Enhancing xylanases production by a new Amazon Forest strain of Aspergillus oryzae using solid-state fermentation under controlled operation conditions

Pirota, Rosangela Donizete Perpetua Buzon; Tonelotto, Mariana; Delabona, Priscila da Silva; Fonseca, R. F.; Paixão, Douglas Antônio Alvaredo; Baleeiro, Flávio C. F.; Neto, V. Bertucci; Farinas, Cristiane S.

doi:10.1016/j.indcrop.2013.01.010

Cited by 48 publications

(27 citation statements)

References 36 publications

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“…The culture was maintained on PDA slants at 32 C for 5 days before inoculation for SSF cultivation studies. The identification of the selected strain was performed by extraction of the genomic DNA and amplification of the Internal Transcribed Spacer (ITS) regions, using the same procedure described in a previous work (Pirota et al, 2013). The sequence of the ITS region corresponding to rDNA was submitted for registration at the National Center for Biotechnology Information (NCBI) and received the access number JX163919.…”

Section: Identification Of the Selected Strainmentioning

confidence: 99%

“…Lower enzyme production under static conditions is probably related to oxygen limitation during fungal growth, since O 2 is transferred mainly by diffusion. Besides oxygen transfer, heat transfer and CO 2 dissipation are also favored under dynamic conditions of forced aeration (Pirota et al, 2013). …”

Section: Effect Of Initial Moisture Content On Cellulase Production Bmentioning

confidence: 99%

“…Endoglucanase, -glucosidase, and FPase activities were up to 87, 281, and 210% higher, respectively, using forced aeration. This can be explained by the more favorable environmental conditions encountered in the bioreactor system, such as efficient heat dissipation and avoidance of temperature gradients caused by the heat generated during microbial activity (Pirota et al, 2013).…”

Section: Effect Of Temperature On Cellulase Production By a Oryzaementioning

confidence: 99%

“…The enzymes considered include -amylase (Bogar et al, 2002;Farid and Shata, 2011;Kareem et al, 2009;Pengthamkeerati et al, 2012;Sivaramakrishnan et al, 2007;Xu et al, 2008), protease (Chutmanop et al, 2008), and xylanase (Pirota et al, 2013;Szendefy et al, 2006), amongst others. However, to the best of our knowledge, there have been only a few studies concerning the production of cellulase by A. oryzae cultivated under SSF, from the bioprocess development point of view (Begum and Alimon, 2011;Lio and Wang, 2012;Sandhu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Pirota

Tonelotto

Delabona

et al. 2016

Braz. J. Chem. Eng.

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-Bioprocess development studies concerning the production of cellulases are of crucial importance due to the significant impact of these enzymes on the economics of biomass conversion into fuels and chemicals. This work evaluates the effects of solid-state fermentation (SSF) operational conditions on cellulase production by a novel strain of Aspergillus oryzae using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The use of SSF cultivation under controlled conditions substantially improved cellulase production. Highest production of FPase (0.40 IU g -1 ), endoglucanase (123.64 IU g -1 ), and -glucosidase (18.32 IU g -1 ) was achieved at 28 C, using an initial substrate moisture content of 70%, with an inlet air humidity of 80% and an airflow rate of 20 mL min -1 . Further studies of kinetic profiles and respirometric analyses were performed. The results showed that these data could be very useful for bioprocess development of cellulase production and scale-up.

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Section: Identification Of the Selected Strainmentioning

confidence: 99%

Section: Effect Of Initial Moisture Content On Cellulase Production Bmentioning

confidence: 99%

Section: Effect Of Temperature On Cellulase Production By a Oryzaementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Pirota

Tonelotto

Delabona

et al. 2016

Braz. J. Chem. Eng.

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“…Para isso, a matéria-prima é hidrolisada por um complexo enzimático constituído por três classes principais de enzimas:as endoglucanases, as exoglucanases e as β-glicosidases, que atuamsinergicamente (Zhanget al, 2006). As xilanases atuam como enzimas acessórias, desestruturando o entrelaçamento da hemicelulose presente na parede vegetal, facilitando o acesso à celulose (Dodd e Cann, 2009).A aplicação de xilanases em conjunto com enzimas celulolíticas tem sido amplamente considerado para a bioconversão de materiais lignocelulósicos (Pirota et al, 2013).…”

Section: Introductionunclassified

Estudo Comparativo Da Termoestabilidade De Enzimas Produzidas Por Fungos Filamentosos Em Cultivo Submerso E Combinado

Tardioli¹,

Vasconcellos²,

Florencio³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A termoestabilidade é uma das características que influenciam a eficiência dos complexos enzimáticos, sendo o parâmetro tempo de meia vida utilizado para avaliar tal propriedade. Celulases e xilanases termoestáveis apresentam vantagens na aplicação industrial.Neste trabalho comparou-se a termoestabilidade de extratos enzimáticos produzidos por dois métodos de cultivos (submerso e combinado) e três linhagens fúngicas (Aspergillus niger, Trichoderma harzianum e Trichoderma sp INPA 666), possibilitando a determinação do tempo de meia vida para as enzimas endoglucanases e xilanases a 50°C. Os tempos de meia vida obtidos diferiram entre si quanto ao método de cultivo e fungo. Para endoglucanases, os extratos do cultivo submerso se mostraram mais termoestáveis, destacando-se o extrato do A. niger com o tempo de meia vida de 156 min. Para xilanases, o cultivo combinado do T. harzianum resultou em uma maior termoestabilidade, com tempo de meia vida de 383 min.

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Extremophiles for Hydrolytic Enzymes Productions: Biodiversity and Potential Biotechnological Applications

Kour

Rana

Kaur

et al. 2019

Bioprocessing for Biomolecules Production

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Enhancing xylanases production by a new Amazon Forest strain of Aspergillus oryzae using solid-state fermentation under controlled operation conditions

Cited by 48 publications

References 36 publications

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Estudo Comparativo Da Termoestabilidade De Enzimas Produzidas Por Fungos Filamentosos Em Cultivo Submerso E Combinado

Extremophiles for Hydrolytic Enzymes Productions: Biodiversity and Potential Biotechnological Applications

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