Enhancing Photosynthetic Starch Production by γ-Aminobutyric Acid Addition in a Marine Green Microalga <i>Tetraselmis subcordiformis</i> under Nitrogen Stress

Ran, Wenyi; Qi, Xiao Liang; Pan, Yunyun; Xie, Tian; Zhang, Yongkui; Yao, Changhong

doi:10.1021/acs.iecr.0c00398

Cited by 16 publications

(13 citation statements)

References 49 publications

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“…Recent investigations from the author’s laboratory have also revealed GABA-induced accumulation of soluble sugars in drought-stressed snap bean leaves ( Abd El-Gawad et al, 2020 ). GABA-induced abatement of nitrogen stress in green microalga Tetraselmis sub cordiformis has been known to be associated with increased starch accumulation ( Ran et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

et al. 2021

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Despite the role of γ-aminobutyric acid (GABA) in plant tolerance to chilling stress having been widely discussed in the seedling stage, very little information is clear regarding its implication in chilling tolerance during the reproductive stage of the plant. Here, we investigated the influence of GABA (1 and 2mM) as a foliar application on tomato plants (Solanum lycopersicum L. cv. Super Marmande) subjected to chilling stress (5°C for 6h/day) for 5 successive days during the flowering stage. The results indicated that applied GABA differentially influenced leaf pigment composition by decreasing the chlorophyll a/b ratio and increasing the anthocyanin relative to total chlorophyll. However, carotenoids were not affected in both GABA-treated and non-treated stressed plants. Root tissues significantly exhibited an increase in thermo-tolerance in GABA-treated plants. Furthermore, applied GABA substantially alleviated the chilling-induced oxidative damage by protecting cell membrane integrity and reducing malondialdehyde (MDA) and H2O2. This positive effect of GABA was associated with enhancing the activity of phenylalanine ammonia-lyase (PAL), catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX). Conversely, a downregulation of peroxidase (POX) and polyphenol oxidase (PPO) was observed under chilling stress which indicates its relevance in phenol metabolism. Interesting correlations were obtained between GABA-induced upregulation of sugar metabolism coinciding with altering secondary metabolism, activities of antioxidant enzymes, and maintaining the integrity of plastids’ ultrastructure Eventually, applied GABA especially at 2mM improved the fruit yield and could be recommended to mitigate the damage of chilling stress in tomato plants.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

et al. 2021

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“…nitrogen starvation), microalgae can accumulate remarkable starch content, up to 40-45% of dry weight. [1][2][3][4] Microalgal starch could be potentially used for several industrial applications, such as bioplastics, 5 food ingredients and carrier materials. 6 However, to date, there is little information about the properties of microalgal starch.…”

Section: Introductionmentioning

confidence: 99%

Extraction of microalgal starch and pigments by using different cell disruption methods and aqueous two‐phase system

Caprio

Chelucci

Francolini

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: Microalgae can synthesize starch with productivity higher than conventional terrestrial crops, without the need for arable land. However, little is known about processes to extract starch from microalgae. Here, a biorefinery process is described including microalgal cell disruption followed by extraction of starch and pigments with aqueous two-phase system (ATPS) using choline chloride and polypropylene glycol 400. Sonication and bead milling were compared for cell disruption rate and starch extraction efficiency.RESULTS: A first order kinetic model described well the cell disruption for both the methods, with a rate 2.6 times higher for bead milling than sonication. By applying ATPS on samples with comparable cell disruption (>93%), starch was separated better after sonication (67% recovery in the pellet) than after bead milling, for which it remained equally distributed between pellet (40%) and choline chloride phase. Pigments were extracted with 42-66% yield irrespective of the cell disruption method. Microalgal starch granules had a normal and narrow distribution for size (0.93 ± 0.14 ∼m) and a gelatinization temperature between 45-55 °C.CONCLUSION: For the same cell disruption yield, different starch separation efficiencies can be achieved, depending on the cell disruption method applied. Although bead milling was faster than sonication in disrupting cells, it gave worst starch separation efficiency. The properties of the extracted microalgal starch indicate potential technical advantages, with respect to conventional starch sources, for applications in the bioplastic and food sector.

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“…In mixotrophic environments, an additional source of energy is provided in the form of an external carbon substrate (easily degradable organic compounds). However, this additional energy source inhibits photosynthesis, which stimulates the formation of pigments in microalgae such as P. subcordiformis [42].…”

Section: Discussionmentioning

confidence: 99%

Applicability of water from the Bay of Gdańsk as a growth medium for mixotrophic culture of Platymonas subcordiformis

Dudek¹,

Dębowski²,

Nowicka³

et al. 2022

Front. Biosci. (Elite Ed)

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P. subcordiformis is a potentially promising species with commercial, environmental and technological viability for industrial applications. The great potential of these microalgae lies in their fast biomass growth, pollution resistance, and compatibility with different culture media. This study aimed to determine the efficiency of P. subcordiformis biomass production in a medium prepared with water from the Bay of Gdańsk. The tested medium supported high biomass growth rates which reached 317.58 ± 42.31 mg V S /dm 3 •d in the best-performing variant, with a final concentration of 3493.3 ± 465.4 mg V S /dm 3 •d. In the autotrophic culture, nitrogen and phosphorus removal exceeded 98%. Amending the mixotrophic culture with glucose did not affect P. subcordiformis concentrations. However, it did significantly limit the demand for nutrients in the biomass and reduced chlorophyll a production in the cells.

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Enhancing Photosynthetic Starch Production by γ-Aminobutyric Acid Addition in a Marine Green Microalga Tetraselmis subcordiformis under Nitrogen Stress

Cited by 16 publications

References 49 publications

Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

Protective Effect of γ-Aminobutyric Acid Against Chilling Stress During Reproductive Stage in Tomato Plants Through Modulation of Sugar Metabolism, Chloroplast Integrity, and Antioxidative Defense Systems

Extraction of microalgal starch and pigments by using different cell disruption methods and aqueous two‐phase system

Applicability of water from the Bay of Gdańsk as a growth medium for mixotrophic culture of Platymonas subcordiformis

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