Abstract:This study aims to enhance the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) detection and visualization of extracellular polystyrene degrading enzyme expressed by Bacillus megaterium as a result of utilizing polystyrene as a sole carbon source. Dilute polystyrene degrading enzyme expressed in the culture supernatant could not be directly visualized from SDS-PAGE stained with colloidal Coomassie blue stain as it falls below its sensitivity or detection limit level. To overcome this prob… Show more
“…The monooxygenases involved in the alkane hydroxylase system are the main enzymes of interest. There are many distinct alkane hydroxylase numbers and types because different bacteria have varied induction conditions and amounts of target carbon in the alkane chain ( Tan et al, 2022 ).…”
Section: Mechanism Of Plastic Enzymatic Degradationmentioning
“…The monooxygenases involved in the alkane hydroxylase system are the main enzymes of interest. There are many distinct alkane hydroxylase numbers and types because different bacteria have varied induction conditions and amounts of target carbon in the alkane chain ( Tan et al, 2022 ).…”
Section: Mechanism Of Plastic Enzymatic Degradationmentioning
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