Enhancing Effect of Interferon Pretreatment on Interferon Production

Ito, F.; Kobayashi, S

doi:10.1111/j.1348-0421.1974.tb00949.x

Cited by 8 publications

(15 citation statements)

References 14 publications

(22 reference statements)

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“…It has also been reported that the maximally primed state of cells can be achieved with relatively moderate concentrations of IFN (Stewart et aL, 1971 ;Ito & Kobayashi, 1974). These authors and De Maeyer-Guignard et al (1980) found that increasing the pretreatment dose of IFN above the optimal level did not cause any further change in priming.…”

Section: Dose-response To Ifnmentioning

confidence: 64%

“…Stewart et al (1973) and Ito & Kobayashi (1974) demonstrated that priming activity co-purifies with the antiviral activity in different I FN preparations, suggesting that IFN per se exerts these seemingly unrelated effects on cells. Priming of cells is frequently used for the enhancement of IFN yields in large-scale conventional IFN-producing systems (Cantell et al, 1974;Clark & Hirtenstein, 1981 ;Edy et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

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Study of the in vivo Priming Effect of Interferon in Mice

Rosztóczy

1986

Journal of General Virology

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SUMMARYIntramuscular injection of mice with 2000 IU/g partially purified murine interferon (IFN) alpha/beta 3 h before the induction of IFN by intraperitoneally administered 3 ~tg/g poly rI :rC enhanced early IFN production. The differences between the serum IFN levels of IFN-pretreated and control animals were about 10-fold during the first 2 to 3 h of in vivo IFN production. In later stages these differences tended to decrease, and from 8 h post-induction they disappeared. IFN exerted its in vivo priming activity equally after intravenous, intraperitoneal or intramuscular injection. A similar enhancement of IFN production was observed when it was induced by poly rI :rC administered either intraperitoneally or intramuscularly. The duration of IFN pretreatment influenced the establishment of the in vivo primed state. Following administration of 2000 IU/g murine IFN alpha/beta intramuscularly, maximal priming developed after 3 h, and no primed IFN response was detected when the inoculation of IFN preceded inducer administration by 12 h or more. The manifestation of in vivo priming was optimal when 1500 to 3000 IU/g pretreating doses of IFN were applied. Reduction of the amount of injected IFN below this level markedly decreased priming, indicating a time-and dose-dependent induction of priming in vivo.

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Section: Dose-response To Ifnmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Study of the in vivo Priming Effect of Interferon in Mice

Rosztóczy

1986

Journal of General Virology

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“…We previously demonstrated that the priming activity of IF always paralleled the antiviral activity regardless of the source and purity of the IF preparation [4]. The finding suggested that these activities could be ascribed to IF molecules and that there might be some common steps in the development of the primed and antiviral states in IF-treated cells.…”

Section: Introductionmentioning

confidence: 96%

“…IF was assayed on L cells by the plaque reduction dose method (the PRD50 method), as described previously [4]. In some experiments, a method based on inhibition of viral ribonucleic acid synthesis (INAS50 method) was employed for IF titration using L-MS cells [11].…”

Section: Methodsmentioning

confidence: 99%

“…However, it is still unclear whether these activities are ascribable to IF itself or to some other components in IF preparations. It is also unknown whether IF exerts these activities on cells through a common induction mechanism.We previously demonstrated that the priming activity of IF always paralleled the antiviral activity regardless of the source and purity of the IF preparation [4]. The finding suggested that these activities could be ascribed to IF molecules and that there might be some common steps in the development of the primed and antiviral states in IF-treated cells.…”

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confidence: 96%

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Requirement of Newly Synthesized Protein for the Priming Activity of Interferon

Ito

Suzuki

Kobayashi

1975

Japanese Journal of Microbiology

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Pretreatment of mouse L cells with interferon (IF) enhanced IF production in response to polyinosinic-polycytidylic acid (poly I.poly C). Post-treatment of cells with IF caused no significant enhancement of IF production. The enhancing effect of IF pretreatment (priming) reached a maximum after incubation with IF (10 or 100 units/ml) for 4-6 hr at 37 C, but this effect was absent \A hen the incubation was done at 4 C. Cells which were incubated for additional several hours at 37 C after IF pretreatment at 4 C did not develop the primed state nor the antiviral state. The presence of protein synthesis inhibitors during the IF pretreatment depressed, though not completely, the development of the primed state. The residual priming effect was lost when the cells were incubated with the inhibitors at 37 C for 2 hr before they were exposed to poly I.poly C. There was no significant difference in the binding rate of poly I.poly C to cells between IF-treated and untreated cells. The degradation rate of cell-bound poly I.poly C and its sensitivity to exogenous pancreatic ribonucicase in the pretreated cells were also similar to those in the untreated cells.Interferon (IF) preparations have been shown to possess various activities other than the antiviral activity. These are inhibition of cell growth [3], enhancement or suppression of IF production [8,10], and enhancement of cytopathic effect of double-stranded RNA [9]. However, it is still unclear whether these activities are ascribable to IF itself or to some other components in IF preparations. It is also unknown whether IF exerts these activities on cells through a common induction mechanism.We previously demonstrated that the priming activity of IF always paralleled the antiviral activity regardless of the source and purity of the IF preparation [4]. The finding suggested that these activities could be ascribed to IF molecules and that there might be some common steps in the development of the primed and antiviral states in IF-treated cells. It has been well known that certain metabolic activities such as RNA and protein syntheses are necessary for developing the antiviral state in cells [5 , 7, 12]. It is, therefore, of interest to determine whether these metabolic processes are also required in the priming process.We report here the effects of temperature, duration of IF treatment and metabolic inhibitors on the priming process in IF-treatied cells and discuss a possible mechanism of the priming action. MATERIALS AND METHODSCells and virus. Mouse L cells were grown in Eagle's minimal essential medium (MEM) supplemented with 5% calf serum (CS) and maintained in MEM supplemented with 2% CS.

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