Enhancing the Cellular Delivery of Nanoparticles Using Lipo‐Oligoarginine Peptides

Abstract

Nanoparticles have great potential as nanotherapeutics, delivery vectors, and molecular imaging agents due to their flexible properties. Although intracellular and nuclear delivery of nanoparticles is desirable for therapeutic applications, it remains a challenge. Cell penetrating peptides (CPPs) are a powerful tool for the intracellular delivery of various cargoes. Here we report that functionalization of nanoparticles with a myristoylated oligoarginine CPP promoted cellular uptake without increased toxicity. It was evidenced that the myristoylated CPP is much more effective in transporting nanoparticles than the unmodified CPPs.

Viral and Other Cell-Penetrating Peptides as Vectors of Therapeutic Agents in Medicine

“…The advantages of the covalent bonding for in vivo applications are reproducibility of the procedure as well as control of the stoichiometry of as-prepared conjugates. This strategy was successfully used to internalize into cells uncharged oligonucleotides (ONs) such as PNA and phosphorodiamidate morpholino oligomer (Laufer and Restle, 2008;Moulton and Moulton, 2008;Kenien et al, 2012;Margus et al, 2012) as well as small-molecule drugs (Bolhassani, 2011) and nanoparticles (Delehanty et al, 2010;Lee and Tung, 2012;Chakrabarti et al, 2014).…”

Micro‐ and Nanotechnologies for Intracellular Delivery

“…Despite CPPs exhibiting a promising potential for NPA delivery for quickly penetrating tissues and crossing cellular membranes, they are universal transporters which lack receptor specificity because of their positive charges, thus hindering their practical in vivo application . To address the issue, in the past few years, substantial improvements in designing and implementing CPP‐based NAP delivery systems have been continuously made . In 2012, Bhatia et al constructed a library of tandem CPPs that condense siRNA into stable tumor‐penetrating nanocomplexes through noncovalent interactions for receptor‐specific siRNA delivery .…”

“…[ 65 ] To address the issue, in the past few years, substantial improvements in designing and implementing CPP-based NAP delivery systems have been continuously made. [66][67][68] In 2012, Bhatia et al constructed a library of tandem CPPs that condense siRNA into stable tumor-penetrating nanocomplexes through noncovalent interactions for receptor-specifi c siRNA delivery. [ 69,70 ] Through the assistance of both tumor-specifi c and cell-penetrating peptides, the nanocomplex may deliver siRNA deep into the tumor site with enhanced specifi city.…”

Next-Generation Nanoantibacterial Tools Developed from Peptides

“…Finally, it is important to mention that other nanocarriers have been coated or attached with peptides mainly with the intention of delivery, for example, liposomes [46], gold nanoparticles [47][48][49] and quantum dots [50], among others [29]. Further, Welsher and Yang [51] have recently shown that one can track (three dimensionally) a single particle during nanoparticle internalization using a two-photon laser scanning microscopy.…”