Enhancing online protein isolation as intact species from soy flour samples by actively modulated two-dimensional liquid chromatography (2D-LC)

Nardiello, Donatella; Melfi, Maria Teresa; Pignatelli, Carla; Centonze, Diego

doi:10.1016/j.jpba.2019.112976

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…The third step (second Dimension separation): LV position 10/1; RV position 10/1. Reprinted with permission, [51].…”

Section: Size‐exclusion Chromatographymentioning

confidence: 99%

“…Although not used for routine bio-purification studies like offline approaches, the online approaches are more interested in the proof of concept of new analytical instrumentation. Nardielloťs group employed UV detection to monitor the orthogonal SEC-RP separation of proteins from eggs (in the first work) and soy flour (in the second work) [50,51]. The online setup consisted of multiport switching valves connected with two analytical pumps, and one RP trapping column between 1 D and 2 D to reduce dead volumes and band-broadening effects.…”

Section: Online Sec Approachesmentioning

confidence: 99%

“…Then, after a 2-minute wash of the trapping column with water + 0.1% TFA mobile phase, the flow was delivered in a back-flush to release the trapped proteins to the analytical column where they were separated by gradient elution RPLC. With the aid of an Automatic Fraction Collector at the end of the RP column, these two reported systems consisted of automated methods to purify proteins on food samples based on the SEC-RP separation to be further characterized by MS [50,51].…”

Section: Online Sec Approachesmentioning

confidence: 99%

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Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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In this article, we review stationary phase‐assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low‐pH reversed‐phase liquid chromatography‐tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high‐pH reversed‐phase liquid chromatography > ion exchange chromatography.

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“…The third step (second Dimension separation): LV position 10/1; RV position 10/1. Reprinted with permission, [51].…”

Section: Size‐exclusion Chromatographymentioning

confidence: 99%

Section: Online Sec Approachesmentioning

confidence: 99%

Section: Online Sec Approachesmentioning

confidence: 99%

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Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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“…The advantages of this method are not only to avoid time-consuming and tedious purification steps prior to the analysis, but also due to the influence of light, heat, and other factors, the geometric isomerization of astaxanthin during the separation process is greatly reduced. However, the solvent strength or viscosity mismatch between the two dimensions may lead to losses of separation power in the second dimension separation and cause peak distortion and broadening [40]. In general, the newly proposed 2-D-LC method facilitates the monitoring of astaxanthin isomerization during extraction and processing of valueadded products containing astaxanthin from P. rhodozyma and other organisms.…”

Section: Real Sample Applicationmentioning

confidence: 99%

Two‐dimensional liquid chromatography analysis of all‐trans‐, 9‐cis‐, and 13‐cis‐astaxanthin in raw extracts from Phaffia rhodozyma

Wang

et al. 2020

J of Separation Science

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An effective two‐dimensional liquid chromatography method has been established for the analysis of all‐trans‐astaxanthin and its geometric isomers from Phaffia rhodozyma employing a C18 column at the first dimension and a C30 column in the second dimension, connected by a 10‐port valve using the photo‐diode array detector. The regression equation of astaxanthin calibration curve was established, and the precision and accuracy values were found to be in the range of 0.32–1.14% and 98.21–106.13%, respectively. By using two‐dimensional liquid chromatography, it was found that day light, ultrasonic treatment, and heat treatment have significant influence on the content of all‐trans‐astaxanthin in the extract from P. rhodozyma due to the transformation of all‐trans‐astaxanthin to cis‐astaxanthin. The day light and ultrasonic treatments more likely transform all‐trans‐astaxanthin to 9‐cis‐astaxanthin, and the thermal treatment transforms all‐trans‐astaxanthin to 13‐cis‐astaxanthin. These results indicate that the two‐dimensional liquid chromatography method can facilitate monitoring astaxanthin isomerization in the raw extract from P. rhodozyma. In addition, the study will provide a general reference for monitoring other medicals and bioactive chemicals with geometric isomers.

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“…Zhu , has developed a trapping array-based preparative 2D-LC system for the isolating of herbal medicines. And few preparative 2D-LC configurations run in heart-cut mode, with one − or two fractions ,− were transferred to 2 D separations. Although preparative 2D-LC methods have been established and are quite powerful, exhaustive separations of very complex samples are still challenging. , This has motivated researchers to investigate the potential benefits of adding more LC columns to a 2D-LC system .…”

Section: Introductionmentioning

confidence: 99%

Less Configuration and More Dimensionality: Preparative Heart-Cut Multidimensional Liquid Chromatography Based on Trapping Arrays

Zhai

Peng

et al. 2022

Anal. Chem.

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The resolving power of multiple dimensional liquid chromatography (mD-LC) is multiplicative as it adds dimensions. However, the issue in creating a preparative mD-LC system is that the higher the dimensionality, the more complicated the system configuration. Thus, we presented a new configuration of preparative mD-LC using one set of LC modules and trapping array-based multiple heart-cut interfaces. A preparative two-dimensional liquid chromatography (2D-LC) separation of herbal medicine formulation produced 40 compounds with a purity of >90%. During the separation process, the interface stores the fractions and allocates positions for the fractions from a different dimension; LC draws the fraction from the interface, makes nD separation, and sends isolated fractions to the interface. By repeating this process, we achieved variable dimensionality of LC separations. We also presented a preparative 3D-LC separation of herbal medicines to validate the principle of “less configuration and more dimensionality”. Thus, we can explore the higher dimensional preparative separations. The developed preparative mD-LC displayed exceptional power in the isolation of various compounds and has great potential in the application of natural products.

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Enhancing online protein isolation as intact species from soy flour samples by actively modulated two-dimensional liquid chromatography (2D-LC)

Cited by 8 publications

References 29 publications

Recent stationary phase‐based fractionation strategies in proteomic analysis

Recent stationary phase‐based fractionation strategies in proteomic analysis

Two‐dimensional liquid chromatography analysis of all‐trans‐, 9‐cis‐, and 13‐cis‐astaxanthin in raw extracts from Phaffia rhodozyma

Less Configuration and More Dimensionality: Preparative Heart-Cut Multidimensional Liquid Chromatography Based on Trapping Arrays

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