Enhancing Effects of Immobilized Chondroitin Sulfate on Odontogenic Differentiation of Dental Pulp Stem Cells and Reparative Dentin Formation

Xu, Ruoman; Zhou, Ziyu; Lin, Danle; Yuan, Linwang; Wang, Siyu; Xu, Meng; Chen, Yanan; Hu, Xiaoli

doi:10.1016/j.joen.2023.04.012

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…However, research has shown that when scaffolds containing hDPSCs alone are implanted in nude mice subcutaneously, only limited mineralized tissue can be formed (Zhang et al., 2006). Narrow root canals and foramina restrict the process of tissue infiltration and revascularization, which is also detrimental to implanted DPSCs (Xu et al., 2023). In general, there are still many limitations in the application of DPSCs in the regeneration of the dentine–pulp complex; therefore, it is necessary to apply new and optimized strategies to protect and enhance the function of DPSCs (Yang et al., 2007).…”

Section: Discussionmentioning

confidence: 99%

PDGF‐AA guides cell crosstalk between human dental pulp stem cells in vitro via the PDGFR‐α/PI3K/Akt axis

Xu,

Wei,

Liu

et al. 2024

Int Endodontic J

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AimTo explore the influence of PDGF‐AA on cell communication between human dental pulp stem cells (DPSCs) by characterizing gap junction intercellular communication (GJIC) and its potential biomechanical mechanism.MethodologyQuantitative real‐time PCR was used to measure connexin family member expression in DPSCs. Cell migration and CCK‐8 assays were utilized to examine the influence of PDGF‐AA on DPSC migration and proliferation. A scrape loading/dye transfer assay was applied to evaluate GJIC triggered by PDGF‐AA, a PI3K/Akt signalling pathway blocker (LY294002) and a PDGFR‐α blocker (AG1296). Western blotting and immunofluorescence were used to test the expression and distribution of the Cx43 and p‐Akt proteins in DPSCs. Scanning electron microscopy (SEM) and immunofluorescence were used to observe the morphology of GJIC in DPSCs.ResultsPDGF‐AA promoted gap junction formation and intercellular communication between human dental pulp stem cells. PDGF‐AA upregulates the expression of Cx43 to enhance gap junction formation and intercellular communication. PDGF‐AA binds to PDGFR‐α and activates PI3K/Akt signalling to regulate cell communication.ConclusionsThis research demonstrated that PDGF‐AA can enhance Cx43‐mediated GJIC in DPSCs via the PDGFR‐α/PI3K/Akt axis, which provides new cues for dental pulp regeneration from the perspective of intercellular communication.

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Section: Discussionmentioning

confidence: 99%

PDGF‐AA guides cell crosstalk between human dental pulp stem cells in vitro via the PDGFR‐α/PI3K/Akt axis

Xu,

Wei,

Liu

et al. 2024

Int Endodontic J

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“…The images were captured by a slice section scanner (Aperio AT2; Leica, Wetzlar, Germany), and the expression of DMP1 and ALP was quantified by ImageJ software. The area of reparative dentin was calculated by ImageJ software as previously reported. , …”

Section: Experimental Sectionmentioning

confidence: 99%

Electrical Stimulations Generated by P(VDF-TrFE) Films Enhance Adhesion Forces and Odontogenic Differentiation of Dental Pulp Stem Cells (DPSCs)

Lin,

Zhou,

Zhang

et al. 2024

ACS Appl. Mater. Interfaces

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Biophysical and biochemical cues of biomaterials can regulate cell behaviors. Dental pulp stem cells (DPSCs) in pulp tissues can differentiate to odontoblast-like cells and secrete reparative dentin to form a barrier to protect the underlying pulp tissues and enable complete pulp healing. Promotion of the odontogenic differentiation of DPSCs is essential for dentin regeneration. The effects of the surface potentials of biomaterials on the adhesion and odontogenic differentiation of DPSCs remain unclear. Here, poly(vinylidene fluoride-trifluoro ethylene) (P(VDF-TrFE)) films with different surface potentials were prepared by the spin-coating technique and the contact poling method. The cytoskeletal organization of DPSCs grown on P(VDF-TrFE) films was studied by immunofluorescence staining. Using atomic force microscopy (AFM), the lateral detachment forces of DPSCs from P(VDF-TrFE) films were quantified. The effects of electrical stimulation generated from P(VDF-TrFE) films on odontogenic differentiation of DPSCs were evaluated in vitro and in vivo. The unpolarized, positively polarized, and negatively polarized films had surface potentials of −52.9, +902.4, and −502.2 mV, respectively. DPSCs on both negatively and positively polarized P(VDF-TrFE) films had larger cell areas and length-to-width ratios than those on the unpolarized films (P < 0.05). During the detachment of DPSCs from P(VDF-TrFE) films, the average magnitudes of the maximum detachment forces were 29.4, 72.1, and 53.9 nN for unpolarized, positively polarized, and negatively polarized groups, respectively (P < 0.05). The polarized films enhanced the mineralization activities and increased the expression levels of the odontogenic-related proteins of DPSCs compared to the unpolarized films (P < 0.05). The extracellular signal-regulated kinase (ERK) signaling pathway was involved in the odontogenic differentiation of DPSCs as induced by surface charge. In vivo, the polarized P(VDF-TrFE) films enhanced adhesion of DPSCs and promoted the odontogenic differentiation of DPSCs by electrical stimulation, demonstrating a potential application of electroactive biomaterials for reparative dentin formation in direct pulp capping.

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Enhancing Effects of Immobilized Chondroitin Sulfate on Odontogenic Differentiation of Dental Pulp Stem Cells and Reparative Dentin Formation

Cited by 2 publications

References 33 publications

PDGF‐AA guides cell crosstalk between human dental pulp stem cells in vitro via the PDGFR‐α/PI3K/Akt axis

PDGF‐AA guides cell crosstalk between human dental pulp stem cells in vitro via the PDGFR‐α/PI3K/Akt axis

Electrical Stimulations Generated by P(VDF-TrFE) Films Enhance Adhesion Forces and Odontogenic Differentiation of Dental Pulp Stem Cells (DPSCs)

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