycemic effects than trilobatin or phloridzin alone. Leaf extract significantly increased glucose uptake and hepatic glycogen synthesis while also inducing a decline of hepatic gluconeogenesis and oxidative stress in T2DM mice. From this study, we draw conclusions that L. polystachyus promoted glycogen synthesis in T2DM mice, and that the active compounds were not only the trilobatin or phloridzin.

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Enrichment and fractionation of major apple flavonoids, phenolic acids and dihydrochalcones using anion exchange resins

Cited by 44 publications

References 17 publications

Recovery and fractionation of major apple and grape polyphenols from model solutions and crude plant extracts using ion exchange and adsorbent resins

Recovery and fractionation of major apple and grape polyphenols from model solutions and crude plant extracts using ion exchange and adsorbent resins

Recovery and Concentration of Antioxidants from Winery Wastes

Leaf Extract from Lithocarpus polystachyus Rehd. Promote Glycogen Synthesis in T2DM Mice

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