Enniatin B induces expression changes in the electron transport chain pathway related genes in lymphoblastic T-cell line

Alonso-Garrido, Manuel; Escrivá, Laura; Manyes, Lara; Font, Guillermina

doi:10.1016/j.fct.2018.09.018

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…In mammalian systems, the toxicity of ENN B has been found to increase by co-occurrence, in vitro, with other mycotoxins, depending on the concentrations tested [66]. Enniatins are known to form ion pores in cellular membranes and they can disrupt electrochemical potential of the electron transport chain in the mitochondria, thereby downregulating ATP production, which is required to drive the export of toxins from plant host cells [67]. Thus, the presence of enniatins inhibiting toxin export could increase the susceptibility of plant host cells to other mycotoxins.…”

Section: Discussionmentioning

confidence: 99%

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

et al. 2020

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Fusarium avenaceum is a generalist pathogen responsible for diseases in numerous crop species. The fungus produces a series of mycotoxins including the cyclohexadepsipeptide enniatins. Mycotoxins can be pathogenicity and virulence factors in various plant–pathogen interactions, and enniatins have been shown to influence aggressiveness on potato tubers. To determine the role of these mycotoxins in other F. avenaceum–host interactions, ENNIATIN SYNTHASE 1 (ESYN1) disruption and overexpression mutants were generated and their ability to infect wheat and peas investigated. As a preliminary study, the transformants were screened for their ability to cause potato tuber necrosis and, consistent with a previous report, enniatin production increased necrotic lesion size on the tubers. By contrast, when the same mutants were assessed in their ability to cause disease in pea roots or durum wheat spikes, no changes in disease symptoms or virulence were observed. While it is known that, at least in the case of wheat, exogenously applied enniatins can cause tissue necrosis, this group of mycotoxins does not appear to be a key factor on its own in disease development on peas or durum wheat.

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Section: Discussionmentioning

confidence: 99%

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

et al. 2020

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“…The superposition rates of compound 5b with iodoacetic acid and sodium phosphate were 44.38% and 55.63%, respectively, which indicated that compound 5b had some inhibitory effects on EMP and HMP pathways, but the superposition rates of compound 5b with malonic acid were relatively lower (34.69%), indicating that 5b had more obvious inhibitory effects on the TCA pathway. It has been reported that TCA pathway is related to electron transport. , Therefore, compound 5b may inhibit the electron transport by binding to the Qo site of the Cyt bc complex, thus inhibiting the respiratory function of B. cinerea and achieving the antifungal effect. , …”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that TCA pathway is related to electron transport. 35,36 Therefore, compound 5b may inhibit the electron transport by binding to the Qo site of the Cyt bc complex, thus inhibiting the respiratory function of B. cinerea and achieving the antifungal effect. 37,38 QSAR.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Antifungal Application of Rosin Derivatives from Renewable Pine Resin in Crop Protection

Pan

Jiang

et al. 2020

J. Agric. Food Chem.

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In the current work, we synthesized two series of dehydroabietyl amide derivatives from natural product rosin and evaluated their antifungal effects on Valsa mali, Phytophthora capsici, Botrytis cinerea, Sclerotinia sclerotiorum, and Fusarium oxysporum. In vitro and in vivo antifungal activities results indicated that rosin-based amide compounds containing thiophene heterocycles had better inhibitory effects on B. cinerea. In particular, compound 5b (5-fluoro-2-thiophene dehydroabietyl amide) exhibited the excellent antifungal properties against B. cinerea with an EC 50 of 0.490 mg/L, which was lower compared to the positive control penthiopyrad (0.562 mg/L). Physiological and biochemical studies showed that the primary action mechanism of compound 5b on B. cinerea changes mycelial morphology, increases cell membrane permeability, and inhibits the TCA pathway in respiratory metabolism. Furthermore, QSAR and SAR studies revealed that charge distribution of rosin-based amides derivatives have a key role in the antifungal activity through the hydrogen bonding, conjugation, and electrostatic interaction between the compounds and the receptors of the target. To sum up, this study contributes to the development of rosin-based antifungal agents with a novel structure and preferable biological activity.

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“…Regarding toxicogenomic effects, Alonso-Garrido et al [22] investigated changes in the gene expression profile induced by enniatin B exposure at concentrations of 1.5, 3 and 5 μM to human Jurkat lymphoblastic T-cells after 24 h and observed that 245 genes were differentially expressed and that mitochondria were the organelles with more related differentially expressed genes, that were involved in molecular functions and pathways related to mitochondrial metabolism and cell respiration.…”

Section: Sift Deskmentioning

confidence: 99%

Emerging mycotoxins in botanicals: benefit and risk

Ferrer¹,

Pallarés²,

Font³

et al. 2020

SDRP JFST

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Background: Fusarium species are responsible for production of emerging mycotoxins with cyclic hexadepsispeptides structures, like enniatins and beauvericin. Although these mycotoxins have not been regulated yet, their high prevalence in food and feed, as well as their potential toxic effects in humans and animals have made of them a burning issue and a threat to food security. Besides its inophoric properties, these mycotoxins may induce cells damages such as oxidative stress, mitochondrial modifications and the disruption on the cell cycle related to several health adverse effects such as immunotoxicity, genotoxicity, endocrine toxicity and neurotoxicity. Moreover, they showed interesting activity against various microorganisms and insects in several studies, leading to a potential use in pesticide and medicine research, as potential candidates for anticancer therapy. Botanicals can go contaminated by mycotoxins when the harvesting practices or manufacturing conditions are inadequate, which make mandatory their study. Methods: This review explores emerging mycotoxins occurrence in several botanicals forms and discuss their possible prejudicial and beneficial effects. Results: Several researchers have reported mycotoxins occurrence at low incidences and levels in botanicals. Despite emerging mycotoxins toxic effects, beneficial properties have been attributed to these mycotoxins by several authors. These compounds are related to insecticidal activity, antibacterial properties, and antifungal and antiviral activities. Cell organelles or enzyme systems are the targets in its antimicrobial activity. Data collected showed also that emerging mycotoxins are involved in capacity of the multidrug transport protein in human cancer cells modulation and apoptotic cell death induction, several researchers are explaining the possibility to employ them with medicinal purpose. Conclusion: More studies are required to explore enniatins and beauvericin possible applications in medicinal and pesticide research. Furthermore, it is important to highlight that the regulation of emerging mycotoxins in food must be revised and updated. Keywords: anticancer, antimicrobials, beauvericin, cytotoxicity, enniatins, medicinal plants.

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Enniatin B induces expression changes in the electron transport chain pathway related genes in lymphoblastic T-cell line

Cited by 20 publications

References 39 publications

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

Enniatin Production Influences Fusarium avenaceum Virulence on Potato Tubers, but not on Durum Wheat or Peas

Antifungal Application of Rosin Derivatives from Renewable Pine Resin in Crop Protection

Emerging mycotoxins in botanicals: benefit and risk

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