Enhancing transgenic pea (
            <i>Pisum sativum L.</i>
            ) resistance against fungal diseases through stacking of two antifungal genes (Chitinase and Glucanase)

Amian, Awah Anna; Papenbrock, Jutta; Jacobsen, Hans‐Jörg; Hassan, Fathi

doi:10.4161/gmcr.2.2.16125

Cited by 57 publications

(31 citation statements)

References 35 publications

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“…Transgenic pea lines expressing chitinase and/or β1,3-glucanase are also under evaluation for their usefulness against other fungal diseases (Hassan et al, 2009;Amian et al, 2011). There is good potential for transformation to help tackle recalcitrant plant pathogens.…”

Section: G Genetic Transformationmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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Section: G Genetic Transformationmentioning

confidence: 99%

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Rubiales

Fondevilla

Cao

et al. 2014

Critical Reviews in Plant Sciences

176

132

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“…Future work such as deploying antifungal genes like chitinase and glucanase for effective control of biotrophic fungal diseases in transgenic crop plants (Amian et al 2011;Ceasar and Ignacimuthu 2012), or polygalacturonase-inhibitory proteins (PGIPs) to inhibit the activity of the fungal cell wall-degrading polygalacturonases and hence reduced disease symptoms due to necrotrophic pathogens like B. cinerea (Wally and Punja 2010) could make genetic transformation a potentially valuable and practical strategy for breeding resistance to biotic stresses in chickpea. A more efficient way of enhancing and broadening resistance of plants to different biotic stresses is to combine transgenes expressing several genes into a single line using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and IRES elements.…”

Section: Genetic Transformationmentioning

confidence: 99%

Breeding for biotic stress resistance in chickpea: progress and prospects

Li¹,

Rodda²,

Gnanasambandam

et al. 2015

Euphytica

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Chickpea (Cicer arietinum L.) is the third most economically important food legume in the world. Its yield potential is often limited by various biotic stresses, including fungal and viral diseases, insects, nematodes and parasitic weeds. Incorporating genetic resistance into cultivars is the most effective and economical way of controlling biotic stresses and this is a major objective in many breeding programs. Extensive searches for resistances have been conducted by screening commercial varieties, landraces and closely related species. Resistances to disease such as Ascochyta blight and Fusarium wilt have been identified and molecular tools are being used to increase the efficiency of gene transfer from wild species into chickpea elite genotypes. Quantitative trait loci for resistance genes have been located on linkage maps and molecular markers associated with these loci can potentially be used for efficient pyramiding of the traits. Significant chickpea genomic resources have been developed in order to investigate resistance genes. Such resources include an integrated genetic map, expressed sequence tag libraries, bacterial artificial chromosome libraries, microarrays and draft genome sequences. Although these resources have yet to be used to improve chickpea cultivars in the field, this is likely to change in the near future. These genomic resources, as well as high-resolution phenotyping tools and cutting-edge technologies such as next-generation sequencing, promise to increase efficiency as work to identify valuable candidate genes continues.

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“…One of the most widely used strategy involves overexpression of the genes for PR proteins. In some cases, the increased resistance to fungal pathogens was achieved in transgenic plants overexpressing specific chitinase genes (Dana et al, 2006;Amian et al, 2011;Girhepuje and Shinde, 2011;Prasad et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Optimalisation of expression conditions for production of round-leaf sundew chitinase (Drosera rotundifolia L.) in three E. coli expression strains

Rajninec¹,

Libantová²,

Jopčík³

2016

JCEA

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Round-leaf sundew (Drosera rotundifolia L.), family Droseraceae, genus Drosera, is one of a few plant species with a strong antifungal potential. Chitinases of carnivorous plants play an important role in decomposition of chitin-containing cell structures of insect prey. The cell wall of many phytopathogenic fungi also contains chitin, which can be utilized by chitinases, thus round-leaf sundew represents an interesting gene source for plant biotechnology. The purpose of this study was to compare the suitability of 3 different E. coli expression strains (E. coli BL21-CodonPlus® (DE3)-RIPL, E. coli ArcticExpress (DE3)RIL and E. coli SHuffle® T7) for production and isolation of heterologous round-leaf sundew chitinase (DrChit). Results showed that the recombinant protein was successfully expressed in all three strains, but occurred in insoluble protein fraction. To get the DrChit protein into soluble protein fraction some modifications concerning to induction temperatures and concentration of the IPTG inductor were tested. In addition, composition of lysis buffer has been modified with supplementation of strong non-ionic detergents, Triton ® X100 and Tween ® 20, respectively. As these modifications didn't increase the amount of the DrChit protein in soluble fraction, therefore, its isolation under denaturing conditions and subsequent refolding for activity assays is recommended. AbstraktRosička okrúhlolistá (Drosera rotundifolia L.), rodina Droseraceae, rod Drosera, je jednou z mála rastlinných druhov so silným antifungálnym potenciálom. Chitinázy mäsožravých rastlín zohrávajú dôležitú úlohu pri dekompozícií bunkových štruktúr obsahujúcih chitín z tela chyteného hmyzu. Bunkové steny viacerých fytopatogénnych húb obsahujú chitín, ktorý môže byť využívaný chitinázami, čo robí z rosičky okrúhlolistej zaujímavý genetický zdroj pre rastlinné biotechnológie. Cieľom práce bolo porovnanie vhodnosti použitia 3 rozdielnych expresných kmeňov E. coli (E. coli BL21-CodonPlus® (DE3)-RIPL, E. coli ArcticExpress (DE3)RIL a E. coli SHuffle® T7) pre produkciu a izoláciu heterológnej chitinázy rosičky okrúhlolistej (DrChit). Výsledky ukázali, že rekombinantný proteín bol úspešne exprimovaný vo všetkých 3 kmeňoch, ale vyskytoval sa v nerozpustnej proteínovej frakcii. Aby sa DrChit proteín dostal rozpustnej proteínovej frakcie, boli testované modifikácie teploty indukcie a koncentrácie induktora IPTG. Ďalej bolo modifikované zloženie lyzačného pufru pridaním silných neiónových detergentov, Triton ® X100 a Tween ® 20. Pretože tieto modifikácie nezvýšili množstvo získaného DrChit proteínu v rozpustnej frakcii, je odporúčaná jeho izolácia za denaturačných podmienok a následná obnova štruktúry proteínu.

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Enhancing transgenic pea ( Pisum sativum L. ) resistance against fungal diseases through stacking of two antifungal genes (Chitinase and Glucanase)

Cited by 57 publications

References 35 publications

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Achievements and Challenges in Legume Breeding for Pest and Disease Resistance

Breeding for biotic stress resistance in chickpea: progress and prospects

Optimalisation of expression conditions for production of round-leaf sundew chitinase (Drosera rotundifolia L.) in three E. coli expression strains

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