ENO1 gene product binds to the c‐<i>myc</i> promoter and acts as a transcriptional repressor: relationship with Myc promoter‐binding protein 1 (MBP‐1)

Feo, Salvatore; Arcuri, Daniela; Piddini, Eugenia; Passantino, Rosa; Giallongo, Agata

doi:10.1016/s0014-5793(00)01494-0

Cited by 256 publications

(237 citation statements)

References 44 publications

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“…Enolase 1 (P06733, P17182) (also known as alpha-enolase or Plasminogen-binding protein) is a highly abundant 48 kDa cytosolic enzyme which catalyzes the reversible dehydration of 2-phospho-D-glycerate to phosphoenolpyruvate as a part of the glycolytic and gluconeogenesis pathways. Aside from its primary cytosolic role, enolase 1 can also be found on the cell surface serving as a plasminogen receptor [4] and in the nucleus, as an alternatively translated 37 kDa tumor suppressor c-myc promoter-binding protein (MBP-1) [5,6]. Tau-crystallin, the principal component of reptile and some avian eye lens is also coded by eno1 gene [7].…”

Section: Is Enolase 1 a Universal Sensor Regulator Or A Stress Chapmentioning

confidence: 99%

“…We compiled the identities of differentially expressed proteins identified in human, mouse, and rat tissues by 2-DE-based experiments from three recent volumes of Proteomics (volumes [4][5][6][2004][2005][2006] and calculated the appearance of each protein in the dataset. Added to the dataset were all experiments performed with total cellular homogenates.…”

Section: Introductionmentioning

confidence: 99%

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Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins

et al. 2008

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After reading many 2-DE-based articles featuring lists of the differentially expressed proteins, one starts experiencing a disturbing déjà vu. The same proteins seem to predominate regardless of the experiment, tissue or species. To quantify the occurrence of individual differentially expressed proteins in 2-DE experiment reports, we compiled the identities of differentially expressed proteins identified in human, mouse, and rat tissues published in three recent volumes of Proteomics and calculated the appearance of the most predominant proteins in the dataset. The most frequently identified protein is a highly abundant glycolytic enzyme enolase 1, differentially expressed in nearly every third experiment on both human and rodent tissues. Heat-shock protein 27 (HSP27) and heat-shock protein 60 (HSP60) were differentially expressed in about 30 percent of human and rodent samples, respectively. Considering protein families as units, keratins and peroxiredoxins are the most frequently identified molecules, with at least one member of the group being differentially expressed in about 40 percent of all experiments. We suggest that the frequent identification of these proteins must be considered in the interpretation of any 2-DE studies. We consider if these commonly observed changes represent common cellular stress responses or are a reflection of the technical limitations of 2-DE.

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Section: Is Enolase 1 a Universal Sensor Regulator Or A Stress Chapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins

et al. 2008

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“…NPM/ B23 and enolase-α), which play such different functional roles in the cell, interrelate in the promitogenic/prosurvival bystander response? It has been reported that enolase-α also plays an important role in the regulation of c-myc promoter activity in the form of alternative translation product MBP-1 (37-40 kDa), which is distinct from its role as a glycolytic enzyme [59][60][61][62]. The c-myc proto-oncogene is known as an essential part of normal cell proliferative machinery [63,64].…”

Section: Cooperation Of Npm and Enolase-α In Pbrmentioning

confidence: 99%

“…The c-myc proto-oncogene is known as an essential part of normal cell proliferative machinery [63,64]. MPB-1, structurally very similar to enolase-α, binds to the c-myc P2 promoter and down-regulates c-myc expression, and therefore, suppresses cell proliferation [60][61][62]. Unlike enolase-α, which is mainly localized in the cytoplasm, MPB-1 has been primarily found in the cell nuclei [61].…”

Section: Cooperation Of Npm and Enolase-α In Pbrmentioning

confidence: 99%

“…MPB-1, structurally very similar to enolase-α, binds to the c-myc P2 promoter and down-regulates c-myc expression, and therefore, suppresses cell proliferation [60][61][62]. Unlike enolase-α, which is mainly localized in the cytoplasm, MPB-1 has been primarily found in the cell nuclei [61]. Since under stress conditions, enolase-α is an easy target for protein modifications, there is a good probability that MPB-1 could be readily modified as well which could inhibit down-regulation of c-myc expression.…”

Section: Cooperation Of Npm and Enolase-α In Pbrmentioning

confidence: 99%

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Proteome analysis of proliferative response of bystander cells adjacent to cells exposed to ionizing radiation

Gerashchenko

Yamagata²,

Oofusa³

et al. 2007

Proteomics

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Recently (Cytometry 2003, 56A, 71-80), we reported that direct cell-to-cell contact is required for stimulating proliferation of bystander rat liver cells (WB-F344) cocultured with irradiated cells, and neither functional gap junction intercellular communication nor long-range extracellular factors appear to be involved in this proliferative bystander response (PBR). The molecular basis for this response is unknown. Confluent monolayers of WB-F344 cells were exposed to 5-Gray (Gy) of γ-rays. Irradiated cells were mixed with unirradiated cells and co-cultured for 24 h. Cells were harvested and protein expression was examined using 2-DE. Protein expression was also determined in cultures of unirradiated and 5-Gy irradiated cells. Proteins were identified by MS. Nucleophosmin (NPM)-1, a multifunctional nucleolar protein, was more highly expressed in bystander cells than in either unirradiated or 5-Gy irradiated cells. Enolase-α, a glycolytic enzyme, was present in acidic and basic variants in unirradiated cells. In bystander and 5-Gy irradiated cells, the basic variant was weakly expressed, whereas the acidic variant was overwhelmingly present. These data indicate that the presence of irradiated cells can affect NPM-1 and enolase-α in adjacent bystander cells. These proteins appear to participate in molecular events related to the PBR and suggest that this response may involve cellular defense, proliferation, and metabolism.

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Gene expression patterns in the bone tissue of women with fibrous dysplasia

Kiss

Balla

Kósa

et al. 2010

American J of Med Genetics Pt A

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Fibrous dysplasia is an isolated skeletal disorder caused by a somatic activating mutation of GNAS gene with abnormal unmineralized matrix overproduction and extensive undifferentiated bone cell accumulation in the fibro-osseous lesions. The aim of our investigation was to identify genes that are differently expressed in fibrous versus non-fibrous human bone and to describe the relationships between these genes using multivariate data analysis. Six bone tissue samples from female patients with fibrous dysplastia (FD) and seven bone tissue samples from women without FD (non-FD) were examined. The expression differences of selected 118 genes were analyzed by the TaqMan probe-based quantitative real-time RT-PCR system. The Mann-Whitney U-test indicated marked differences in the expression of 22 genes between FD and non-FD individuals. Nine genes were upregulated in FD women compared to non-FD ones and 18 genes showed a downregulated pattern. These altered genes code for minor collagen molecules, extracellular matrix digesting enzymes, transcription factors, adhesion molecules, growth factors, pro-inflammatory cytokines, and lipid metabolism-affected substrates. Canonical variates analysis demonstrated that FD and non-FD bone tissues can be distinguished by the multiple expression profile analysis of numerous genes controlled via a G-protein coupled pathway and BMP cascade as well as genes coding for extracellular matrix composing molecules. The remarkable changed gene expression profile observed in the fibrous dysplastic human bone tissue may provide further insight into the pathogenetic process of fibrous degeneration of bone.

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ENO1 gene product binds to the c‐myc promoter and acts as a transcriptional repressor: relationship with Myc promoter‐binding protein 1 (MBP‐1)

Abstract: The Myc promoter-binding protein-1 (MBP-

Cited by 256 publications

References 44 publications

Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins

Déjà vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins

Proteome analysis of proliferative response of bystander cells adjacent to cells exposed to ionizing radiation

Gene expression patterns in the bone tissue of women with fibrous dysplasia

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