Enhancing chemosensitivity of wild-type and drug-resistant MDA-MB-231 triple-negative breast cancer cell line to doxorubicin by silencing of STAT 3, Notch-1, and β-catenin genes

Alkaraki, Arwa; Alshaer, Walhan; Wehaibi, Suha; Gharaibeh, Lobna; Abuarqoub, Duaa; Alqudah, Dana A.; Al-Azzawi, Hafsa; Zureigat, Hadil; Souleiman, Mamoun; Awidi, Abdalla

doi:10.1007/s12282-020-01098-9

Cited by 24 publications

(21 citation statements)

References 34 publications

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“…Cells were kept in a humidified 5% CO 2 at 37 °C. Doxorubicin-resistant MCF-7 (MCF-7/DR) and MDA-MB-231 (MDA-MB-231/DR) cell lines used in this work were previously developed in our lab and maintained using similar conditions of wild-type cell lines [ 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

“…Both RPMI-1640 and EMEM medium were supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) 200 mM L-glutamine, and antibiotics; Penicillin-Streptomycin (100 IU/mL-100 µg/mL). Cells were kept in a humidified 5% CO 2 at 37 • C. Doxorubicin-resistant MCF-7 (MCF-7/DR) and MDA-MB-231 (MDA-MB-231/DR) cell lines used in this work were previously developed in our lab and maintained using similar conditions of wild-type cell lines [46,47].…”

Section: Nanoparticle Tracking Analysis (Nta)mentioning

confidence: 99%

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Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer

et al. 2022

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Advanced innovations for combating variants of aggressive breast cancer and overcoming drug resistance are desired. In cancer treatment, ZnO nanoparticles (NPs) have the capacity to specifically and compellingly activate apoptosis of cancer cells. There is also a pressing need to develop innovative anti-cancer therapeutics, and recent research suggests that ZnO nanoparticles hold great potential. Here, the in vitro chemical effectiveness of ZnO NPs has been tested. Zinc oxide (ZnO) nanoparticles were synthesized using Citrullus colocynthis (L.) Schrad by green methods approach. The generated ZnO was observed to have a hexagonal wurtzite crystal arrangement. The generated nanomaterials were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), UV-visible spectroscopy. The crystallinity of ZnO was reported to be in the range 50–60 nm. The NPs morphology showed a strong absorbance at 374 nm with an estimated gap band of 3.20 eV to 3.32 eV. Microscopy analysis proved the morphology and distribution of the generated nanoparticles to be around 50 nm, with the elemental studies showing the elemental composition of ZnO and further confirming the purity of ZnO NPs. The cytotoxic effect of ZnO NPs was evaluated against wild-type and doxorubicin-resistant MCF-7 and MDA-MB-231 breast cancer cell lines. The results showed the ability of ZnO NPs to inhibit the prefoliation of MCF-7 and MDA-MB-231 prefoliation through the induction of apoptosis without significant differences in both wild-type and resistance to doxorubicin.

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Section: Methodsmentioning

confidence: 99%

Section: Nanoparticle Tracking Analysis (Nta)mentioning

confidence: 99%

Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer

et al. 2022

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“…On the basis of results of the optimized and characterized PLL loading amount in the particles depending on pH and incubation time, cell attachment tests were conducted to validate the enhanced cell adhesion capacity of the PEG‐based hydrogel microparticles by functionalization with PLL. As a cell model for cell adhesion experiments, we selected MDA‐MB‐231 breast cancer cells, which have been broadly researched for cell adhesion, 2 organoid culture, 39 and drug resistance tests 40 . In addition, a homemade PDMS elastomer reservoir was fabricated to attach cells with microparticles under static conditions by covering partially cured PDMS‐coated glass slides with perforated PDMS blocks, as with the SFL device fabrication process.…”

Section: Resultsmentioning

confidence: 99%

Direct functionalization of cell‐adhesion promoters to hydrogel microparticles synthesized by stop‐flow lithography

Jang

Kim

Mun

et al. 2022

Journal of Polymer Science

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Polyethylene glycol (PEG) hydrogel microparticles generated via stop‐flow lithography can be utilized for efficient microparticle‐based cell culture processes because of their high biocompatibility, the molecular diffusion capability in the gel structure, and the tunability of their shape and size. However, the typical functionalization process of PEG microparticles with cell‐adhesion promoters has inevitable limitations, requiring additional linker molecules and the preconjugation of linkers to cell‐adhesion promoters and microparticles. In this study, a simple and direct cell‐adhesion promoter functionalization process of the PEG microparticles is presented by use of aza‐Michael reaction between remnant unreacted acrylate groups in particles and amine groups in cell‐adhesion promoters. On the basis of proposed process, particles are directly conjugated with poly‐l‐lysine (PLL), a typical cell‐adhesion promoter that can electrostatically interact with cellular membranes, in a controllable manner. We demonstrate enhanced cell‐adhesion capabilities of the particles along with the increased amount of conjugated PLL in the particles. Furthermore, to validate extended applicability, the particles are directly conjugated with Gly‐Arg‐Gly‐Asp‐Ser (GRGDS) peptides, in which RGD sequence is involved in the cell‐adhesion behavior of extracellular matrix proteins, including fibronectin. The introduced GRGDS peptides increase the cell‐adhesion capacity of the microparticles binding to integrin proteins in cellular membranes.

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“…Indeed, the CD44 + CD24 -/low phenotype has been positively associated with tumor growth and migration in TNBC cells ( 51 ). Following doxorubicin treatment, doxorubicin-resistant MDA-MB-231 cells have substantially upregulated the CD44 + CD24 -/low phenotype compared to wild-type cells ( 52 ). Besides the TNBC cells, growing evidence indicates that the CD44 + CD24 -/low phenotype can promote EMT and chemoresistance in other cancers.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Review to Clarify the Prognostic Values of CD44 and CD44+CD24- Phenotype in Triple-Negative Breast Cancer Patients: Lessons Learned and The Road Ahead

et al. 2021

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As a unique population of tumor bulk, cancer stem cells have been implicated in tumor relapse and chemoresistance in triple-negative breast cancer (TNBC). Therefore, understanding the phenotype of cancer stem cells can pave the way for introducing novel molecular targeted therapies for treating TNBC patients. Preclinical studies have identified CD44+CD24-/low as a cancer stem cell phenotype; however, clinical studies have reported seemingly controversial results regarding the prognostic values of CD44 and CD44+CD24-/low phenotype in TNBC patients. To critically review the clinicopathological significance and prognostic values of CD44 and CD44+CD24-/low phenotype in TNBC patients, the Scopus, Embase, PubMed, and Web of Science databases were systematically searched to obtain the relevant records published before 20 October 2020. Based on nine included studies, CD44 and CD44+CD24-/low phenotype are associated with inferior prognosis in TNBC patients. Moreover, these cancer stem cell markers have been associated with advanced tumor stage, tumor size, higher tumor grade, tumor metastasis, and lymphatic involvement in TNBC patients. Our evidence has also indicated that, unlike the treatment-naïve TNBC patients, the tumoral cells of chemoradiotherapy-treated TNBC patients can upregulate the CD44+CD24-/low phenotype and establish an inverse association with androgen receptor (AR), leading to the inferior prognosis of affected patients. In summary, CD44 and CD44+CD24-/low phenotype can be utilized to determine TNBC patients’ prognosis in the pathology department as a routine practice, and targeting these phenotypes can substantially improve the prognosis of TNBC patients.

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Enhancing chemosensitivity of wild-type and drug-resistant MDA-MB-231 triple-negative breast cancer cell line to doxorubicin by silencing of STAT 3, Notch-1, and β-catenin genes

Cited by 24 publications

References 34 publications

Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer

Synthesis, Characterization, and Assessment of Anti-Cancer Potential of ZnO Nanoparticles in an In Vitro Model of Breast Cancer

Direct functionalization of cell‐adhesion promoters to hydrogel microparticles synthesized by stop‐flow lithography

A Systematic Review to Clarify the Prognostic Values of CD44 and CD44+CD24- Phenotype in Triple-Negative Breast Cancer Patients: Lessons Learned and The Road Ahead

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