Enhancing the Thermo-Stability and Anti-Bacterium Activity of Lysozyme by Immobilization on Chitosan Nanoparticles

Wang, Yanan; Li, Shangyong; Jin, Man-Wen; Han, Qi; Liu, Songshen; Chen, Xuehong; Han, Yantao

doi:10.3390/ijms21051635

Cited by 31 publications

(8 citation statements)

References 39 publications

(39 reference statements)

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“…The lysozyme is a potential candidate, as it is a biomolecule with key defensive roles in the innate immune system widely distributed in phages, bacteria, plants, vertebrates, and humans [ 65 ]. Owing to its antibacterial, antiviral, and anti-inflammatory properties, it has been extensively used in the medical, environmental protection, and food industries [ 35 , 65 , 66 , 67 ]. The lysozyme’s antimicrobial activity mainly relies on the degradation of the microbial cell wall through the peptidoglycan hydrolyzation, specifically the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine monosaccharides [ 35 , 65 , 66 , 67 , 68 ].…”

Section: Microbial Targeting Strategiesmentioning

confidence: 99%

“…Owing to its antibacterial, antiviral, and anti-inflammatory properties, it has been extensively used in the medical, environmental protection, and food industries [35,[65][66][67]. The lysozyme's antimicrobial activity mainly relies on the degradation of the microbial cell wall through the peptidoglycan hydrolyzation, specifically the β-1,4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine monosaccharides [35,[65][66][67][68]. Furthermore, there is evidence of lysozymal antiviral character based on its potential activity against the human immunodeficiency virus [65].…”

Section: Proteinsmentioning

confidence: 99%

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Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

et al. 2021

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Despite the many advancements in the pharmaceutical and medical fields and the development of numerous antimicrobial drugs aimed to suppress and destroy pathogenic microorganisms, infectious diseases still represent a major health threat affecting millions of lives daily. In addition to the limitations of antimicrobial drugs associated with low transportation rate, water solubility, oral bioavailability and stability, inefficient drug targeting, considerable toxicity, and limited patient compliance, the major cause for their inefficiency is the antimicrobial resistance of microorganisms. In this context, the risk of a pre-antibiotic era is a real possibility. For this reason, the research focus has shifted toward the discovery and development of novel and alternative antimicrobial agents that could overcome the challenges associated with conventional drugs. Nanotechnology is a possible alternative, as there is significant evidence of the broad-spectrum antimicrobial activity of nanomaterials and nanoparticles in particular. Moreover, owing to their considerable advantages regarding their efficient cargo dissolving, entrapment, encapsulation, or surface attachment, the possibility of forming antimicrobial groups for specific targeting and destruction, biocompatibility and biodegradability, low toxicity, and synergistic therapy, polymeric nanoparticles have received considerable attention as potential antimicrobial drug delivery agents. In this context, the aim of this paper is to provide an up-to-date overview of the most recent studies investigating polymeric nanoparticles designed for antimicrobial therapies, describing both their targeting strategies and their effects.

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Section: Microbial Targeting Strategiesmentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

et al. 2021

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“…In a search for greater stability, solubility, and reusability, synthetic conjugates or nanoparticles have been developed, demonstrating improved activity against PA and AB (Liu et al ., 2013c ; Saito et al ., 2019 ). This is particularly interesting for lysozyme immobilized in chitosan nanoparticles through ionic gelation, which is very active against KP, with MICs around 10 mg/l and significant bactericidal capacity (a 2 log unit decrease in viable bacteria in 120 min) (Wu et al ., 2017a ; Wang et al ., 2020 ). Although lysozyme barely inhibits PA biofilm formation, application against KP seems exploitable, since concentrations of 5 mg/l completely eradicated 24 h‐old biofilms (Sheffield et al ., 2012 ; Hukić et al ., 2018 ).…”

Section: Bacterial Envelope‐targeting Mammalian Humoral Innate Immuni...mentioning

confidence: 99%

Mammals' humoral immune proteins and peptides targeting the bacterial envelope: from natural protection to therapeutic applications against multidrug‐resistant Gram‐negatives

et al. 2022

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Mammalian innate immunity employs several humoral ‘weapons’ that target the bacterial envelope. The threats posed by the multidrug‐resistant ‘ESKAPE’ Gram‐negative pathogens (Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) are forcing researchers to explore new therapeutic options, including the use of these immune elements. Here we review bacterial envelope‐targeting (peptidoglycan and/or membrane‐targeting) proteins/peptides of the mammalian immune system that are most likely to have therapeutic applications. Firstly we discuss their general features and protective activity against ESKAPE Gram‐negatives in the host. We then gather, integrate, and discuss recent research on experimental therapeutics harnessing their bactericidal power, based on their exogenous administration and also on the discovery of bacterial and/or host targets that improve the performance of this endogenous immunity, as a novel therapeutic concept. We identify weak points and knowledge gaps in current research in this field and suggest areas for future work to obtain successful envelope‐targeting therapeutic options to tackle the challenge of antimicrobial resistance.

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“…Lysozyme [LYS (L)], a hydrolase widely present in egg whites, has attracted extensive attention within the food industry since the twenty-first century ( 19 ). The LYS has a great potential for food preservation owing to its stability over a wide pH and a temperature range ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Gum Tragacanth-Sodium Alginate Active Coatings Incorporated With Epigallocatechin Gallate and Lysozyme on the Quality of Large Yellow Croaker at Superchilling Condition

Pei

Mei

et al. 2022

Front. Nutr.

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This research was done to investigate the synergistic interactions of the gum tragacanth (GT)–sodium alginate (SA) active coatings, incorporated with epigallocatechin gallate and lysozyme, on the quality of large yellow croaker (Larimichthys crocea) during superchilling storage at −3°C. Results showed that the GT-SA active coatings, containing epigallocatechin gallate [EGCG (E), 0.32% w/v], and lysozyme [LYS (L), 0.32% w/v] have reduced the total viable count, psychrophilic bacteria, and Pseudomonas spp. by about 1.55 log CFU/g, 0.49 log CFU/g, and 1.64 log CFU/g compared to the control at day 35. The GT-SA active coatings containing EGCG and LYS were effective in lowering the formations of off-odor compounds such as total volatile basic nitrogen (TVB-N), malondialdehyde (MDA), and off-favor amino acid (histidine). The solid phase microextraction gas chromatography-mass spectrometer (SPME-GC/MS) was applied to characterize and to quantify the volatile compounds of large yellow croaker samples during superchilling storage, while the relative content of the fishy flavor compounds (including 1-octen-3-ol and acetoin) was significantly reduced in the active coatings treated samples. Furthermore, the GT-SA active coatings containing EGCG and LYS treatments was found to be more effective in retarding the migration of water based on magnetic resonance imaging (MRI) results and in maintaining the organoleptic quality of large yellow croaker in superchilling storage at −3°C according to the sensory evaluation results. The results showed that the GT-SA active coating containing EGCG and LYS was effective to be used as a fish preservative to improve the quality and to prolong the shelf life of large yellow croaker in a superchilling storage for at least 7 days.

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Enhancing the Thermo-Stability and Anti-Bacterium Activity of Lysozyme by Immobilization on Chitosan Nanoparticles

Cited by 31 publications

References 39 publications

Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

Polymeric Nanoparticles for Antimicrobial Therapies: An up-to-date Overview

Mammals' humoral immune proteins and peptides targeting the bacterial envelope: from natural protection to therapeutic applications against multidrug‐resistant Gram‐negatives

Effect of Gum Tragacanth-Sodium Alginate Active Coatings Incorporated With Epigallocatechin Gallate and Lysozyme on the Quality of Large Yellow Croaker at Superchilling Condition

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