Enhancing plant immunity by expression of pathogen-targeted CRISPR-Cas9 in plants

Lee, Hong Gil; Kim, Duk Hyoung; Choi, Yee-Ram; Yu, Jong Pil; Hong, Sung-Ah; Seo, Pil Joon; Bae, Sangsu

doi:10.1016/j.ggedit.2021.100001

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Genome editing tools are effectively employed for in planta intervention, managing the growth of pathogen infection in the plants. Primarily validated against DNA and RNA viruses (Beet curly top virus, Tomato yellow leaf curl virus, Cucumber mosaic virus) through CRISPR/Cas protein expression ( Cao et al, 2020 ; Varanda et al, 2021 ), this strategy has now been extended to disrupt bacterial genomes using CRISPR nucleases, confirming a new resistance process in plants ( Lee et al, 2021 ).…”

Section: Genome Editing Approaches For Disease Resistancementioning

confidence: 99%

The potential of genome editing to create novel alleles of resistance genes in rice

Singh,

Devanna,

Dubey

et al. 2024

Front. Genome Ed.

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Rice, a staple food for a significant portion of the global population, faces persistent threats from various pathogens and pests, necessitating the development of resilient crop varieties. Deployment of resistance genes in rice is the best practice to manage diseases and reduce environmental damage by reducing the application of agro-chemicals. Genome editing technologies, such as CRISPR-Cas, have revolutionized the field of molecular biology, offering precise and efficient tools for targeted modifications within the rice genome. This study delves into the application of these tools to engineer novel alleles of resistance genes in rice, aiming to enhance the plant’s innate ability to combat evolving threats. By harnessing the power of genome editing, researchers can introduce tailored genetic modifications that bolster the plant’s defense mechanisms without compromising its essential characteristics. In this study, we synthesize recent advancements in genome editing methodologies applicable to rice and discuss the ethical considerations and regulatory frameworks surrounding the creation of genetically modified crops. Additionally, it explores potential challenges and future prospects for deploying edited rice varieties in agricultural landscapes. In summary, this study highlights the promise of genome editing in reshaping the genetic landscape of rice to confront emerging challenges, contributing to global food security and sustainable agriculture practices.

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Section: Genome Editing Approaches For Disease Resistancementioning

confidence: 99%

The potential of genome editing to create novel alleles of resistance genes in rice

Singh,

Devanna,

Dubey

et al. 2024

Front. Genome Ed.

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Modern Tools of Genome Engineering and Their Applications

Kaur,

Singh,

Singh

et al. 2023

Role of Microbes in Sustainable Development

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Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens

et al. 2022

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In the present scenario of a looming food crisis, improving per hectare rice productivity at a greater pace is among the topmost priorities of scientists and breeders. In the past decades, conventional, mutational, and marker-assisted breeding techniques have played a significant role in developing multiple desired rice varieties. However, due to certain limitations, these techniques cannot furnish the projected food security of the 2050 population’s aching stomachs. One of the possible options would be precise crop genome editing using various tools, viz., TALENs and CRISPR/Cas9 to resolve this multifaceted crisis. Initially, the potentiality of these technologies was tested only in the rice protoplasts. Later, the techniques were employed to edit calli with help of modified vectors, CRISPR variants, cassette cloning systems, and delivery methods. With the continuous technological advancements such as base editing, multiplexing, etc., the precision, rapidness, efficiency, reliability, potency, and range of applications of these platforms have increased and even been used for gene function studies. This leads to a revolution in the field of the rice improvement program, especially the stress tolerance against various pests and pathogens in which the susceptibility factors located within the rice genome are targeted through genome editing tools. Therefore, in this current article, we have summarized the advancements in the rice genome editing tools during the last decade concerning enhanced biotic stress tolerance. Additionally, we have focused on the regulatory aspects of genome editing with associated risks and limitations, and the prospects to reshape the rice genome for durable resistance to complex biotic stress.

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Enhancing plant immunity by expression of pathogen-targeted CRISPR-Cas9 in plants

Cited by 3 publications

References 19 publications

The potential of genome editing to create novel alleles of resistance genes in rice

The potential of genome editing to create novel alleles of resistance genes in rice

Modern Tools of Genome Engineering and Their Applications

Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens

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