Enhancing the electrocatalytic activity of palladium nanocluster tags by selective introduction of gold atoms: Application for a wound infection biomarker detection

Toyos-Rodríguez, Celia; Adawy, Alaa; García‐Alonso, Francisco Javier; Escosura‐Muñiz, Alfredo de la

doi:10.1016/j.bios.2021.113926

Cited by 7 publications

(4 citation statements)

References 72 publications

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“…To overcome these problems, nanomaterials have emerged as alternative, both alone or in combination with enzymes [77]. Again, metallic nanoparticles such as silver nanoparticles, gold nanoparticles, bimetallic nanoparticles, or zinc or cerium oxide nanoparticles stand out for their redox properties and electrocatalytic activities [78][79][80].…”

Section: Nanomaterials In the Screening Of Antibiotic Compounds: Are ...mentioning

confidence: 99%

Advances in the screening of antimicrobial compounds using electrochemical biosensors: is there room for nanomaterials?

2022

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The abusive use of antimicrobial compounds and the associated appearance of antimicrobial resistant strains are a major threat to human health. An improved antimicrobial administration involves a faster diagnosis and detection of resistances. Antimicrobial susceptibility testing (AST) are the reference techniques for this purpose, relying mainly in the use of culture techniques. The long time required for analysis and the lack of reproducibility of these techniques have fostered the development of high-throughput AST methods, including electrochemical biosensors. In this review, recent electrochemical methods used in AST have been revised, with particular attention on those used for the evaluation of new drug candidates. The role of nanomaterials in these biosensing platforms has also been questioned, inferring that it is of minor importance compared to other applications. Graphical Abstract

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Section: Nanomaterials In the Screening Of Antibiotic Compounds: Are ...mentioning

confidence: 99%

Advances in the screening of antimicrobial compounds using electrochemical biosensors: is there room for nanomaterials?

2022

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“…Hybrid Pd-AuNCs have been used as tags for the detection of hyaluronidase wound infection biomarker in a magnetic bead-based immunoassay. [51] However, to the best of our knowledge, the catalytic activity of PdNCs has never been used as the basis of the detection of a LAMP amplification of genetic material. MNPs have been used in the detection of genetic material after RT-LAMP amplification, [52] but their catalytic effect has not been the basis of the detection.…”

Section: Introductionmentioning

confidence: 99%

“…Hybrid Pd‐AuNCs have been used as tags for the detection of hyaluronidase wound infection biomarker in a magnetic bead‐based immunoassay. [ 51 ]…”

Section: Introductionmentioning

confidence: 99%

Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS‐CoV‐2 by Reverse Transcription Loop‐Mediated Isothermal Amplification

Rodríguez-Penedo

Rioboó-Legaspi

González-López

et al. 2023

Adv Healthcare Materials

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Quantitative polymerase chain reaction (qPCR) is considered the gold standard for pathogen detection. However, improvement is still required, especially regarding the possibilities of decentralization. Apart from other reasons, infectious diseases demand on‐site analysis to avoid pathogen spreading and increase treatment efficacy. In this paper, the detection of SARS‐CoV‐2 is carried out by reverse transcription loop‐mediated isothermal amplification, which has the advantage of requiring simple equipment, easily adaptable to decentralized analysis. It is proposed, for the first time, the use of palladium nanoclusters (PdNCs) as indicators of the amplification reaction at end point. The pH of the medium decreases during the reaction and, in turn, a variation in the catalytic activity of PdNCs on the oxygen reduction reaction (ORR) can be electrochemically observed. For the detection, flexible and small‐size screen‐printed electrodes can be premodified with PdNCs, which together with the use of a simple and small electrochemical equipment would greatly facilitates their integration in field‐deployable devices. This would allow a faster detection of SARS‐CoV‐2 as well as of other future microbial threats after an easy adaptation.

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“…Due to the insensitivity of these mediators to pH, their lower redox potential, and their electrochemically reversible nature, these mediators with outer-sphere electron transfer mechanisms became the conventional species in heterogeneous electron transfer reactions in nearly all amperometric biosensors . ORR has also been used recently as the catalytic reaction in electrochemical immunosensors, where nanostructures that are electroactive for the ORR have been employed as labels for signal amplification − and for indirect signal generation in surface (bio)sensors. − …”

Section: Introductionmentioning

confidence: 99%

Utilizing the Oxygen Reduction Reaction in Particle Impact Electrochemistry: A Step toward Mediator-Free Digital Electrochemical Sensors

Moazzenzade,

Huskens,

Lemay

2023

ACS Omega

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The current blockade particle impact method opens a route toward highly parallelized single-entity electrochemical assays. An important limitation is, however, that a redox mediator must be present in the sample, which can detrimentally interfere with molecular recognition processes. Dissolved O2 that is naturally present in aqueous solutions under ambient conditions can in principle serve as a suitable mediator via the oxygen reduction reaction (ORR). Here, we demonstrate the validity of this concept by performing current blockade experiments to capture and detect individual microparticles at Pt microelectrodes using solely the ORR. The readout modality is independent of the absolute O2 concentration, allowing operation under varying conditions. We further determine how the trajectories of individual microparticles are influenced by the combination of electrophoresis and electroosmotic flows and how these can be utilized to provide continuous detection of cationic particles in water for environmental monitoring.

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Enhancing the electrocatalytic activity of palladium nanocluster tags by selective introduction of gold atoms: Application for a wound infection biomarker detection

Cited by 7 publications

References 72 publications

Advances in the screening of antimicrobial compounds using electrochemical biosensors: is there room for nanomaterials?

Advances in the screening of antimicrobial compounds using electrochemical biosensors: is there room for nanomaterials?

Electrocatalytic Palladium Nanoclusters as Versatile Indicators of Bioassays: Rapid Electroanalytical Detection of SARS‐CoV‐2 by Reverse Transcription Loop‐Mediated Isothermal Amplification

Utilizing the Oxygen Reduction Reaction in Particle Impact Electrochemistry: A Step toward Mediator-Free Digital Electrochemical Sensors

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