Enhancing surface functionality of reduced graphene oxide biosensors by oxygen plasma treatment for Alzheimer's disease diagnosis

Chae, Myung Sic; Kim, Jinsik; Jeong, Dahye; Kim, Young Soo; Roh, Jee Hoon; Lee, Sung Min; Heo, Youhee; Kang, Ji Yoon; Lee, Jeong Hoon; Yoon, Dae Sung; Kim, Tae Geun; Chang, Suk Tai; Hwang, Kyo Seon

doi:10.1016/j.bios.2016.10.049

Cited by 75 publications

(46 citation statements)

References 35 publications

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“…Given the carbon-based chemistry and outstanding biocompatible properties, graphene oxide (GO) has attracted considerable interest among nanomaterials such as silver, zinc oxide, and magnesium oxide. 4 GO has been explored in a wide range of diagnostic and therapeutic fields, showing potential in areas such as engineered tissues, 5 drug delivery carriers, 6 biomedical imaging, 7 antibacterial materials, 8 Alzheimer's disease diagnosis, 9 and anticancer 10 therapies.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of oxidative stress, apoptosis, and autophagy involved in graphene oxide nanomaterial anti-osteosarcoma effect

Tang

Zhao

Yang

et al. 2018

IJN

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BackgroundGraphene and its derivative graphene oxide (GO) have been implicated in a wide range of anticancer effects.PurposeThe objective of this study was to systematically evaluate the toxicity and underlying mechanisms of GO on two osteosarcoma (OSA) cancer cell lines, MG-63 and K7M2 cells.MethodsMG-63 and K7M2 cells were treated by GO (0–50 µg/mL) for various time periods. Cell viability was tested by MTT and Live/Dead assays. A ROS Detection Kit based on DHE oxidative reaction was used for ROS detection. An Annexin V-FITC Apoptosis Kit was used for apoptosis detection. Dansylcadaverine (MDC) dyeing was applied for seeking unspecific autophagosomes. Western blot and Immunofluorescence analysis were used for related protein expression and location.ResultsK7M2 cells were more sensitive to GO compared with MG-63 cells. The mechanism was attributed to the different extent of the generation of reactive oxygen species (ROS). In K7M2 cells, ROS was easily stimulated and the apoptosis pathway was subsequently activated, accompanied by elevated expression of proapoptosis proteins (such as caspase-3) and decreased expression levels of antiapoptosis proteins (such as Bcl-2). A ROS inhibitor (N-acetylcysteine) could alleviate the cytotoxic effects of GO in K7M2 cells. However, the production of ROS in MG-63 cells was probably inhibited by the activation of an antioxidative factor, nuclear factor-E2-related factor-2, which translocated from the cytoplasm to the nucleus after GO treatment, while a nuclear factor-E2-related factor-2 inhibitor (ML385) significantly increased ROS production in MG-63 cells when combined with GO treatment. In addition, autophagy was simultaneously stimulated by characteristic autophagosome formation, autophagy flux, and increased the expression level of autophagy-related proteins (such as LC3I to LC3II conversion, ATG5, and ATG7).ConclusionThis paper proposes various underlying mechanisms of the anticancer effect of GO. The novel synthetic use of GO with an oxidizing agent is the key step for further potential applications in clinical OSA cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Mechanisms of oxidative stress, apoptosis, and autophagy involved in graphene oxide nanomaterial anti-osteosarcoma effect

Tang

Zhao

Yang

et al. 2018

IJN

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“…The 2D peak in GO and rGO spectra is often accompanied by higher order peak 2D', an overtone of D' mode. The typical G band due to the first order scattering of the E2g mode is observed in all graphene-like samples [22,29]. This band, named here as apparent G app is, however, a superposition of G and D' modes, which is very often neglected in the literature.…”

Section: Surface Chemistry Change After Oxygen Plasma Treatment Of Rgomentioning

confidence: 99%

“…However, the use of O 2 plasma in the treatment of novel carbon nanomaterials is still limited. To the best of our knowledge, there are only few reports showing the effect of O 2 plasma on graphene, carbon nanotubes and rGO [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

“…Favia et al [21] recently demonstrated an efficient method to modify carbon nanotubes by O 2 plasma in order to improve their aqueous dispersions. More recently, Hwang et al [22] modified chemically reduced GO with O 2 plasma and managed to improve its surface reactivity for biomolecular interactions. Despite the above studies, a full understanding of the influence of O 2 plasma treatment on the surface chemistry of rGO is still lacking.…”

Section: Introductionmentioning

confidence: 99%

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Tailoring properties of reduced graphene oxide by oxygen plasma treatment

Kondratowicz

Nadolska

Şahin

et al. 2018

Applied Surface Science

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“…Because the polymers generally characterized by low surface energy and hence weak adhesive properties, investigating a method to enhance the properties of the introduced active layer (ZnO‐NPs:PANI) can be an excellent procedure to get a layer with optimum performance. Meanwhile, the plasma treatment has been used extensively in a variety of branches such as textiles, polymers, biosensors, etc. For example, argon RF plasma treatments showed increasing surface roughness with increasing RF power and treatment time .…”

Section: Introductionmentioning

confidence: 99%

Plasma treatment of zinc oxide‐nanoparticles:polyaniline blend as an active layer for the hybrid bulk heterojunction solar cell applications

2020

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In this experimental work, plasma treatment of the active layer in the bulk heterojunction solar cells was studied. The active layers consisting of zinc oxide nanoparticles:polyaniline were spin-coated on indium tin oxide covered glasses then kept in the cold plasma medium for different treatment times. The J-V characteristics were considered under air mass 1.5G standard illumination, and variations of the open-circuit voltages and short circuit currents were studied under different treatment times. The results show that there is an optimum treatment time to improve the properties of the layers. In order to understand the origin of this effect, the Hall coefficient, along with ultravioletvisible spectra were measured, and for studying the topological impact of plasma on the surface of the layers, atomic force microscopy and Fouriertransform infrared spectroscopy were considered. The measurements confirmed the time dependency of the open-circuit voltages and short circuit currents of the cells on the plasma treatment times. Atomic force microscopy of the layers shows the significant topological effects of the plasma treatments on the surface of the active layers for different treatment times. K E Y W O R D S hybrid solar cells, photovoltaic cells, plasma applications

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Enhancing surface functionality of reduced graphene oxide biosensors by oxygen plasma treatment for Alzheimer's disease diagnosis

Cited by 75 publications

References 35 publications

Mechanisms of oxidative stress, apoptosis, and autophagy involved in graphene oxide nanomaterial anti-osteosarcoma effect

Mechanisms of oxidative stress, apoptosis, and autophagy involved in graphene oxide nanomaterial anti-osteosarcoma effect

Tailoring properties of reduced graphene oxide by oxygen plasma treatment

Plasma treatment of zinc oxide‐nanoparticles:polyaniline blend as an active layer for the hybrid bulk heterojunction solar cell applications

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