Enorma shizhengliae sp. nov. and Eggerthella guodeyinii sp. nov., two new members of the family Coriobacteriaceae

Abstract

Four obligatory anaerobic, Gram-stain-positive, non-motile and rod-shaped organisms (HF-1365T, HF-1362, HF-1101T and HF-4214) were isolated from faecal samples of healthy Chinese subjects. Results of 16S rRNA gene sequence analyses showed that these isolates belong to the genera Enorma (strains HF-1365T and HF-1362) and Eggerthella (strains HF-1101T and HF-4214), closest to Enorma massiliensis (both 98.6 %) and Eggerthella sinensis (98.0 and 97.8 %), respectively. The whole genome sequences of strains HF-1365T and HF-1101T were 2.3 and 4.2 Mb in size with 61.7 and 66.2 mol% DNA G+C content, respectively. The average nucleotide identity and digital DNA–DNA hybridization values indicated that strains HF-1365T and HF-1101T represent novel species in the genera Enorma and Eggerthella . Major fatty acid constituents (>10 %) of strains HF-1365T and HF-1362 were C12 : 0 (24.7 and 23.9 %), C14 : 0 (21.9 and 20.6 %) and summed feature 1 (C15 : 1iso H/C13 : 0 3OH; 12.8 and 10.8 %); those of strains HF-1101T and HF-4214 were C18 : 1 ω9c (32.4 and 33.1 %) and C16 : 0 (13.9 and 14.0 %). Strain HF-1365T had phospholipid, glycolipid, lipid and phosphoglycolipid without any known quinones, while strain HF-1101T had diphosphatidylglycerol as the major polar lipid and MK-7 (80.7 %) as the predominant quinone. On the basis of their phylogenetic and phenotypic characteristics, strains HF-1365T and HF-1101T represent two distinct species, respectively, in the genera Enorma and Eggerthella , for which the names Enorma shizhengliae sp. nov. (type strain HF-1365T=CGMCC 1.17435T=GDMCC 1.1705T=JCM 33601T) and Eggerthella guodeyinii sp. nov. (type strain HF-1101T=CGMCC 1.17436T=GDMCC 1.1668T=JCM 33773T) are proposed.

New members of the family Eggerthellaceae isolated from Marmota himalayana: Xiamenia xianingshaonis gen. nov., sp. nov., from intestinal contents, and Berryella wangjianweii sp. nov., from trachea

“…In contrast to the absence of any known quinones in zg-1050 T , strain zg-886 T contained MK-6 (42.5%), DMMK-6 (32.5%) and MMK-6 (25%), which is distinct from other type species in in its menaquinone types and the corresponding relative percentages [7, 8, 34, 36, 42, 43], except those six with no available data ( Berryella intestinalis 68-1-3 T , Denitrobacterium detoxificans NPOH1 T , Hugonella massiliensis AT8 T , Phoenicibacter congonensis Marseille-P3241 T , Raoultibacter massiliensis Marseille-P2849 T and Slackia exigua ATCC 700122 T ).…”

“…Although C 16 : 0 and C 18 : 1 ω 9 c were the two most abundant CFAs in all novel strains, C 12 : 0 reached a dominant level in zg-1050 T /zg-1084, whereas three different CFAs (C 14 : 0 , iso - C 14 : 0 and C 18 : 0 ) were present at high levels (>10 %) in zg-886 T /zg-887 ( and S4). Moreover, the predominant level of C 18 : 0 in zg-886 T /zg-887 and Berryella intestinalis 68-1-3 T () [6] was in sharp contrast to the modest amount in others (zg-1050 T /zg-1084, Table S4 and Raoultibacter massiliensis Marseille-P2849 T ) [6–9, 20, 33, 34, 36, 42–44].…”