Enhancing the lipid productivity of yeasts with trace concentrations of iron nanoparticles

Pádrová, Karolína; Čejková, Alena; Cajthaml, Tomáš; Kolouchová, Irena; Vítová, Milada; Sigler, Karel; Řezanka, Tomáš

doi:10.1007/s12223-015-0442-7

Cited by 10 publications

(2 citation statements)

References 36 publications

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“…To the best of our knowledge, no research has been conducted in Schizochytrium sp. and Fe limited conditions; however, several studies on oleaginous fungi indicate a positive relationship between Fe and lipogenesis [70,71] due to its role in the synthesis of citrate, which serves as substrate for acetyl-CoA a precursor for fatty acid synthesis [64,68].…”

Section: Effect Of the Replacement Of Micronoutrients With Pre-treate...mentioning

confidence: 99%

Use of Biofuel Industry Wastes as Alternative Nutrient Sources for DHA-Yielding Schizochytrium limacinum Production

et al. 2020

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The simultaneous use of crude glycerol and effluent from anaerobic digestate, both wastes derived from the biofuel industry, were tested in the frame of circular economy concept, as potential low-cost nutrient sources for the cultivation of rich in docosahexaenoic acid (DHA) oil microalgae strain Schizochytrium limacinum SR21. Initially, the optimal carbon and nitrogen concentration levels for high S. limacinum biomass and lipids production were determined, in a culture media containing conventional, high cost, organic nitrogen sources (yeast extract and peptone), micronutrients and crude glycerol at varying concentrations. Then, the effect of a culture media composed of crude glycerol (as carbon source) and effluent digestate at varying proportions on biomass productivity, lipid accumulation, proximate composition, carbon assimilation and fatty acid content were determined. It was shown that the biomass and total lipid content increased considerably with varying effluent concentrations reaching 49.2 g L−1 at 48% (v/v) of effluent concentration, while the lipid yield at the same effluent concentration reached 10.15 g L−1, compared to 17.0 g L−1 dry biomass and 10.2 g L−1 lipid yield when yeast extract and peptone medium with micronutrients was used. Compared to the control treatment, the above production was obtained with 48% less inorganic salts, which are needed for the preparation of the artificial sea water. It was shown that Schizochytrium limacinum SR21 was able to remediate 40% of the total organic carbon content of the biofuel wastes, while DHA productivity remained at low levels with saturated fatty acids comprising the main fraction of total fatty acid content. The results of the present study suggest that the simultaneous use of two waste streams from the biofuel industry can serve as potential nutrient sources for the growth of Schizochytrium limacinum SR21, replacing the high cost organic nutrients and up to one half the required artificial sea water salts, but upregulation of DHA productivity through optimization of the abiotic environment is necessary for industrial application, including aqua feed production.

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Section: Effect Of the Replacement Of Micronoutrients With Pre-treate...mentioning

confidence: 99%

Use of Biofuel Industry Wastes as Alternative Nutrient Sources for DHA-Yielding Schizochytrium limacinum Production

et al. 2020

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“…Then, although the high reducibility of nanoFe makes it quickly quench ROS caused by inflammation, it tends to be transformed into Fe 2+ after releasing electrons. Subsequently, Fe 2+ can convert less reactive hydrogen peroxide into more ROS, which may be contrary to the original intention of the treatment [ 50 ]. Fe 2+ or Fe 3+ traversed from nanoFe may coordinate with biomolecules to some extent, resulting in adverse effects such as agglomeration.…”

Section: Discussionmentioning

confidence: 99%

Protection of zero-valent iron nanoparticles against sepsis and septic heart failure

Wang

Liang

et al. 2022

J Nanobiotechnol

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Background Septic heart failure accounts for high mortality rates globally. With a strong reducing capacity, zero-valent iron nanoparticles (nanoFe) have been applied in many fields. However, the precise roles and mechanisms of nanoFe in septic cardiomyopathy remain unknown. Results NanoFe was prepared via the liquid-phase reduction method and functionalized with the biocompatible polymer sodium carboxymethylcellulose (CMC). We then successfully constructed a mouse model of septic myocardial injury by challenging with cecal ligation and puncture (CLP). Our findings demonstrated that nanoFe has a significant protective effect on CLP-induced septic myocardial injury. This may be achieved by attenuating inflammation and oxidative stress, improving mitochondrial function, regulating endoplasmic reticulum stress, and activating the AMPK pathway. The RNA-seq results supported the role of nanoFe treatment in regulating a transcriptional profile consistent with its role in response to sepsis. Conclusions The results provide a theoretical basis for the application strategy and combination of nanoFe in sepsis and septic myocardial injury. Graphical Abstract

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Ecotoxicity of Nanometals: The Problems and Solutions

Shurygina

Соседова

Новиков

et al. 2018

Nanomaterials: Ecotoxicity, Safety, and Public Perception

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Enhancing the lipid productivity of yeasts with trace concentrations of iron nanoparticles

Cited by 10 publications

References 36 publications

Use of Biofuel Industry Wastes as Alternative Nutrient Sources for DHA-Yielding Schizochytrium limacinum Production

Use of Biofuel Industry Wastes as Alternative Nutrient Sources for DHA-Yielding Schizochytrium limacinum Production

Protection of zero-valent iron nanoparticles against sepsis and septic heart failure

Ecotoxicity of Nanometals: The Problems and Solutions

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