Enolase is present in the cell wall of<i>Saccharomyces cerevisiae</i>

Edwards, Scott A.; Braley, R; Chaffin, W. LaJean

doi:10.1111/j.1574-6968.1999.tb13734.x

Cited by 63 publications

(8 citation statements)

References 28 publications

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“…In our study, 90.31% of the xylem sap proteins were secreted proteins, which was composed of 56.98% classical peptides and 33.33% non-classical peptides; 9.69% were non-secreted proteins, e.g., lactoylglutathione lyase (glyoxalase I) (an enolase). Similarly, enolase has been secreted to the cell wall or extracellular space via immunolocalization even though it lacked a signal peptide 30 . Furthermore, glyoxalase 1 has been identified in the cell wall proteome of the maize primary root elongation zone 31 and in a cell wall proteomics study of mature alfalfa stems 32 .…”

Section: Discussionmentioning

confidence: 99%

Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses

Zhang

Chao

Wang

et al. 2016

Sci Rep

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Proteomics was employed to investigate the molecular mechanisms of apoplastic response to potassium(K)-deficiency in cotton. Low K (LK) treatment significantly decreased the K and protein contents of xylem sap. Totally, 258 peptides were qualitatively identified in the xylem sap of cotton seedlings, of which, 90.31% were secreted proteins. Compared to the normal K (NK), LK significantly decreased the expression of most environmental-stress-related proteins and resulted in a lack of protein isoforms in the characterized proteins. For example, the contents of 21 Class Ш peroxidase isoforms under the LK were 6 to 44% of those under the NK and 11 its isoforms were lacking under the LK treatment; the contents of 3 chitinase isoforms under LK were 11–27% of those under the NK and 2 its isoforms were absent under LK. In addition, stress signaling and recognizing proteins were significantly down-regulated or disappeared under the LK. In contrast, the LK resulted in at least 2-fold increases of only one peroxidase, one protease inhibitor, one non-specific lipid-transfer protein and histone H4 and in the appearance of H2A. Therefore, K deficiency decreased plant tolerance to environmental stresses, probably due to the significant and pronounced decrease or disappearance of a myriad of stress-related proteins.

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Section: Discussionmentioning

confidence: 99%

Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses

Zhang

Chao

Wang

et al. 2016

Sci Rep

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“…In addition, it is important to note that a number of proteins identified in this study are homologous to known non-classical secreted proteins in other organisms, including bacteria, yeast, and mammals for which experimental evidence exists. Examples are annexin, enolase and elongation factor ( Edwards et al, 1999 ; Marcilla et al, 2012 ; Marques et al, 1998 ). One such protein, elongation factor 1α that lacks a signal peptide was isolated from tobacco and has been shown to localize to the cell wall by immunogold localization ( Zhu et al, 1994 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis to Identify Tightly-Bound Cell Wall Protein in Rice Calli

Cho¹,

Hyun²,

Kumar³

et al. 2015

Molecules and Cells

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Rice is a model plant widely used for basic and applied research programs. Plant cell wall proteins play key roles in a broad range of biological processes. However, presently, knowledge on the rice cell wall proteome is rudimentary in nature. In the present study, the tightly-bound cell wall proteome of rice callus cultured cells using sequential extraction protocols was developed using mass spectrometry and bioinformatics methods, leading to the identification of 1568 candidate proteins. Based on bioinformatics analyses, 389 classical rice cell wall proteins, possessing a signal peptide, and 334 putative non-classical cell wall proteins, lacking a signal peptide, were identified. By combining previously established rice cell wall protein databases with current data for the classical rice cell wall proteins, a comprehensive rice cell wall proteome, comprised of 496 proteins, was constructed. A comparative analysis of the rice and Arabidopsis cell wall proteomes revealed a high level of homology, suggesting a predominant conservation between monocot and eudicot cell wall proteins. This study importantly increased information on cell wall proteins, which serves for future functional analyses of these identified rice cell wall proteins.

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“…(61) Studies with other microbes, including fungi, have found metabolic proteins outside of the cytosol, including phosphoglycerate kinase (Pgk1) and enolase (Eno1). 59,62−64 In addition to a role in glycolysis, enolases from Pneumocystis and Leishmania have been shown to bind host plasminogen. 65,66 …”

Section: Discussionmentioning

confidence: 99%

Definition of the Extracellular Proteome of Pathogenic-PhaseHistoplasma capsulatum

Holbrook¹,

Edwards²,

Youseff³

et al. 2011

J. Proteome Res.

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The dimorphic fungal pathogen Histoplasma capsulatum causes respiratory and systemic disease. Within the mammalian host, pathogenic Histoplasma yeast infect, replicate within, and ultimately kill host phagocytes. Surprisingly, few factors have been identified that contribute to Histoplasma virulence. To address this deficiency, we have defined the constituents of the extracellular proteome using LC−MS/MS analysis of the proteins in pathogenic-phase culture filtrates of Histoplasma. In addition to secreted Cbp1, the extracellular proteome of pathogenic Histoplasma yeast consists of 33 deduced proteins. The proteins include glycanases, extracellular enzymes related to oxidative stress defense, dehydrogenase enzymes, chaperone-like factors, and five novel culture filtrate proteins (Cfp’s). For independent verification of proteomics-derived identities, we employed RNA interference (RNAi)-based depletion of candidate factors and showed loss of specific proteins from the cell-free culture filtrate. Quantitative RT-PCR revealed the expression of 10 of the extracellular factors was particularly enriched in pathogenic yeast cells as compared to nonpathogenic Histoplasma mycelia, suggesting that these proteins are linked to Histoplasma pathogenesis. In addition, Histoplasma yeast express these factors within macrophages and during infection of murine lungs. As extracellular proteins are positioned at the interface between host and pathogen, the definition of the pathogenic-phase extracellular proteome provides a foundation for the molecular dissection of how Histoplasma alters the host-pathogen interaction to its advantage.

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Enolase is present in the cell wall ofSaccharomyces cerevisiae

Cited by 63 publications

References 28 publications

Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses

Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses

Proteomic Analysis to Identify Tightly-Bound Cell Wall Protein in Rice Calli

Definition of the Extracellular Proteome of Pathogenic-PhaseHistoplasma capsulatum

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