Enhancing methane production in anaerobic digestion through hydrogen assisted pathways – A state-of-the-art review

D’Silva, Tinku Casper; Isha, Adya; Chandra, Ram; Vijay, Virendra Kumar; Subbarao, P.M.V.; Kumar, Ritunesh; Chaudhary, Ved Prakash; Singh, Harjit; Khan, Abid Ali; Tyagi, Vinay Kumar; Kovács, Kornél L.

doi:10.1016/j.rser.2021.111536

Cited by 65 publications

(20 citation statements)

References 166 publications

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“…However, 22% yield of CH 4 was observed at 45°C. These CH 4 production levels are slightly lower than those reported in previous studies owing to the influence of different feedstock characteristics, feeding rates, incubation periods, and thermal conditions [40,[43][44][45].…”

Section: Reactor Performance In Biogas Productioncontrasting

confidence: 70%

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

Sudiartha

Imai

2022

J. of Wat. & Envir. Tech.

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Operating temperature significantly affects biogas output, process stability, and microbial communities involved in anaerobic digestion. There are several unanswered questions regarding how microbial communities adapt in correlation with biogas production performance, especially when a digester fails to maintain thermophilic conditions. In this study, long-term lab-scale anaerobic digestion was carried out using two fed-batch reactors at 55°C, with subsequent decreases in temperature to 48°C and 45°C. Within the first month of incubation, methane (CH 4 ) production increased by approximately 11.18% following a reduction in temperature from 55°C to 48°C. However, the methane production decreased by 33% after the temperature was downshifted to 45°C. Despite the difference in methane production, the thermophilic methanogen population in both reactors declined significantly in the first month with a temperature decrease. After two months of incubation, these methanogenic communities recovered faster at 48°C than at 45°C, which was highlighted by the rapid colonization of Methanosaeta, Methanobacterium, and Methanothermobacter. Notably, Methanosaeta was the most abundant methanogen under all temperature conditions, indicating its thermotolerance.

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Section: Reactor Performance In Biogas Productioncontrasting

confidence: 70%

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

Sudiartha

Imai

2022

J. of Wat. & Envir. Tech.

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“…Chloroflexi plays an important role in AD and can degrade both monosaccharides and polysaccharides, as well as generate acetic acid . Some of the Actinobacteria contribute to VFAs and propionate production along with hydrolysis Thermotogata, which is commonly found in thermophilic temperature reactors, is capable of degrading acetic acid .…”

Section: Resultsmentioning

confidence: 99%

Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

et al. 2023

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Temperature plays an important role in anaerobic digestion (AD), and different substrates have different optimum temperatures in AD. However, the effect of temperature on the performance of AD when cellulosic ethanol wastewater was used as a substrate was rarely reported. Therefore, the digestion characteristics of cellulosic ethanol wastewater at 25, 35, 45, and 55 °C were investigated, and the microbial communities of the sludge sample were analyzed after fermentation. The results showed that the cumulative methane production was the highest at 55 °C, 906.40 ± 50.67 mL/g VS, which was 81.06, 72.42, and 13.33% higher than that at 25, 35, and 45 °C, respectively. The content of methane was 68.13, 49.26, 70.46, and 85.84% at the terminal period of fermentation at temperatures of 25, 35, 45, and 55 °C, respectively. The testing of volatile fatty acids (VFAs) indicated that the accumulation of VFAs did not occur when the fermentation was carried out at 25, 35, and 45 °C; however, the VFA content at 55 °C was much larger than that in the three groups (25, 35, and 45 °C), and the ratio of propionic acid to acetic acid was larger than 1.4 at the late stage of fermentation, so it inhibited the fermentation. The diversity of the microbial community indicated that the floral structure and metabolic pathway of fermentation were alike at 25 and 35 °C. Firmicutes and Proteobacteria were the main flora covering the 25–55 °C-based phylum or below it. The relative abundance of Methanosaeta was the highest when fermentation temperatures were 25 and 35 °C; however, its relative abundance decreased sharply and the relative abundance of Methanosarcina increased substantially when the temperature increased from 35 to 45 °C, which indicated that Methanosarcina can exist in higher temperatures. At the same time, hydrogenotrophic methanogens such as Methanoculleus and Methanothermobacter were dominant when fermentation temperatures were 45 and 55 °C, which indicated that the metabolic pathway changed from acetoclastic methanogenesis to hydrogenotrophic methanogenesis.

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“…The methanogenic genus, Methanosarcina, apparently did not suffer much from starvation stress (Table 1) and maintained its predominance in the AD community [59,72,73]. The abundance of the genus Methanothermobacter was also largely unaffected by the starvation stress.…”

Section: Discussionmentioning

confidence: 98%

Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

et al. 2021

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The performance of a mixed microbial community was tested in lab-scale power-to-methane reactors at 55 °C. The main aim was to uncover the responses of the community to starvation and stoichiometric H2/CO2 supply as the sole substrate. Fed-batch reactors were inoculated with the fermentation effluent of a thermophilic biogas plant. Various volumes of pure H2/CO2 gas mixtures were injected into the headspace daily and the process parameters were followed. Gas volumes and composition were measured by gas-chromatography, the headspace was replaced with N2 prior to the daily H2/CO2 injection. Total DNA samples, collected at the beginning and end (day 71), were analyzed by metagenome sequencing. Low levels of H2 triggered immediate CH4 evolution utilizing CO2/HCO3− dissolved in the fermentation effluent. Biomethanation continued when H2/CO2 was supplied. On the contrary, biomethane formation was inhibited at higher initial H2 doses and concomitant acetate formation indicated homoacetogenesis. Biomethane production started upon daily delivery of stoichiometric H2/CO2. The fed-batch operational mode allowed high H2 injection and consumption rates albeit intermittent operation conditions. Methane was enriched up to 95% CH4 content and the H2 consumption rate attained a remarkable 1000 mL·L−1·d−1. The microbial community spontaneously selected the genus Methanothermobacter in the enriched cultures.

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Enhancing methane production in anaerobic digestion through hydrogen assisted pathways – A state-of-the-art review

Cited by 65 publications

References 166 publications

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

An Investigation of Temperature Downshift Influences on Anaerobic Digestion in the Treatment of Municipal Wastewater Sludge

Effect of Temperature on Anaerobic Fermentation of Poplar Ethanol Wastewater: Performance and Microbial Communities

Development of Stable Mixed Microbiota for High Yield Power to Methane Conversion

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