Enrichment and Characterization of Human Dermal Stem/Progenitor Cells by Intracellular Granularity

Shim, Ji Sung; Lee, Tae Ryong; Shin, Dong Wook

doi:10.1089/scd.2012.0244

Cited by 10 publications

(9 citation statements)

References 61 publications

(117 reference statements)

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“…It is also difficult to obtain adult stem cells because of their limited quantities in the tissue. According to some previous literatures, they are considered to be composed of 0.01% to 0.001% of the total cell number (Hill et al, 2012;Rendl et al, 2005;Riekstina et al, 2009;Shim et al, 2013;Wegner and Stolt, 2005;Wu et al, 2010). We found that hDSPCs in small quantity gradually lose pluripotency and the ability to proliferate once they are grown over long-term culture.…”

Section: Introductionmentioning

confidence: 80%

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O 2 concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.

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Section: Introductionmentioning

confidence: 80%

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Shim

Lee

Shin

2013

Molecules and Cells

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“…Unexpectedly, corin expression was detected in the dermal papilla of hair follicles in mice and humans [54, 55]. The biological significance of corin in the skin is not completely understood.…”

Section: Mouse Models Of Corin Deficiencymentioning

confidence: 99%

Corin in Natriuretic Peptide Processing and Hypertension

Zhou

2014

Curr Hypertens Rep

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Corin is a serine protease originally isolated from the heart. Functional studies show that corin is the long-sought enzyme responsible for activating cardiac natriuretic peptides. In mice, lack of corin prevents the natriuretic peptide processing, causing salt-sensitive hypertension. In humans, corin variants and mutations that reduce corin activity have been identified in patients with hypertension and heart failure. Decreased plasma levels of corin antigen and activity have been reported in patients with heart failure and coronary artery disease. Low levels of urinary corin also have been found in patients with chronic kidney disease. Most recent studies show that corin also acts in the uterus to promote spiral artery remodeling and prevent pregnancy-induced hypertension. Here we review the role of corin in natriuretic peptide processing and cardiovascular diseases such as hypertension, heart disease, pre-eclampsia, and chronic kidney disease.

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“…The isolation of skinderived precursor cells with neurogenic properties has been previously reported from human dermis [13][14][15] and hair follicles [16] as have the isolation and partial purification of adult multipotent stem cells from human and murine epidermis [17,18]. For instance, Fujimori et al reported the isolation of human epidermal stem/progenitor cells by means of gravity-assisted cell sorting [17].…”

mentioning

confidence: 99%

Equine Epidermis: A Source of Epithelial-Like Stem/Progenitor Cells with In Vitro and In Vivo Regenerative Capacities

Broeckx¹,

Maes²,

Martinello

et al. 2014

Stem Cells and Development

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Besides the presence of somatic stem cells in hair follicles and dermis, the epidermis also contains a subpopulation of stem cells, reflecting its high regenerative capacity. However, only limited information concerning epidermis-derived epithelial-like stem/progenitor cells (EpSCs) is available to date. Nonetheless, this stem cell type could prove itself useful in skin reconstitution after injury. After harvesting from equine epidermis, the purified cells were characterized as EpSCs by means of positive expression for CD29, CD44, CD49f, CD90, Casein Kinase 2b, p63, and Ki67, low expression for cytokeratin (CK)14 and negative expression for CD105, CK18, Wide CK, and Pan CK. Furthermore, their self-renewal capacity was assessed in adhesion as well as in suspension. Moreover, the isolated cells were differentiated toward keratinocytes and adipocytes. To assess the regenerative capacities of EpSCs, six full-thickness skin wounds were made: three were treated with EpSCs and platelet-rich-plasma (EpSC/PRP-treated), while the remaining three were administered carrier fluid alone (PRP-treated). The dermis of EpSC/PRP-treated wounds was significantly thinner and exhibited more restricted granulation tissue than did the PRP-treated wounds. The EpSC/PRP-treated wounds also exhibited increases in EpSCs, vascularization, elastin content, and follicle-like structures. In addition, combining EpSCs with a PRP treatment enhanced tissue repair after clinical application.

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Enrichment and Characterization of Human Dermal Stem/Progenitor Cells by Intracellular Granularity

Cited by 10 publications

References 61 publications

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Novel In Vitro Culture Condition Improves the Stemness of Human Dermal Stem/Progenitor Cells

Corin in Natriuretic Peptide Processing and Hypertension

Equine Epidermis: A Source of Epithelial-Like Stem/Progenitor Cells with In Vitro and In Vivo Regenerative Capacities

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