Enhancing Drought Resistance and Yield of Wheat through Inoculation with Streptomyces pactum Act12 in Drought Field Environments

Abstract

Drought stress is the primary abiotic factor affecting wheat growth, development, and yield formation. The application of plant growth-promoting rhizobacteria (PGPR) represents an environmentally sustainable approach to mitigate the impacts of drought stress on wheat. This study conducted field experiments using two winter wheat varieties, the drought-sensitive variety Jimai 22 and the drought-resistant variety Chang 6878, aiming to investigate the effects of Streptomyces pactum Act12 inoculation on photosynthetic characteristics, physiological parameters, and yield traits during the jointing, heading, and middle-filling stages under drought stress. The results revealed that drought stresses significantly reduced chlorophyll content, leaf area, biomass, and yield in wheat, while Act12 inoculation significantly increased chlorophyll content, photosynthetic efficiency, antioxidant enzyme activity such as superoxide dismutase (SOD) and peroxidase (POD), osmolyte content (proline and soluble proteins), and decreased malondialdehyde (MDA) content. These combined effects alleviated drought stress, resulting in increased biomass and yield in wheat. Under drought stress, an increase in leaf proline content of 13.53% to 53.23% (Jimai 22) and 17.17% to 43.08% (Chang 6878) was observed upon Act12 inoculation. Moreover, a decrease in MDA content was recorded of 15.86% to 53.61% (Jimai 22) and 13.47% to 26.21% (Chang 6878). Notably, there was a corresponding increase in yield of 11.78% (Jimai 22) and 13.55% (Chang 6878). In addition, grain quality analysis revealed a significant improvement in grain hardness with Act12 inoculation. Therefore, Act12 demonstrates the potential for enhancing the sustainable development of wheat production in arid and semi-arid regions.