Enoyl-Coenzyme A Hydratase and Antigen 85B of Mycobacterium habana Are Specifically Recognized by Antibodies in Sera from Leprosy Patients

Serafín‐López, Jeanet; Talavera-Paulin, Moises; Amador-Molina, Juan C.; Alvarado-Riverón, M; Vilchis-Landeros, M. Magdalena; Méndez-Ortega, P; Fafutis‐Morris, Mary; Paredes‐Cervantes, Vladimir; López‐Santiago, Rubén; León, C. Inés; Guerrero, Miguel; Ribas‐Aparicio, Rosa María; Mendoza‐Hernández, Guillermo; Carreño-Martinez, Cristina; Estrada‐Parra, Sergio; Estrada-Garcı́a, Iris

doi:10.1128/cvi.00519-10

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…All of these changes can be explained as the adsorption of negatively charged Ag85B (pI = 5.5) onto SWCNTs, inducing positive charges, p-doping SWCNTs, and increasing the mobility. 49 − 52 The calibration curve for the detection of Ag85B proteins spiked in PBS is depicted in Figure 3 b. The calibration curve was linearly fit ( y = 0.4066 × log( x ) + 6.6724), and a linear correlation coefficient of 0.9804 was obtained ( Figure S10 ), showing a large dynamic range of this Ab85B-SWCNT device.…”

Section: Resultsmentioning

confidence: 98%

“…With the increasing antigen concentration, the characteristic curves shifted to more positive gate voltages, the threshold voltage showed an increasing trend (Figure S8), and the curve’s linear region slope decreased (Figure S9). All of these changes can be explained as the adsorption of negatively charged Ag85B (pI = 5.5) onto SWCNTs, inducing positive charges, p-doping SWCNTs, and increasing the mobility. − The calibration curve for the detection of Ag85B proteins spiked in PBS is depicted in Figure b. The calibration curve was linearly fit ( y = 0.4066 × log( x ) + 6.6724), and a linear correlation coefficient of 0.9804 was obtained (Figure S10), showing a large dynamic range of this Ab85B-SWCNT device.…”

Section: Resultsmentioning

confidence: 99%

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Diagnostics of Tuberculosis with Single-Walled Carbon Nanotube-Based Field-Effect Transistors

Wang,

Shao,

Liu

et al. 2024

ACS Sens.

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Tuberculosis (TB) is still threatening millions of people's lives, especially in developing countries. One of the major factors contributing to the ongoing epidemic of TB is the lack of a fast, efficient, and inexpensive diagnostic strategy. In this work, we developed a semiconducting single-walled carbon nanotube (SWCNT)-based field-effect transistor (FET) device functionalized with anti-Mycobacterium tuberculosis antigen 85B antibody (Ab85B) to detect the major M. tuberculosis-secreted antigen 85B (Ag85B). Through optimizing the device fabrication process by evaluating the mass of the antibody and the concentration of the gating electrolyte, our Ab85B-SWCNT FET devices achieved the detection of the Ag85B spiked in phosphate-buffered saline (calibration samples) with a limit of detection (LOD) of 0.05 fg/ mL. This SWCNT FET biosensor also showed good sensing performance in biological matrices including artificial sputum and can identify Ag85B in serum after introducing bovine serum albumin (BSA) into the blocking layer. Furthermore, our BSA-blocked Ab85B-SWCNT FET devices can distinguish between TB-positive and -negative clinical samples, promising the application of SWCNT FET devices in point-of-care TB diagnostics. Moreover, the robustness of this SWCNT-based biosensor to the TB diagnosis in blood serum was enhanced by blocking SWCNT devices directly with a glutaraldehyde cross-linked BSA layer, enabling future applications of these SWCNT-based biosensors in clinical testing.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Diagnostics of Tuberculosis with Single-Walled Carbon Nanotube-Based Field-Effect Transistors

Wang,

Shao,

Liu

et al. 2024

ACS Sens.

View full text Add to dashboard Cite

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“…Previously, it has been postulated that mycoloyltransferases may be post-translationally modified, ,,− which could possibly explain their ability to associate with the cell envelope. However, to date, these enzymes have not been demonstrated to be modified, and their mechanism of cell envelope association remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Chemical Proteomics Strategies for Analyzing Protein Lipidation Reveal the Bacterial O-Mycoloylome

Banahene,

Peters-Clarke,

Biegas

et al. 2024

J. Am. Chem. Soc.

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Protein lipidation dynamically controls protein localization and function within cellular membranes. A unique form of protein O-fatty acylation in Corynebacterium, termed protein O-mycoloylation, involves the attachment of mycolic acids�unusually large and hydrophobic fatty acids�to serine residues of proteins in these organisms' outer mycomembrane. However, as with other forms of protein lipidation, the scope and functional consequences of protein Omycoloylation are challenging to investigate due to the inherent difficulties of enriching and analyzing lipidated peptides. To facilitate the analysis of protein lipidation and enable the comprehensive profiling and site mapping of protein Omycoloylation, we developed a chemical proteomics strategy integrating metabolic labeling, click chemistry, cleavable linkers, and a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method employing LC separation and complementary fragmentation methods tailored to the analysis of lipophilic, MS-labile O-acylated peptides. Using these tools in the model organism Corynebacterium glutamicum, we identified approximately 30 candidate Omycoloylated proteins, including porins, mycoloyltransferases, secreted hydrolases, and other proteins with cell envelope-related functions�consistent with a role for O-mycoloylation in targeting proteins to the mycomembrane. Site mapping revealed that many of the proteins contained multiple spatially proximal modification sites, which occurred predominantly at serine residues surrounded by conformationally flexible peptide motifs. Overall, this study (i) discloses the putative protein O-mycoloylome for the first time, (ii) yields new insights into the undercharacterized proteome of the mycomembrane, which is a hallmark of important pathogens (e.g., Corynebacterium diphtheriae, Mycobacterium tuberculosis), and (iii) provides generally applicable chemical strategies for the proteomic analysis of protein lipidation.

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Leprosy Vaccines: Developments for Prevention and Treatment

Wang

2023

Vaccines for Neglected Pathogens: Strategies, Achievements and Challenges

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Over 200,000 new leprosy cases are reported globally every year. A vaccine for leprosy can eliminate the debilitating, biblical, and stigmatised disease in the twenty-first century. Since the 1940s, many clinical studies have consistently shown that the BCG vaccine offers some level of protection but ranging between 18% and 90%. Throughout this time, different versions of BCG and new developments have resulted in new leprosy vaccine candidates and prevention strategies. Examples are the vaccine and drug combinatory therapy that has shown promise in decreasing transmission and the subunit vaccine candidate, LepVax, which has been shown to reduce bacterial count and delay nerve function impairment in animal models and safe in healthy adults in early studies. The WHO officially recommended the BCG vaccine as a leprosy vaccine in 2018, a century later after it was first used as a tuberculosis vaccine in 1921. However, a better leprosy vaccine and prevention strategy is still needed because we do not exactly know how Mycobacterium leprae spreads and causes neurological damage in leprosy patients. The history and latest developments in leprosy vaccines are explored in this chapter.

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Enoyl-Coenzyme A Hydratase and Antigen 85B of Mycobacterium habana Are Specifically Recognized by Antibodies in Sera from Leprosy Patients

Cited by 5 publications

References 30 publications

Diagnostics of Tuberculosis with Single-Walled Carbon Nanotube-Based Field-Effect Transistors

Diagnostics of Tuberculosis with Single-Walled Carbon Nanotube-Based Field-Effect Transistors

Chemical Proteomics Strategies for Analyzing Protein Lipidation Reveal the Bacterial O-Mycoloylome

Leprosy Vaccines: Developments for Prevention and Treatment

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