Enhancing the Catalytic Activity of Geranylgeranyl Diphosphate Synthase through Ancestral Sequence Reconstruction and Semirational Design
Zhiwen Wang,
Yunpeng Shen,
Longyun Cao
et al.
Abstract:Geranylgeranyl diphosphate synthase (GGPPS) is the crucial
bottleneck
in carotenoid biosynthesis. However, low activity limits the broad
application of GGPPS. In this study, OsGGPPS1 in rice was engineered
based on ancestral sequence reconstruction (ASR) and semirational
design to improve the catalytic performances of existing GGPPS. The
better mutant of A22R/A26P with improved enzyme activity was generated
based on ASR. Additionally, the improved enzyme activity of mutants
as V162A/M218S/F227Y was designed us… Show more
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