Enhancing maturity in 3D kidney micro‐tissues through clonogenic cell combinations and endothelial integration
Fatemeh Abdollahzadeh,
Niloofar Khoshdel‐Rad,
Khadijeh Bahrehbar
et al.
Abstract:As an advance laboratory model, three‐dimensional (3D) organoid culture has recently been recruited to study development, physiology and abnormality of kidney tissue. Micro‐tissues derived from primary renal cells are composed of 3D epithelial structures representing the main characteristics of original tissue. In this research, we presented a simple method to isolate mouse renal clonogenic mesenchymal (MLCs) and epithelial‐like cells (ELCs). Then we have done a full characterization of MLCs using flow cytomet… Show more
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