Enhancing Effect of Daidzein on the Differentiation and Mineralization in Mouse Osteoblast-like MC3T3-E1 Cells

Abstract

The eŠect of daidzein, an important iso‰avone, on the diŠerentiation and mineralization in MC3T3 E1 cells, a mouse calvaria osteoblast-like cell line, was investigated. The MTT assay, the alizarin red S and von Kossa staining, the measurement of calcium (Ca) and phosphorus (P) concentrations by inductively coupled plasma-atomic emission spectrometry and the nitrophenol liberation method were used to determine the cell proliferation, mineralization and intracellular alkaline phosphatase (ALP) activity, respectively. Daidzein enhanced the cell proliferation after the culture for 2 days and the eŠect reached maximum on day 6. ALP activity and cellular Ca and P contents were increased timeand dose-dependently when the cells were treated with daidzein in the presence of disodium b-glycerophosphate and Lascorbic acid. DiŠerentiation of the cells to the mature osteoblasts was prompted under incubation in the presence of daidzein for 21 days, by the time the mineralized nodules formed. The calcium depositions of the cells by alizarin red S staining were increased signiˆcantly after the culture with daidzein as long as 28 days. It has been demonstrated that daidzein may be able to enhance the bone diŠerentiation and mineralization and prompt the bone formation in the early growing stage and the late growing stage of osteoblasts.

Daidzein promotes osteoblast proliferation and differentiation in OCT1 cells through stimulating the activation of BMP-2/Smads pathway

“…Strong et al (2014) reported that daidzein enhanced the expression of some ostegenic genes in human bone marrow-derived mesenchymal stem cells (HBMSCs), such as Osx, ALP, and OPN. The stimulatory role of daidzein on the osteoblast differentiation and mineralization in MC3T3-E1 cells has also been reported (Ge et al 2006). In this study, we show that daidzein stimulated osteoblast proliferation and differentiation in OCT1 cells.…”

“…Daidzein-enhanced osteoblast growth might be mediated through the upregulation of BMP expression in primary osteoblast cells (Jia et al, 2003). Daidzein stimulated cell differentiation and mineralization in mouse osteoblastlike MC3T3-E1 cells (Ge et al, 2006). The effects of daidzein on osteoblasts were mediated through the ERbeta pathway (De Wilde et al, 2004;de Wilde et al, 2006).…”

Phenolic phytochemicals and bone

“…Glycitein, at 10 -7 M, was also able to promote cell differentiation in MC3T3-E1 cells (Yoshida et al 2001). In addition, soy isoflavones (effective concentrations: 10 -7 to 10 -5 M) have been shown to improve not only differentiation but also mineralization in MC3T3-E1 cells (Kanno et al 2004;Ge et al 2006). Similarly, it was found that 1 lM of both genistein and daidzein could stimulate bonenodule formation in primary osteoblasts obtained from newborn Sprague-dawley rat calvarias (Chang et al 2003).…”

Soybean Phytoestrogens – Friends or Foes?

“…In addition, phytoestrogens such as coumestrol, genistein and daidzein increase alkaline phosphatase activity in osteoblast-like cells (Kanno et al, 2004). Daidzein stimulates osteoblast differentiation, induces changes in the action of the cytoskeleton responsible for cell adhesion and motility and activates transcription factors associated with cell proliferation and differentiation (de Wilde et al, 2004, Ge et al, 2006Jia et al, 2003). Also, isoflavones promote insulin-like growth factor-I (IGF-I) production which enhances osteoblastic activity (Ajrmandi et al, 2000) Isoflavones inhibit bone resorption, via direct targeting of osteoclasts.…”