The zeta potential of nanoparticles
impacts their distribution
and metabolism in the body as well as their interaction with medications
of varying charges, hence altering therapeutic efficacy and safety.
In this paper, the external charges of liposomes were regulated by
utilizing a simple and economical method based on competition for
protons of cationic chitosan (CS) and anion hyaluronic acid (HA).
The charge regulation of a liposomal membrane is generally accomplished
by adjusting the ratio of charged lipids within a liposome (e.g.,
cationic DOTAP or anionic DOPS), the stability of which was maintained
by the coating materials of cationic chitosan (CS) or anion hyaluronic
acid (HA). A series of nanoparticles could respond to pH-stimulation
with adjustable surface charge. Moreover, the sizes of liposomes coated
with CS and HA remain within a narrow range. In vitro cytotoxicity
tests revealed that the nanocarriers were safe, and the nanoparticles
containing antitumor medicines were efficient in tumor therapy. Considering
liposomes with different external surface charges could be aimed at
diverse therapy purposes. The strategies for regulating liposomal
surface charges with high encapsulation rates and certain release
cycles reported here could provide a versatile platform as carriers
for the delivery of drugs and other macromolecules into human bodies.