ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

Gu, Jinrong; Zhong, Kaiqiang; Wang, Longgang; Ni, Haishun; Zhao, Yongxiang; Wang, Xuchao; Yao, Yizhou; Jiang, Linhua; Wang, Bin; Zhu, Xinguo

doi:10.3389/fonc.2022.1013035

Cited by 6 publications

(2 citation statements)

References 46 publications

(52 reference statements)

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“…Interestingly, by single-cell sequencing data analysis of CD8 + T cells, we found that ENO1 and inhibitory receptors such as LAG3 were upregulated with the progression of CD8 + T-cell exhaustion in our study, which was consistent with the results of L.F. Gemta's study and suggested that ENO1 may undergo posttranslational modi cation in exhausted CD8 + T cells [17,47]. ENO1 can, through the regulation of the cell cycle[48] and apoptosis, maintain cancer cell proliferation and resistance to cell death [49], and through the induction of EMT, promote invasion and metastasis [50].…”

Section: Discussionsupporting

confidence: 91%

Multiomics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy

Su,

You,

et al. 2023

Preprint

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Background α-Enolase (ENO1) is a crucial molecular target for tumor therapy and has emerged as a research hotspot in recent decades. Here, we aimed to explore the role of ENO1 in bladder cancer (BLCA) and then construct a signature to predict the prognosis and treatment response of BLCA.Methods Differential expression, prognosis analysis and in vitro cell experiments were used to reveal the value of ENO1 in BLCA. The R package "Seurat" was used for single-cell RNA sequence (scRNA-seq) data processing. The R package “singleR” and cellMarker website were used to annotate cells. The FindAllMarkers function and “limma” were used to screen hub genes. Univariate Cox and least absolute shrinkage and selection operator (LASSO) analyses were used to construct the signature. Differences in prognosis and treatment between high- and low-risk groups were investigated.Results ENO1 was highly expressed in BLCA tissues, as verified by IHC, and was associated with poor prognosis. The analysis of the tumor immune microenvironment by bulk sequencing and scRNA-seq showed that ENO1 was associated with CD8 + T-cell exhaustion. Additionally, the in vitro results showed that ENO1 could promote the proliferation and invasion of BLCA cells. Then, the analysis of epithelial cells (ECs) revealed that ENO1 might promote BLCA progression by metabolism, the cell cycle and some carcinogenic pathways. A total of 249 hub genes were obtained from differentially expressed genes between ENO1-related ECs, and we used LASSO analysis to construct a novel signature that not only accurately predicted the prognosis of BLCA patients but also predicted the response to treatment for BLCA. Finally, we constructed a nomogram to better guide clinical application.Conclusion Through multiomics analysis, we found that ENO1 was overexpressed in bladder cancer and associated with poor prognosis, CD8 + T-cell exhaustion and epithelial heterogeneity. Finally, the prognosis and treatment of patients can be well predicted by constructing an epithelial cell prognostic signature.

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Section: Discussionsupporting

confidence: 91%

Multiomics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy

Su,

You,

et al. 2023

Preprint

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“…In addition to its role as a glycolytic enzyme, α-enolase (ENO1), the most extensively studied isoform of ENO, is expressed on the cell surface of most tumors and has been shown to be an oncogenic factor in multiple cancers that depends on the modulation of a variety of biological events, such as glycolysis, angiogenesis, invasion and metastasis (10,11). In particular, interference with ENO1 has been shown to decrease glycolysis, cell growth, metastasis and chemotherapy resistance in CRC (12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

ENO1 deletion potentiates ferroptosis and decreases glycolysis in colorectal cancer cells via AKT/STAT3 signaling

Liu,

Hou,

Zhang

et al. 2024

Exp Ther Med

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Colorectal cancer (CRC) is one of the most prevailing and lethal forms of cancer globally. α-enolase (ENO1) has been well documented to be involved in the progression and drug resistance of CRC. The present study was designed to specify the role of ENO1 in major events during the process of CRC and to introduce its latent functional mechanism. ENO1 expression was determined by western blot analysis. Extracellular acidification rates were assessed using an XF96 extracellular flux analyzer. Glucose uptake, lactic acid production, total iron levels and ferroptosis-related markers were examined with corresponding kits. A dichlorodihydrofluorescein diacetate probe measured intracellular reactive oxygen species content. Western blotting detected the expression of glycolysis- and ferroptosis-related proteins. CCK-8 and EdU staining assays assessed cell proliferation. In the current study, ENO1 was highly expressed in CRC cells. Knockdown of ENO1 markedly reduced the glycolysis and accelerated the ferroptosis in CRC cells. Moreover, the inhibitory effects of WZB117, a specific inhibitor of glycolysis-related glucose transporter type 1, on CRC cell proliferation were further enhanced by ENO1 interference. In addition, silencing of ENO1 inactivated the AKT/STAT3 signaling. The AKT activator SC79 partially reversed the effects of ENO1 deficiency on the AKT/STAT3 signaling, glycolysis, proliferation as well as ferroptosis in CRC cells. In summary, inactivation of AKT/STAT3 signaling mediated by ENO1 inhibition might boost the ferroptosis and suppress the glycolysis in CRC cells.

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Exploring the role of estrogen and progestins in breast cancer: A genomic approach to diagnosis

Selvam,

Elavarasu,

Dhanushkumar

et al. 2024

Hormones

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ENO1 contributes to 5-fluorouracil resistance in colorectal cancer cells via EMT pathway

Cited by 6 publications

References 46 publications

Multiomics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy

Multiomics reveals the role of ENO1 in bladder cancer and constructs an epithelial-related prognostic model to predict prognosis and efficacy

ENO1 deletion potentiates ferroptosis and decreases glycolysis in colorectal cancer cells via AKT/STAT3 signaling

Exploring the role of estrogen and progestins in breast cancer: A genomic approach to diagnosis

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