Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination

Sánchez-Ortiga, Emilio; Martı́nez-Corral, Manuel; Saavedra, Genaro; Garcı́a-Sucerquia, Jorge

doi:10.1364/ol.39.002086

Cited by 30 publications

(7 citation statements)

References 12 publications

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“…Applications of this have included quantitative measurements of whole-cell morphology and mass, spectroscopy, hemoglobin concentration, etc [8][9][10]. Previous work that considered SI's capabilities to augment the effectiveness and accuracy of these applications has utilized SI for subdiffraction resolution imaging of coherent scatter and optical phase-delays [17,[37][38][39][40]. To the best of our knowledge, this work reports one of the earliest examples of applying the SI framework for extracting 3D biological RI, the intrinsic optical property that determines the object's scattering and phase-delay characteristics [41].…”

Section: Discussionmentioning

confidence: 99%

Refractive index tomography with structured illumination

Chowdhury¹,

Eldridge²,

Wax³

et al. 2017

Optica

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This work introduces a novel reinterpretation of structured illumination (SI) microscopy for coherent imaging that allows three-dimensional imaging of complex refractive index (RI). To do so, we show that coherent SI is mathematically equivalent to a superposition of angled illuminations. It follows that raw acquisitions for standard SI-enhanced quantitative-phase images can be processed into complex electric-field maps describing sample diffraction under angled illuminations. Standard diffraction tomography (DT) computation can then be used to reconstruct the sample's 3D RI distribution at sub-diffraction resolutions. We demonstrate this concept by using a SI-quantitative-phase imaging system to computationally reconstruct 3D RI distributions of human breast (MCF-7) and colorectal (HT-29) adenocarcinoma cells. Our experimental setup uses a spatial light modulator to generate structured patterns at the sample and collects angle-dependent sample diffraction using a common-path, off-axis interference configuration with no moving components. Furthermore, this technique holds promise for easy pairing with SI fluorescence microscopy, and important future extensions may include multimodal, sub-diffraction resolution, 3D RI and fluorescent visualizations.

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Section: Discussionmentioning

confidence: 99%

Refractive index tomography with structured illumination

Chowdhury¹,

Eldridge²,

Wax³

et al. 2017

Optica

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“…Previous works, however, have demonstrated that structured illumination (SI) microscopy, already an established imaging technique for fluorescent super-resolution [29][30][31][32][33][34], has considerable utility as a SA technique when operated in the coherent imaging realm. Such studies have demonstrated SI's coherent sub-diffraction resolution imaging capabilities for visualizing 2D and 3D scattering/quantitative-phase (QP), as endogenous contrast mechanisms [35][36][37][38][39][40][41][42][43]. Hence, SI fills a niche role as a sub-diffraction resolution imaging solution suitable for studies probing molecular and biophysical/biochemical processes.…”

Section: Introductionmentioning

confidence: 99%

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction

Chowdhury¹,

Eldridge²,

Wax³

et al. 2017

Biomed. Opt. Express

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Though structured illumination (SI) microscopy is a popular imaging technique conventionally associated with fluorescent super-resolution, recent works have suggested its applicability towards sub-diffraction resolution coherent imaging with quantitative endogenous biological contrast. Here, we demonstrate that SI can efficiently integrate together the principles of fluorescent super-resolution and coherent synthetic aperture to achieve 3D dual-modality sub-diffraction resolution, fluorescence and refractive-index (RI) visualizations of biological samples. We experimentally demonstrate this framework by introducing a SI microscope capable of 3D sub-diffraction resolution fluorescence and RI imaging, and verify its biological visualization capabilities by experimentally reconstructing 3D RI/fluorescence visualizations of fluorescent calibration microspheres as well as alveolar basal epithelial adenocarcinoma (A549) and human colorectal adenocarcinmoa (HT-29) cells, fluorescently stained for F-actin. This demonstration may suggest SI as an especially promising imaging technique to enable future biological studies that explore synergistically operating biophysical/biochemical and molecular mechanisms at sub-diffraction resolutions.

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“…The lateral resolution is related with the wavelength and the MO's numerical aperture. Therefore, the synthetic aperture method [39][40][41][42][43] and structured illumination approach [44][45][46][47] are utilized to enhance the resolution when the wavelength remains unchanged. In addition, when the specimen's depth change is larger than the optical path of a wavelength, the phase unwrapping algorithms are adopted to obtain the real phase for 2π ambiguity problem [48][49][50], in which multi-wavelength phase unwrapping methods permit larger synthetic wavelengths to unwrap [51][52][53].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

Liu

Wang

2018

Applied Sciences

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Digital holographic microscopy (DHM) is a topographic measurement technique that permits full-field, nondestructive, dynamic, quantitative amplitude, and phase-contrast imaging. The technique may realize the lateral resolution with submicron scale and the longitudinal resolution with subnanometer scale, respectively. Improving imaging quality has always been the research focus in DHM since it has a direct effect on the precise topographic measurement. In this paper, the recent progress on phase aberration compensation and coherent noise suppression is reviewed. Included in this review are the hologram spectrum's centering judgment methods of side band in tilt phase error compensation, the physical and numerical compensation methods in phase aberration compensation, and the single-shot digital process methods in coherent noise suppression. The summaries and analyses for these approaches can contribute to improving the imaging quality and reducing the measurement error of DHM, which will further promote the wider applications of DHM in the topographic measurement fields, such as biology and micro-electro mechanical systems.

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Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination

Cited by 30 publications

References 12 publications

Refractive index tomography with structured illumination

Refractive index tomography with structured illumination

Structured illumination microscopy for dual-modality 3D sub-diffraction resolution fluorescence and refractive-index reconstruction

Recent Progress on Aberration Compensation and Coherent Noise Suppression in Digital Holography

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