Enhancing photosynthesis at high light levels by adaptive laboratory evolution

Dann, Marcel; Ortiz, Edgardo M.; Moritz, Thomas; Guljamow, Arthur; Lehmann, Martin; Schaefer, Hanno; Leister, Dario

doi:10.1038/s41477-021-00904-2

Cited by 25 publications

(18 citation statements)

References 69 publications

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“…Reduced absorption cross section of PSII resulting from antenna truncation leads to light limitation and reduced growth compared to the WT under typical growth conditions 18–20 , making it difficult to directly compare the physiology and light response of these strains in bulk culture. Recently, adaptive laboratory evolution studies under high-light conditions selected for strains with reduced levels of antenna 21 . These studies provided the motivation to directly compare growth and photoinhibition of WT and Δ cpc strains in 2D cultures.…”

Section: Resultsmentioning

confidence: 99%

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Asymmetric survival in single-cell lineages of cyanobacteria in response to photodamage

Tay

Cameron

2022

Preprint

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Oxygenic photosynthesis is driven by the coupled action of the light-dependent pigment protein complexes, photosystem I and II, located within the internal thylakoid membrane system. However, photosystem II is known to be prone to photooxidative damage. Thus, photosynthetic organisms have evolved a repair cycle to continuously replace the damaged proteins in photosystem II. However, it has remained difficult to deconvolute the damage and repair processes using traditional ensemble approaches. Here we demonstrate an automated approach using time-lapse fluorescence microscopy and computational image analysis to study the dynamics and effects of photodamage in single cells at sub-cellular resolution in cyanobacteria. By growing cells in a two-dimensional layer, we avoid shading effects, thereby generating uniform and reproducible growth conditions. Using this platform, we analyzed the growth and physiology of multiple strains simultaneously under defined photoinhibitory conditions stimulated by UV-A light. Our results reveal an asymmetric cellular response to photodamage between sibling cells and the generation of an elusive subcellular structure, here named a photoendosome, derived from the thylakoid which could indicate the presence of a previously unknown photoprotective mechanism. We anticipate these results to be a starting point for further studies to better understand photodamage and repair at the single-cell level.

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Section: Resultsmentioning

confidence: 99%

“…Recently, adaptive laboratory evolution studies under high-light conditions selected for strains with reduced levels of antenna 21 . These studies provided the motivation to directly compare growth and photoinhibition of WT and ∆cpc strains in 2D cultures.…”

Section: Experimental Descriptionmentioning

confidence: 99%

Asymmetric survival in single-cell lineages of cyanobacteria in response to photodamage

Tay

Cameron

2022

Preprint

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“…Hydrophobic pigments were extracted and quantified as previously described [62,63]. Phycobiliprotein contents were estimated based on whole-cell UV/Vis absorbance spectra obtained at seven days past inoculation using a NanoDrop™ 2000c spectrophotometer (ThermoFisher Scientific Inc., Waltham, MA, USA) as previously described [64,65].…”

Section: Synechocystis Pigment Extraction and Quantificationmentioning

confidence: 99%

Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

Minagawa

Dann

2023

Plants

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Cyanobacteria mostly rely on the active uptake of hydrated CO2 (i.e., bicarbonate ions) from the surrounding media to fuel their inorganic carbon assimilation. The dehydration of bicarbonate in close vicinity of RuBisCO is achieved through the activity of carboxysomal carbonic anhydrase (CA) enzymes. Simultaneously, many cyanobacterial genomes encode extracellular α- and β-class CAs (EcaA, EcaB) whose exact physiological role remains largely unknown. To date, the CahB1 enzyme of Sodalinema gerasimenkoae (formerly Microcoleus/Coleofasciculus chthonoplastes) remains the sole described active extracellular β-CA in cyanobacteria, but its molecular features strongly suggest it to be a carboxysomal rather than a secreted protein. Upon expression of CahB1 in Synechocystis sp. PCC6803, we found that its expression complemented the loss of endogenous CcaA. Moreover, CahB1 was found to localize to a carboxysome-harboring and CA-active cell fraction. Our data suggest that CahB1 retains all crucial properties of a cellular carboxysomal CA and that the secretion mechanism and/or the machinations of the Sodalinema gerasimenkoae carboxysome are different from those of Synechocystis.

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“…High-light- and high-temperature-tolerances are especially important for outdoor applications. Adaptive laboratory evolution (ALE) in combination with systems biology approaches, were successfully applied for Chlamydomonas, Synechocystis , and Synechococcus ( Zhang et al, 2021b ; Srivastava et al, 2021 ; LaPanse et al, 2021 ; Dann et al, 2021 ; Mukherjee et al, 2020 ). Synechocystis sp.…”

Section: Pathway Engineeringmentioning

confidence: 99%

“…Synechocystis sp. PCC 6803, for instance, was adapted to cope with additional (light) energy on account of a higher light harvesting capacity ( Dann et al, 2021 ), auguring well for an effective use of high light intensities. Relatively low growth rates constitute another obstacle for photo-biotechnology ( Table 3 ).…”

Section: Pathway Engineeringmentioning

confidence: 99%

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

Theodosiou

Tüllinghoff

Toepel

et al. 2022

Front. Bioeng. Biotechnol.

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The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes.

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Enhancing photosynthesis at high light levels by adaptive laboratory evolution

Cited by 25 publications

References 69 publications

Asymmetric survival in single-cell lineages of cyanobacteria in response to photodamage

Asymmetric survival in single-cell lineages of cyanobacteria in response to photodamage

Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis

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