Enhancing recombinant antibody performance by optimally engineering its format

Murphy, Caroline; Stack, Edwina; Krivelo, Svetlana; Breheny, M.; Ma, Hui; O’Kennedy, Richard

doi:10.1016/j.jim.2018.10.005

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…Ma et al (2019) demonstrated that compared with scFv, the stability of scAb was greatly improved while the binding activity and affinity were almost unchanged (Ma et al, 2019). The scAb developed by Murphy et al (2018) is more stable than scFv and increases the dynamic range of MC-LR detection (Murphy et al, 2018).…”

Section: Soluble Expression Of Scfv Antibodymentioning

confidence: 96%

“…For example, Sonoda et al (2011) achieved high productivity of functional scFv by co-expressing the periplasmic factors Skp and FkpA (Sonoda et al, 2011). Additionally, the method of incorporating a light chain constant region (CL) at the end of scFv to construct scAb can also improve the stability (Hayes et al, 2012;Ma et al, 2019;Murphy et al, 2018). Ma et al (2019) demonstrated that compared with scFv, the stability of scAb was greatly improved while the binding activity and affinity were almost unchanged (Ma et al, 2019).…”

Section: Soluble Expression Of Scfv Antibodymentioning

confidence: 99%

“…(2011) achieved high productivity of functional scFv by co‐expressing the periplasmic factors Skp and FkpA (Sonoda et al., 2011). Additionally, the method of incorporating a light chain constant region (CL) at the end of scFv to construct scAb can also improve the stability (Hayes et al., 2012; Ma et al., 2019; Murphy et al., 2018). Ma et al.…”

Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

“…The scAb developed by Murphy et al. (2018) is more stable than scFv and increases the dynamic range of MC‐LR detection (Murphy et al., 2018).…”

Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

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Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

et al. 2022

Comp Rev Food Sci Food Safe

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Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) that may be present in food. However, traditional polyclonal and monoclonal antibodies are limited by animal hosts and hinder further development of immunoassays. With the gradual application of phage display technology as an efficient in vitro selection technology, the single‐chain fragment variable (scFv) now provides an exciting alternative to traditional antibodies. Efficiently constructed scFv source libraries and specifically designed biopanning schemes can now yield scFvs possessing specific recognition capabilities. A rational mutation strategy further enhances the affinity of scFv, and allows it to reach a level that cannot be achieved by immunization. Finally, appropriate prokaryotic expression measures ensure stable and efficient production of scFv. Therefore, when developing excellent scFvs, it is necessary to focus on three key aspects of this process that include screening, mutation, and expression. In this review, we analyze in detail the preparation and affinity improvement process for scFv and provide insights into the research progress and development trend of scFv‐based immunoassay methods for monitoring small‐molecule harmful substances.

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Section: Soluble Expression Of Scfv Antibodymentioning

confidence: 96%

Section: Soluble Expression Of Scfv Antibodymentioning

confidence: 99%

Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

“…The scAb developed by Murphy et al. (2018) is more stable than scFv and increases the dynamic range of MC‐LR detection (Murphy et al., 2018).…”

Section: Screening Of Scfv Using Phage Display Technologymentioning

confidence: 99%

See 2 more Smart Citations

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

et al. 2022

Comp Rev Food Sci Food Safe

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“…High selectivity and biomarker recovery can be further enhanced by the improvement of receptor-analyte binding affinities. The dissociation constant between immobilised antibodies and their target antigens is usually known to vary between 10 −7 to 10 −11 M, with some antibodies demonstrating even stronger affinities [212,213]. Beyond the improved capacity to rapidly form stable bonds with the target antigen, receptors with high affinities ensure the antigen will remain bound during the assay and sensor washing steps.…”

Section: Biomimetic Sensors Analytical Performancementioning

confidence: 99%

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Regan

Walsh

O’Kennedy

et al. 2019

Sensors

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Molecular imprinting is a rapidly growing area of interest involving the synthesis of artificial recognition elements that enable the separation of analyte from a sample matrix and its determination. Traditionally, this approach can be successfully applied to small analyte (<1.5 kDa) separation/ extraction, but, more recently it is finding utility in biomimetic sensors. These sensors consist of a recognition element and a transducer similar to their biosensor counterparts, however, the fundamental distinction is that biomimetic sensors employ an artificial recognition element. Molecularly imprinted polymers (MIPs) employed as the recognition elements in biomimetic sensors contain binding sites complementary in shape and functionality to their target analyte. Despite the growing interest in molecularly imprinting techniques, the commercial adoption of this technology is yet to be widely realised for blood sample analysis. This review aims to assess the applicability of this technology for the point-of-care testing (POCT) of cardiovascular disease-related biomarkers. More specifically, molecular imprinting is critically evaluated with respect to the detection of cardiac biomarkers indicative of acute coronary syndrome (ACS), such as the cardiac troponins (cTns). The challenges associated with the synthesis of MIPs for protein detection are outlined, in addition to enhancement techniques that ultimately improve the analytical performance of biomimetic sensors. The mechanism of detection employed to convert the analyte concentration into a measurable signal in biomimetic sensors will be discussed. Furthermore, the analytical performance of these sensors will be compared with biosensors and their potential implementation within clinical settings will be considered. In addition, the most suitable application of these sensors for cardiovascular assessment will be presented.

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Antibody, Aptamer and Affimer-Based Affinity Tools for Marine Toxin Biosensing

Murphy

2023

The Handbook of Environmental Chemistry

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Enhancing recombinant antibody performance by optimally engineering its format

Cited by 9 publications

References 33 publications

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

Evaluation of Molecularly Imprinted Polymers for Point-of-Care Testing for Cardiovascular Disease

Antibody, Aptamer and Affimer-Based Affinity Tools for Marine Toxin Biosensing

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