Enhancing Precision of the Single-antigen Bead (SAB) Assay: Considerations and Challenges

Mishra, Vikash Chandra; Raina, Vimarsh

doi:10.14218/jctp.2023.00045

Cited by 1 publication

(5 citation statements)

References 49 publications

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“…Diluting the serum can fix this by removing what is blocking the antibodies. Then, the reporter antibody can perform its job properly, attaching to the antigen-antibody complex on the beads [30].…”

Section: Virtual Crossmatch (Vxm)mentioning

confidence: 99%

“…Lab variability stems from differences in SAB products and internal factors like personnel and equipment. Considering these factors is crucial for accurate SAB result interpretation [28,30].…”

Section: Virtual Crossmatch (Vxm)mentioning

confidence: 99%

“…Table 4. Representation of the key differences between cell-based cross match and virtual crossmatch tests [16][17][18][19][20][21][29][30][31].…”

Section: Hla Source Analysis Interpretationmentioning

confidence: 99%

“…This is important for organ and tissue transplants, because eplet mismatches can affect the success of the procedure. By evaluat-ing these mismatches, the tool helps to optimize donor selection, potentially improving transplant outcomes [30,[50][51][52].…”

Section: Epitope-based Matching Algorithmsmentioning

confidence: 99%

“…This tool facilitates in-depth analyses of the compatibility between donors and patients, leveraging detailed high-resolution HLA typing information. It is imperative to recognize and incorporate this synergy when selecting a potential donor [30].…”

Section: Epitope-based Matching Algorithmsmentioning

confidence: 99%

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Histocompatibility Testing: A Fundamental Aspect of Renal Transplant Workup

Mishra,

Chandra,

Raina

2024

Transplantology

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Histocompatibility testing is pivotal in any renal transplantation workup, aimed at enhancing prospective donor recipient compatibility and improving transplant outcomes. The evolution and advancement of histocompatibility testing, particularly HLA typing, have significantly improved its precision. This study outlines the historical progression from serologic to DNA-based HLA typing, emphasizing the role of HLA proteins in immune response. Anti-HLA antibodies, targeting HLA proteins, pose challenges in renal transplantation. Monitoring and managing these antibodies are critical for renal transplant success. Complement-dependent cytotoxicity crossmatch and flow cytometry crossmatch are essential techniques for assessing donor–recipient compatibility. Panel-reactive antibody assesses antibodies against a panel of donor antigens, often HLA. Higher PRA levels (percentage) complicate donor matching, requiring specialized protocols. Virtual crossmatch evaluates recipient anti-HLA antibodies against potential donors through synthetic beads. This approach predicts crossmatch outcomes by comparing antibody profiles, offering a valuable tool for the risk assessment of renal transplantation. Despite advancements, a comprehensive understanding of alloreactive immune responses requires a combination of assays, emphasizing the importance of a multifaceted approach in histocompatibility testing. This is an attempt to compile the relevant information, providing a basis for comparison in a clear and foundational format for histocompatibility testing laboratories.

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Section: Virtual Crossmatch (Vxm)mentioning

confidence: 99%

Section: Virtual Crossmatch (Vxm)mentioning

confidence: 99%

“…Table 4. Representation of the key differences between cell-based cross match and virtual crossmatch tests [16][17][18][19][20][21][29][30][31].…”

Section: Hla Source Analysis Interpretationmentioning

confidence: 99%