Enhancing Detection of Gonococcus in Ejaculates of Adult Males Using Sperm Dilution

Vicari, Enzo; Mongioí, Alessandro; Speciale, A; Caccamo, F; Calogero, Aldo E.; Gulizia, S.; Pellegrino, M. B.; Macchi, María Verónica Russo; D’Agata, Rosario

doi:10.3109/01485018608986919

Cited by 12 publications

(10 citation statements)

References 8 publications

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“…Treatment failures are common in prostatitis. It was been hypothesized that altered pharmacokinetics in the inflamed prostate gland might account for the treatment failure of clinically diagnosed chronic bacterial prostatitis [24]. In a chronically inflamed prostate gland, another difficulty in eradicating bacteria relates to the fact that micro-organisms are protected within infection-induced microcolonies and biofilms and, therefore, grow firmly attached to the inflamed ductal and acinar walls [25].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, an aliquot from all samples was cultured aerobically and anaerobically following a 1:2 dilution in saline solution, according to previously published standard bacteriological methods [3,24]. To minimize methodological errors, the same investigators, in a blinded fashion, performed all seminal analyses (E.V.…”

Section: Http://wwwasiaandrocom; Aja@sibsaccnmentioning

confidence: 99%

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Transrectal ultrasonography in infertile patients with persistently elevated bacteriospermia

Vignera¹,

Calogero²,

Arancio³

et al. 2008

Asian Journal of Andrology

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Section: Discussionmentioning

confidence: 99%

Section: Http://wwwasiaandrocom; Aja@sibsaccnmentioning

confidence: 99%

Transrectal ultrasonography in infertile patients with persistently elevated bacteriospermia

Vignera¹,

Calogero²,

Arancio³

et al. 2008

Asian Journal of Andrology

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“…One aliquot from each sample was cultured aerobically and anaerobically after diluting the samples (1:2) in saline solution. Standard bacteriological methods were used to quantify and identify all organisms according to previously published methods (Vicari et al ., ). All study subjects were also tested for infection with Chlamydia and Mycoplasma by analysing cultures of urethral fluids that were obtained by prostate massage and collected using swabs.…”

Section: Methodsmentioning

confidence: 97%

Relationship of semen hyperviscosity with IL-6, TNF-α, IL-10 and ROS production in seminal plasma of infertile patients with prostatitis and prostato-vesiculitis

et al. 2013

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Changes in levels of oxidative damage products in semen and their relationship to seminal fluid viscosity (SFV) have recently received increasing research interest. We analysed whether SFV was associated with ROS generation, levels of cytokines TNF-alpha (TNF-α), IL-6 and IL-10 and seminal leucocyte concentration, and whether ROS production was related to the extent of infections/inflammations at one (prostatitis) or two (prostato-vesiculitis) male accessory glands. We studied 169 infertile patients, with chronic bacterial prostatitis (PR, n = 74) and/or bilateral prostato-vesiculitis (PV, n = 95), as diagnosed by the ultrasound (US) criteria. Healthy fertile men (n = 42) served as controls. In the PV patient group, SFV, semen characteristics and ROS production had median values that were significantly higher than those found in PR patients and controls, although other sperm variables had values significantly lower than those found in PR patients or controls. In PV infertile patients, ROS generation and pro-inflammatory cytokines levels were higher than those found in PR infertile patients and controls, although seminal IL-10 levels in PV and PR patients were lower than those found in the controls. In PR patients, the levels of SFV were positively related to TNF-α (r = 0.67; P < 0.01), fMLP-stimulated ROS production in the 45% Percoll fraction (r = 0.687, P < 0.01) and the 90% Percoll fraction in basal condition (r = 0.695, P < 0.01), and after fMLP-stimulation (r = 0.688, P < 0.01). Thus, our data indicated that seminal hyperviscosity is associated with increased oxidative stress in infertile men and increased pro-inflammatory interleukins in patients with male accessory gland infection, more when the infection was extended to the seminal vesicles.

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“…Therefore, this condition was considered clinically as prostatitis stable compared with pretreatment phase with antibiotics. Standard bacteriological methods were used to quantify and identify all organisms, in one aliquot from each sample (derived from EPS or semen) cultured aerobically and anaerobically after diluting the samples (1:2) in saline solution, according to previously published methods 1920

Instead, progression into PV or PVE was located and verified when they showed clinical signs (palpation of tender prostate at the physical examination) and ultrasound findings considered indicative of PV or PVE, as previously described 21

…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chronic bacterial prostatitis and irritable bowel syndrome: effectiveness of treatment with rifaximin followed by the probiotic VSL#3

et al. 2014

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This study was undertaken to evaluate the influence of treatment with rifaximin followed by the probiotic VSL#3 versus no treatment on the progression of chronic prostatitis toward chronic microbial prostate-vesiculitis (PV) or prostate-vesiculo-epididymitis (PVE). A total of 106 selected infertile male patients with bacteriologically cured chronic bacterial prostatitis (CBP) and irritable bowel syndrome (IBS) were randomly prescribed rifaximin (200 mg, 2 tablets bid, for 7 days monthly for 12 months) and probiotic containing multiple strains VSL#3 (450 × 109 CFU per day) or no treatment. Ninety-five of them (89.6%) complied with the therapeutic plan and were included in this study. Group A = “6Tx/6-”: treatment for the initial 6 and no treatment for the following 6 months (n = 26); Group B = “12Tx”: 12 months of treatment (n = 22); Group C = “6-/6Tx”: no treatment for the initial 6 months and treatment in the last 6 months (n = 23); Group D = “12-”: no treatment (n = 24). The patients of Groups A = “6Tx/6-” and B = “12Tx” had the highest frequency of chronic prostatitis (88.5% and 86.4%, respectively). In contrast, group “12-”: patients had the lowest frequency of prostatitis (33.4%). The progression of prostatitis into PV in groups “6Tx/6-” (15.5%) and “6-/6Tx” (13.6%) was lower than that found in the patients of group “12-” (45.8%). Finally, no patient of groups “6Tx/6-” and “6-/6Tx” had PVE, whereas it was diagnosed in 20.8% of group “12-” patients. Long-term treatment with rifaximin and the probiotic VSL#3 is effective in lowering the progression of prostatitis into more complicated forms of male accessory gland infections in infertile patients with bacteriologically cured CBP plus IBS.

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Enhancing Detection of Gonococcus in Ejaculates of Adult Males Using Sperm Dilution

Cited by 12 publications

References 8 publications

Transrectal ultrasonography in infertile patients with persistently elevated bacteriospermia

Transrectal ultrasonography in infertile patients with persistently elevated bacteriospermia

Relationship of semen hyperviscosity with IL-6, TNF-α, IL-10 and ROS production in seminal plasma of infertile patients with prostatitis and prostato-vesiculitis

Chronic bacterial prostatitis and irritable bowel syndrome: effectiveness of treatment with rifaximin followed by the probiotic VSL#3

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