Enhancing forensic efficiency of STR typing using mass spectrometry-based assay

Leonov, Sergey V.; Zemskova, E. Yu.; Timoshenko, Tatyana; Иванов, П. Л.

doi:10.1016/j.fsigss.2013.10.143

Cited by 3 publications

(7 citation statements)

References 2 publications

(3 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…затрагивающие собственно степень выраженности полиморфизма нуклеотидной последовательности в STR-локусах хромосомной ДНК человека, были развернуты лишь относительно недавно [3,4]. Между тем, как ранее показано в наших работах [4][5][6]8], именно здесь заключается ресурс для увеличения дискриминирующего потенциала STR-маркеров.…”

Section: полиморфизм нуклеотидной последовательности днк как источникunclassified

“…Продукты ПЦР фракционировали масс-спектро метричес ки с использованием автоматизированного комплекса PLEX-ID Instrument («Abbott Molecular», США) [3][4][5][6][7][8]10].…”

Section: материал и методыunclassified

“…Масс-спектрометрический анализ позволяет выявить так называемые масс-варианты амплифицированных фрагментов ДНК, представляющие собой аллельные состояния STR-локусов, различающиеся не количеством тандемных повторов (как «канонические» STR-аллели), а своей нуклеотидной последовательностью [5][6][7][8]. Эти различия проявляются в виде точковых нуклеотидных замен (SNP).…”

Section: результаты и обсуждениеunclassified

See 2 more Smart Citations

Polymorphism of DNA nucleotide sequence as a source of enhancement of the discrimination potential of the STR-markers

et al. 2016

Sud.-med. ekspert.

View full text Add to dashboard Cite

The objective of the present pilot investigation was to reveal and to study polymorphism of nucleotide sequence in the alleles of STR loci of human autosomal DNA with special reference to the role of this phenomenon as a source of the differences between homonymous allelic variants. The secondary objection was to evaluate the possibility of using the data thus obtained for the enhancement of the informative value of the forensic medical genotyping of STR loci by means of identification of single nucleotide polymorphisms (SNP) for the purpose of extending their allelic spectrum. The methodological basis of the study was constituted by the comprehensive amplified fragment length polymorphism (AFLP) analysis and amplified fragment sequence polymorphisms (AFSP) analysis of DNA with the use of the PLEX-ID^TM analytical mass-spectrometry platform (Abbot Molecular, USA). The study has demonstrated that polymorphism of DNA nucleotide sequence can be regarded as the possible source of enhancement of the discriminating potential of STR markers. It means that the analysis of polymorphism of DNA nucleotide sequence for genotyping AFLP-type markers of chromosomal DNA can considerably increase the effectiveness of their application as individualizing markers for the purpose of molecular genetic expertises.

show abstract

Section: полиморфизм нуклеотидной последовательности днк как источникunclassified

Section: материал и методыunclassified

Section: результаты и обсуждениеunclassified

See 1 more Smart Citation

Polymorphism of DNA nucleotide sequence as a source of enhancement of the discrimination potential of the STR-markers

et al. 2016

Sud.-med. ekspert.

View full text Add to dashboard Cite

show abstract

“…Методической основой работы является комплексный анализ полиморфизма длины амплифицированных фрагментов (ПДАФ) и полиморфизма последовательности амплифицированных фрагментов (ППАФ) ДНК с помощью масс-спектрометрической аналитической платформы PLEX-ID («Abbott Molecular», США) [10][11][12]. Принцип действия, особенности и опыт использования этой передовой технологии описаны ранее [2][3][4]11].…”

Section: материал и методыunclassified

“…Судебно-медицинСкая экСпертиза, 5,2016 меном различий в одноименных STR-аллелях. Этой тематике посвящен ряд наших ранее опубликованных работ [2][3][4], в которых изучали характер и уровень индивидуальных различий одноименных STR-аллелей по признаку присутствия или отсутствия в них точковых нуклеотидных замен (single nucleotide polymorphism -SNP) в аспекте возможного повышения информативности судебноэкспертного генотипирования STR-локусов за счет выявления в их аллелях SNP и расширения таким образом их аллельного спектра.…”

unclassified

The interpopulation differences between nucleotide sequence polymorphisms in alleles of the STR-loci of human chromosomal DNA

et al. 2016

Sud.-med. ekspert.

View full text Add to dashboard Cite

The objective of the present work was to study the phenomenon of nucleotide sequence polymorphism in alleles of the STR-loci of human chromosomal DNA and to estimate its interpopulation differences with a view to the search for the molecular-genetic markers to be used as an efficient tool for the determination of belonging of the subjects of interest to a given population. We undertook the comprehensive analysis of amplified DNA fragment sequence polymorphism (AFSP) and amplified DNA fragment length polymorphism (AFLP) with the use of the PLEX-ID-TM analytical mass-spectrometry platform (Abbott Molecular, USA). The interpopulation differences were estimated in terms of the presence or the absence of single nucleotide replacements (SNP) in the STR markers based on a few population samples. Some of the loci of interest were found to have allelic variants the occurrence of which was significantly different in individual samples. Such alleles are of importance for the further investigation since they can be regarded as potential ethno-geographical markers. Their application opens up the new promising prospects for the expert detection of the ethnic affiliation of individual subjects.

show abstract

On the Expansion of the Scope of Molecular Genetic Examinations and Improvement Ofmolecular Genetic Technologies

Иванов¹,

Земскова²

2015

Russian Journal of Forensic Medicine

View full text Add to dashboard Cite

show abstract

Enhancing forensic efficiency of STR typing using mass spectrometry-based assay

Cited by 3 publications

References 2 publications

Polymorphism of DNA nucleotide sequence as a source of enhancement of the discrimination potential of the STR-markers

Polymorphism of DNA nucleotide sequence as a source of enhancement of the discrimination potential of the STR-markers

The interpopulation differences between nucleotide sequence polymorphisms in alleles of the STR-loci of human chromosomal DNA

On the Expansion of the Scope of Molecular Genetic Examinations and Improvement Ofmolecular Genetic Technologies

Contact Info

Product

Resources

About