ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment

Sun, Hongxia; Mo, Jing; Cheng, Run-Fen; Li, Fan; Li, Yue; Guo, Yuhong; Li, Yanlei; Zhang, Yanhui; Bai, Xiaoyu; Wang, Yalei; Dong, Xueyi; Zhang, Danfang; Hao, Jihui

doi:10.20892/j.issn.2095-3941.2022.0451

Cited by 3 publications

(2 citation statements)

References 41 publications

(50 reference statements)

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“…Weekly passaging at 1:4 to 1:8 split ratios was achieved by mechanical disruption using flame‐polished Pasteur pipettes. For experiments where it is indicated, small‐molecule inhibitor Senkyunolide I (SENI) was used in a concentration of 5 μM as described previously 27,28 …”

Section: Methodsmentioning

confidence: 99%

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Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death

Csukovich,

Wagner,

Walter

et al. 2023

Cell Proliferation

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Apical‐out intestinal organoids are a relatively simple method of gaining access to the apical cell surface and have faced increasing scientific interest over the last few years. Apical‐out organoids can thus be used for disease modelling to compare differing effects on the basolateral versus the apical cell surface. However, these ‘inside‐out’ organoids die relatively quickly and cannot be propagated as long as their basal‐out counterparts. Here, we show that apical‐out organoids have drastically reduced proliferative potential, as evidenced by immunohistochemical staining and the incorporation of the thymidine analogue EdU. At the same time, cell death levels are increased. Nevertheless, these phenomena cannot be explained by an induction of differentiation, as the gene expression of key marker genes for various cell types does not change over time.

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Section: Methodsmentioning

confidence: 99%

“…For experiments where it is indicated, small‐molecule inhibitor Senkyunolide I (SENI) was used in a concentration of 5 μM as described previously. 27 , 28 …”

Section: Methodsmentioning

confidence: 99%

Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death

Csukovich,

Wagner,

Walter

et al. 2023

Cell Proliferation

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Mechanism of the apoptosis of bone marrow erythroblasts in rats under hypobaric hypoxia

Ma,

Wang,

Liu

et al. 2024

Blood Cells, Molecules, and Diseases

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Molecular bioinformatic and functional analysis of Enolase4 gene during siphonal autotomy and regeneration of razor clam Solen grandis

Zhang,

Sun,

Chen

et al. 2023

Front. Mar. Sci.

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For the razor clam Solen grandis, siphonal autotomy and regeneration is a necessary means of facing external stress, which is accompanied by a large amount of energy consumption. To explore the function of the ENO4 gene in the siphonal autotomy and regeneration of S. grandis, the full-length cDNA of the ENO4 in S. grandis was cloned and characterized. The full-length cDNA of SgENO4 was 2,773 base pairs (bp), with an 1,851 bp coding sequence (CDS) encoding 616 amino acids. Bioinformatic analysis revealed a slightly lower codon usage bias and a relatively stable nucleotide composition in the SgENO4 sequence. Three conserved motifs were found from the SgENO4 sequence. These conserved motifs vary in length from 14 to 27 amino acids. Moreover, in the siphonal pre-autotomy of razor clams, expression level of the SgENO4 gene was significantly higher in the hepatopancreas and siphonal base than other tissues (P < 0.05). However, the expression of the SgENO4 gene in the siphonal base decreased significantly in the siphonal post-autotomy 7 hours and gradually recovered as the regeneration process continued. Linear correlation analysis showed that there was a significant linear correlation between specific weight of siphon and relative expression levels of SgENO4 (R2 = 0.826). RNA interference (RNAi) result showed that the specific weight (the proportion of siphonal weight to body weight) is significantly lower than other groups on the seventh day (P < 0.05), suggesting that interfering with the expression of the SgENO4 gene could inhibit the siphonal regeneration of S. grandis. These results further demonstrate that SgENO4 is a conserved sequence and that it plays a crucial role in the siphonal autotomy and regeneration of S. grandis, advancing further understanding in exploring molecular mechanisms in the siphonal autotomy and regeneration.

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ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment

Cited by 3 publications

References 41 publications

Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death

Polarity reversal of canine intestinal organoids reduces proliferation and increases cell death

Mechanism of the apoptosis of bone marrow erythroblasts in rats under hypobaric hypoxia

Molecular bioinformatic and functional analysis of Enolase4 gene during siphonal autotomy and regeneration of razor clam Solen grandis

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