Enhancing effect of calaguala on the prevention of rejection on skin transplants in mice

Tuominen, M.; Bohlin, Lars; Lindbom, Lars; Rolfsen, W

doi:10.1002/ptr.2650050510

Cited by 21 publications

(8 citation statements)

References 5 publications

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“…leucotornos extract is a hydroalcoholic extract containing a saponin formed by a ketosteroid and deoxyhexose with little published information on its composition and structure of active ingredient(s) (19). The extract has been shown to exhibit immunomodulating activities in vitro and in vivo, exemplified by an increase in number of lymphocytes CD8+ (20), a prolongation of the survival of skin allografts in mice (21), suppression of effector and memory T-cells and increase of NK activity, modulation of brain levels of some cytokines in rats (22), suppression of proliferative response to exogenous r-IL-2 and antiCD3 mononuclear antibodies (23), and inhibition of both the release of elastase in human neutrophils induced by platelet activating factor (PAF), and the biosynthesis of PAF (24). In recent years, Z?…”

Section: Polypodium {Eucotornosmentioning

confidence: 99%

Inhibition of ultraviolet‐induced formation of reactive oxygen species, lipid peroxidation, erythema and skin photosensitization by Polypodium leucotomos

González

Pathak

1996

Photoderm Photoimm Photomed

119

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The acute reactions of human skin to solar ultraviolet radiation (290-400 nm) are recognized as a form of inflammation reactions that are mediated by several possible mechanisms including (a) direct action of photons on DNA, (b) generation of reactive free radicals and reactive oxygen species involving the formation of O2.-, 1O2, H2O2, OH, etc., (c) generation of prostaglandins (PGD2, PGE2, etc.), histamine, leucotrienes, and other inflammatory mediators. It is conceivable that UV-induced reactions represent oxidative stress mediated by the formation of free radicals, reactive oxygen, lipid peroxidation, liberation of membrane phospholipids, and subsequent formation of prostaglandins by cyclo-oxygenase pathway. In this study, we examined the role of reactive oxygen species and lipid peroxidation in in vitro reactions as well as in vivo skin inflammation reactions induced by (a) UVB radiation (290-320 nm), and (b) skin photosensitization reaction by PUVA treatment involving 8-methoxypsoralen and UVA (320-400 nm) radiation and presented data for the generation of superoxide anion O2.-) and lipid peroxides. We have also evaluated, both in vitro as well as in vivo systems, the quenching or the inhibition of O2.- by a plant extract known as Polypodium leucotomos. The P. leucotomos extract was found to exhibit interesting antioxidant and anti-inflammatory as well as photoprotective properties against photo-oxidative stress involving the generation of reactive oxygen, lipid peroxidation under in vitro reactions as well as in vivo experimental conditions. Significant inhibition of UVB-induced erythemal response, and 8-methoxypsoralen plus UVA-induced phototoxic reaction after topical application or oral administration of the photosensitizer could be demonstrated in guinea pig skin and human skin following the topical application of P. leucotomos extract. The photoprotective mechanism of P.leucotomos involving interaction with reactive oxygen species or free radicals appears to have potential clinical usefulness in preventing sunburn and inhibiting phototoxic reaction.

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Section: Polypodium {Eucotornosmentioning

confidence: 99%

Inhibition of ultraviolet‐induced formation of reactive oxygen species, lipid peroxidation, erythema and skin photosensitization by Polypodium leucotomos

González

Pathak

1996

Photoderm Photoimm Photomed

119

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“…The first natural product evaluated in this study was an extract from Polypodium leucotomos (PL) that has been used in Central America for the treatment of several phlogistic disorders, including skin-associated inflammatory problems (psoriasis, atopic dermatitis) (22,23). PL has shown an antioxidant action in several studies (24).…”

Section: Introductionmentioning

confidence: 99%

The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species

Gomes

Lunardi

González

et al. 2001

Braz J Med Biol Res

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Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 10 9 M -1 s -1 for KA and 1.0 x 10 9 M -1 s -1 for PL, similar to that of ethanol (1.4 x 10 9 M -1 s -1 ). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited · O 2 -dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of 1 O 2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an antioxidant effect, scavenging three of four reactive oxygen species tested here. Unlike KA, PL did not significantly scavenge hydrogen peroxide. Correspondence

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“…In order to study whether an immunosuppressive activity could explain the observed effect of Calaguala in the treatment of psoriasis, a skin model of allograft rejection in mice was used. A methanolic extract of the leaves of the fern significantly prolonged the allograft survival compared with untreated transplanted mice and in the same order as the immunosuppressant drug cyclosporin A did (Tuominen et al ., ).…”

Section: Bioassay‐guided Isolationmentioning

confidence: 97%

Bioassays in Natural Product Research – Strategies and Methods in the Search for Anti‐inflammatory and Antimicrobial Activity

Strömstedt

Felth

Bohlin

2013

Phytochemical Analysis

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Enhancing effect of calaguala on the prevention of rejection on skin transplants in mice

Cited by 21 publications

References 5 publications

Inhibition of ultraviolet‐induced formation of reactive oxygen species, lipid peroxidation, erythema and skin photosensitization by Polypodium leucotomos

Inhibition of ultraviolet‐induced formation of reactive oxygen species, lipid peroxidation, erythema and skin photosensitization by Polypodium leucotomos

The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species

Bioassays in Natural Product Research – Strategies and Methods in the Search for Anti‐inflammatory and Antimicrobial Activity

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