Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Cour, Rasmus la; Schjøerring, Jan K.; Jørgensen, Henning

doi:10.3389/fsufs.2019.00112

Cited by 19 publications

(20 citation statements)

References 21 publications

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“…Treatment with proteases increased the recovery rate to 80% and previous addition of carbohydrases improved the rate further to 95% (Dotsenko and Lange, 2017). LaCour et al increased the amount of precipitated protein in press juices from ryegrass, red clover, a grassclover mixture, and spinach by adding lignosulfonates from 34-46 to 41-55% (La Cour et al 2019).…”

Section: Extraction Of Proteinsmentioning

confidence: 99%

Material utilization of green waste: a review on potential valorization methods

Langsdorf

Volkmar

Holtmann

et al. 2021

Bioresour. Bioprocess.

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Considering global developments like climate change and the depletion of fossil resources, the use of new and sustainable feedstocks such as lignocellulosic biomass becomes inevitable. Green waste comprises heterogeneous lignocellulosic biomass with low lignin content, which does not stem from agricultural processes or purposeful cultivation and therefore mainly arises in urban areas. So far, the majority of green waste is being composted or serves as feedstock for energy production. Here, the hitherto untapped potential of green waste for material utilization instead of conventional recycling is reviewed. Green waste is a promising starting material for the direct extraction of valuable compounds, the chemical and fermentative conversion into basic chemicals as well as the manufacturing of functional materials like electrodes for electro-biotechnological applications through carbonization. This review serves as a solid foundation for further work on the valorization of green waste.

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Section: Extraction Of Proteinsmentioning

confidence: 99%

Material utilization of green waste: a review on potential valorization methods

Langsdorf

Volkmar

Holtmann

et al. 2021

Bioresour. Bioprocess.

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“…This is owing to the high digestibility, high protein content, and the well-balanced amino acid profile. Moreover, crude protein content in alfalfa leaf protein concentrate is comparable with this measured for soybean almost 45% [29]. Therefore, alfalfa served as a control and soybean was used as a reference feeding model.…”

Section: Introductionmentioning

confidence: 58%

Refining high-quality leaf protein and valuable co-products from green biomass of Jerusalem artichoke (Helianthus tuberosus L.) for sustainable protein supply

Kaszás

Alshaal

Kovács

et al. 2020

Biomass Conv. Bioref.

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The present study evaluates the green biomass of Jerusalem artichoke (JA) as an alternative green protein. A leaf protein concentrate (LPC) was prepared from leafy shoots using biotechnological methods. Seven clones were compared to assess the importance of the genetic basis of JA, and alfalfa served as the control. The LPC content of JA was an average 39 g kg −1 of fresh biomass, while that of alfalfa was 32 g kg −1. The JA can produce up to 936 kg of protein ha −1 year −1 without fertilization under rainfed conditions. The crude protein content of the LPC varied from 24.2 to 31.4 m/m%, depending on clones and harvesting time, which was comparable to that of alfalfa LPC (~32.3%). The amino acid profile of the LPC of JA, particularly of the essential amino acids, was similar to alfalfa and soybean. In addition, our results confirmed that the polyunsaturated fatty acid (PUFA) content varied between 64 and 68% in the LPC fraction, regardless of the clone that was used, with linoleic acid and linolenic acid being the predominant PUFAs. In addition, unlike alfalfa, the content of arachidonic acid was 0.5% in the JA LPC. The tuber yield was significantly reduced because of the repeated harvesting of the shoot parts; however, the tubers obtained were sufficient to regenerate the plantation in the subsequent year, thus ensuring the renewable ability and sustainability of the green biomass of JA.

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“…Previous research has shown promising results on the use of perennial grasses as feedstock for protein extraction (Kromus et al, 2006) with subsequent application as a high-quality substitute for soy. Current estimates indicated that up to 47% of the crude protein (CP) fraction present in the biomass can be extracted in a protein concentrate that may substitute the currently used soybean meal in diets for monogastric animals (Santamaría-Fernández et al, 2017;Stødkilde et al, 2018;la Cour et al, 2019). Furthermore, by-products resulting from the biorefining process, such as the fibre fraction and the brown juice, may additionally be used directly as valuable forage for ruminants (Damborg et al, 2018) or can be further refined into value-added products (Ambye-Jensen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of Harvest and Fertilization Frequency on Protein Yield and Extractability From Flood-Tolerant Perennial Grasses Cultivated on a fen Peatland

Nielsen

Stødkilde

Jørgensen

et al. 2021

Front. Environ. Sci.

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Paludiculture, and in particular the cultivation of perennial grasses as biomass feedstock for green biorefineries, may be an economic and environmentally sustainable option for agricultural peatlands in temperate regions. However, the optimal biomass quality for protein extraction from flood-tolerant grasses is largely unknown. The aim of this study was to define the combined effect of harvest and fertilization frequency, with one to five annual cuts, on protein yield and extractability for the grasses tall fescue (TF) and reed canary grass (RCG), cultivated on an agricultural fen peatland in Denmark.The content of protein fractions was determined according to the Cornell Net Carbohydrate and Protein System (CNCPS). We assessed protein extractability by lab-scale biorefinery techniques using a screw-press followed by acid precipitation of true protein. The two methods were compared to correlate potential extractable protein yields with actual biorefinery outputs. We found the highest annual biomass and crude protein (CP) yields in the two cut treatments, with 13.4 and 15.6 t dry matter (DM) ha−1 year−1, containing 2.9–3.4 t CP ha−1 year−1 for TF and RCG, respectively. The highest neutral-extractable (fractions B1 and B2) true protein yields of 1.1 and 1.5 t ha−1 year−1 were found in the two cut treatments, representing 39% (TF) - 45% (RCG) of total CP. Using biorefining techniques, we were able to precipitate up to 2.2 t DM ha−1 year−1 of protein concentrate, containing up to 39% CP. Significant correlations between methods were found, with a distinct relationship between CNCPS fractions B1 + B2 and CP yield of the protein concentrate, indicating the suitability of the CNCPS as an indicator for extractable protein yields. Biomass and CP yields were not significantly improved beyond two annual cuts. However, timing and harvest frequencies significantly affected plant maturity and consequently extractable CP contents and protein concentrate yields. We conclude that TF and RCG are promising feedstocks for green biorefineries due to high biomass, extractable CP, and protein concentrate yields, and highlight the potential of flood-tolerant grasses, cultivated on wet agricultural peatlands, for an enhanced valorisation beyond the common utilisation for bioenergy.

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Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Cited by 19 publications

References 21 publications

Material utilization of green waste: a review on potential valorization methods

Material utilization of green waste: a review on potential valorization methods

Refining high-quality leaf protein and valuable co-products from green biomass of Jerusalem artichoke (Helianthus tuberosus L.) for sustainable protein supply

Effects of Harvest and Fertilization Frequency on Protein Yield and Extractability From Flood-Tolerant Perennial Grasses Cultivated on a fen Peatland

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