Enhancing plasma membrane NADPH oxidase activity increases current output by diatoms in biophotovoltaic devices

Laohavisit, Anuphon; Anderson, Alexander; Bombelli, Paolo; Jacobs, Matthew; Howe, Christopher J.; Davies, Julia M.; Smith, Alison G.

doi:10.1016/j.algal.2015.08.009

Cited by 30 publications

(31 citation statements)

References 37 publications

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“…Oxidized LRAC may be re-reduced biologically via cell surface-associated reductase enzymes (Rose, 2012;2016). Eukaryotic phytoplankton appear to utilize a transmembrane NADPH oxidase (NOX) to generate extracellular O 2 2 (Kim et al, 2000;Herv e et al, 2006;Laohavisit et al, 2015), whereas prokaryotes can employ a surface-associated animal haem peroxidase (AHP) for this function (Andeer et al, 2015) ( Fig. Finally, O 2 2 can be generated biologically by microbes, at rates that approach photochemical production measurements (Micinski et al, 1993;Petasne and Zika, 1997;Rose et al, 2008;Hansard et al, 2010;Shaked et al, 2010;Roe et al, 2016).…”

Section: Other Species May Need Cross-protection From Hoohmentioning

confidence: 99%

Cross‐protection from hydrogen peroxide by helper microbes: the impacts on the cyanobacterium Prochlorococcus and other beneficiaries in marine communities

Zinser

2018

Environ Microbiol Rep

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Hydrogen peroxide (HOOH) is a reactive oxygen species, derived from molecular oxygen, that is capable of damaging microbial cells. Surprisingly, the HOOH defence systems of some aerobes in the oxygenated marine environments are critically depleted, relative to model aerobes. For instance, the gene encoding catalase is absent in the numerically dominant photosynthetic cyanobacterium, Prochlorococcus. Accordingly, Prochlorococcus is highly susceptible to HOOH when exposed as pure cultures. Pure cultures do not exist in the marine environment, however. Catalase-positive community members can remove HOOH from the seawater medium, thus lowering the threat to Prochlorococcus and any other member that likewise lacks their own catalase. This cross-protection may constitute a loosely defined symbiosis, whereby the catalase-positive helper cells may benefit through the acquisition of nutrients released by the beneficiaries such as Prochlorococcus. Other members of the community that may be helped by the catalase-positive cells may include some lineages of Synechococcus - the sister genus of Prochlorococcus - as well as some lineages of SAR11 and ammonia oxidizing archaea and bacteria. The co-occurrence of catalase-positive and -negative members suggests that cross-protection from HOOH-mediated oxidative stress may play an important role in the construction of the marine microbial community.

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Section: Other Species May Need Cross-protection From Hoohmentioning

confidence: 99%

Cross‐protection from hydrogen peroxide by helper microbes: the impacts on the cyanobacterium Prochlorococcus and other beneficiaries in marine communities

Zinser

2018

Environ Microbiol Rep

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“…Some studies have used eukaryotic algae, predominantly either green algae, notably various species of Chlamydomonas, [26][27][28][29][30] Chlorella [31][32][33][34] and Dunaliella, [35] or diatoms, such as Phaeodactylum tricornutum and Thalassiosira pseudonana. [28] Green algae and diatoms are only very distantly related in evolutionary terms. [36] Although cells of the eukaryotic green alga Ostreococcus tauri -possibly the smallest eukaryote known -are only around 1 μm in diameter, [37] eukaryotic algal cells are typically larger than those of cyanobacteria.…”

Section: Biological Materialsmentioning

confidence: 99%

“…These are most commonly potassium ferricyanide, [10,15,16,20,26,28,29,31,58,81] and quinones such as 2,6-dichloro-p-benzoquinone (DCBQ), [14,30] 2-hydroxy-1,4-naphthoquinone (HNQ) [82][83][84][85] and 1,4-benzoquinone [24,86] (Figure 4). The major limitation of using such exogenous mediators for practical applications is that they may be toxic to the cells and expensive to scale up.…”

Section: Exogenous Eet Mechanismsmentioning

confidence: 99%

The Development of Biophotovoltaic Systems for Power Generation and Biological Analysis

et al. 2019

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Biophotovoltaic systems (BPVs) resemble microbial fuel cells, but utilise oxygenic photosynthetic microorganisms associated with an anode to generate an extracellular electrical current, which is stimulated by illumination. Study and exploitation of BPVs have come a long way over the last few decades, having benefited from several generations of electrode development and improvements in wiring schemes. Power densities of up to 0.5 W m À 2 and the powering of small electrical devices such as a digital clock have been reported. Improvements in standardisation have meant that this biophotoelectrochemical phenomenon can be further exploited to address biological questions relating to the organisms. Here, we aim to provide both biologists and electrochemists with a review of the progress of BPV development with a focus on biological materials, electrode design and interfacial wiring considerations, and propose steps for driving the field forward.[a] L.

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“…The BPV device with suspension algal cultures was prepared by loading 3 mL of the Chlorella UMACC 313 culture (at exponential phase; OD 620nm = 2.0) onto the anode through the open inlet in the middle of the algal BPV device ( Figure 2) and was then sealed with "Smith & Nephew clear, sterile waterproof film dressing." 7,37 The cathode and the anode were separated by a Perspex piece. 7,37 The cathode and the anode were separated by a Perspex piece.…”

Section: Algal Bpv Devices and Experimental Set-upmentioning

confidence: 99%

“…For the immobilized algae BPV device, the anode had the algal-alginate film (prepared as described in Section 2.2) attached to its surface ( Figure 1) and was sealed with polydimethylsiloxane (PDMS) before 2 mL Bold's Basal Medium was loaded to the device. 5,6,37 Crocodile clips and copper wires were used to connect the anode and cathode to the external circuit. 5,6,37 Crocodile clips and copper wires were used to connect the anode and cathode to the external circuit.…”

Section: Algal Bpv Devices and Experimental Set-upmentioning

confidence: 99%

Effect of different irradiance levels on bioelectricity generation from algal biophotovoltaic (BPV) devices

Thong

Phang

et al. 2019

Energy Science & Engineering

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Rapid population and economic growth in the world have accelerated the search for new sustainable and environment‐friendly energy sources. Power‐producing systems generally add to the carbon load in the environment, contributing to global climate change. In photosynthesis, energy from light splits water molecules into oxygen, protons, and electrons. Algal biophotovoltaic (BPV) platforms were developed to harvest these electrons to generate bioelectricity through algal photosynthesis. Irradiance is one of the most important parameters that determine power output efficiency from algal BPV devices. In this study, the effective range of irradiance levels for power generation from algal BPV devices comprising of suspension and alginate‐immobilized Chlorella cultures on ITO anodes was determined. Immobilized cultures were prepared by entrapping the algal cells in 2% sodium alginate solution. The algal BPV devices were illuminated by four different irradiance levels (30, 90, 150, and 210 µmol photons m−2 s−1). The maximum power density of 0.456 mW m−2 was generated from the prototype algal fuel cell at the irradiance level of 150 µmol photons m−2 s−1. At 210 µmol photons m−2 s−1, low power density was produced due to photoinhibition as indicated by Fv/Fm values generated through PAM fluorometry. In terms of carbon fixation rate, the highest value was recorded in immobilized culture at 217.11 mg CO2 L−1 d−1. The algal biophotovoltaic device is multifunctional and can provide sustainable energy with simultaneous carbon dioxide removal.

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Enhancing plasma membrane NADPH oxidase activity increases current output by diatoms in biophotovoltaic devices

Cited by 30 publications

References 37 publications

Cross‐protection from hydrogen peroxide by helper microbes: the impacts on the cyanobacterium Prochlorococcus and other beneficiaries in marine communities

Cross‐protection from hydrogen peroxide by helper microbes: the impacts on the cyanobacterium Prochlorococcus and other beneficiaries in marine communities

The Development of Biophotovoltaic Systems for Power Generation and Biological Analysis

Effect of different irradiance levels on bioelectricity generation from algal biophotovoltaic (BPV) devices

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