Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis

Adeyinka, Olawale Samuel; Tabassum, Bushra; Landry, Koloko Brice; Ogungbe, Ifedayo Victor

doi:10.1007/s00425-023-04110-6

Cited by 7 publications

(1 citation statement)

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“…The expanding CRISPR toolbox enables gene knockout, gene deletion, base editing, gene insertion or replacement, targeted random mutagenesis, epigenetic modulation, transcriptional regulation, and RNA editing [12][13][14]. The CRISPR/Cas system iterative upgrades have provided unprecedented opportunities for gene function analyses and the creation of desirable germplasms in different crops, which will lead to the third agricultural green revolution [15,16]. The combination of CRISPR/Cas technology with traditional breeding methods, molecular marker-assisted selection, double haploid technique, male or female sterility, and asexual reproduction will greatly promote crop breeding, In this review, we mainly summarize the application of CRISPR/Cas technology in gene function analysis, the generation of new germplasms, crop de novo domestication, decoupling the tradeoff effect, conventional hybrid seed production, asexual reproduction, and double haploid breeding [17].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas Technology Revolutionizes Crop Breeding

Tang,

Wang,

Jin

et al. 2023

Plants

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Crop breeding is an important global strategy to meet sustainable food demand. CRISPR/Cas is a most promising gene-editing technology for rapid and precise generation of novel germplasm and promoting the development of a series of new breeding techniques, which will certainly lead to the transformation of agricultural innovation. In this review, we summarize recent advances of CRISPR/Cas technology in gene function analyses and the generation of new germplasms with increased yield, improved product quality, and enhanced resistance to biotic and abiotic stress. We highlight their applications and breakthroughs in agriculture, including crop de novo domestication, decoupling the gene pleiotropy tradeoff, crop hybrid seed conventional production, hybrid rice asexual reproduction, and double haploid breeding; the continuous development and application of these technologies will undoubtedly usher in a new era for crop breeding. Moreover, the challenges and development of CRISPR/Cas technology in crops are also discussed.

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